Mechanism of Sphingosine 1-Phosphate- and Lysophosphatidic Acid-Induced Up-Regulation of Adhesion Molecules and Eosinophil Chemoattractant in Nerve Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. IMR32 Cells Express S1P1, S1P3 and LPA1–3; S1P and LPA Induce Up-Regulation of ICAM-1 Transcription
2.2. S1P and LPA Induce ERK Activation in IMR32 Cells with Different Time Courses with Consequenct Gi-Protein Coupled and ERK-Dependent Up-Regulation of ICAM-1 and/or CCL-26
2.3. The Eosinophil Granule Protein EPO Induces Up-Regulation of S1P1, LPA1, LPA2 and LPA3 Receptors Expression
3. Experimental Section
4. Conclusion
References
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Gene | Primer Sequence | Amplicon Length (Base Pairs) |
---|---|---|
S1P1 | 5′-ATCGTCCTGAGCGTCTTCAT-3′ (forward) 5′-CCAGGAAGTACTCCGCTCTG-3′ (reverse) | 95 |
S1P2 | 5′-CCAAGCATTATGTGCTGTGC-3′ (forward) 5′-CAGAAGGAGGATGCTGAAGG-3′ (reverse) | 186 |
S1P3 | 5′-ACCAGTACGTGGGGAAGTTG-3′ (forward) 5′-GGCAATCAAAACCATCAGGT-3′ (reverse) | 105 |
S1P4 | 5′-CCAAGCGCTACATCCTCTTC-3 (forward) 5′-CAGAGGTTGGAGCCAAAGAC-3′ (reverse) | 221 |
S1P5 | 5′-ACAACTACACCGGCAAGCTC-3′ (forward) 5′-GCCCCGACAGTAGGATGTT-3′ (reverse) | 218 |
LPA1 | 5′-ATTTCACAGCCCCAGTTCAC-3′ (forward) 5′-CACCAGCTTGCTGACTGTGT-3′ (reverse) | 106 |
LPA2 | 5′-CTGCTCCTGGATGGTTTAGG-3′ (forward) 5′-CTCGGCAAGAGTACACAGCA-3′ (reverse) | 95 |
LPA3 | 5′-TTGCCTCTGCAACATCTCTG-3′ (forward) 5′-ATGATGAGGAAGGCCATGAG-3 (reverse) | 82 |
ICAM-1 | 5′-CAAGGCCTCAGTCAGTGTGA-3′ (forward) 5′-CCTCTGGCTTCGTCAGAATC-3′ (reverse) | 129 |
CCL26 | 5′-CCTCCTGAGTCTCCACCTTG-3′ (forward) 5′-TGGGAGCAGCTGTTACTGGT-3′ (reverse) | 115 |
-actin | 5′-GGACTTCGAGCAAGAGATGG-3′ (forward) 5′-AGGAAGGAAGGCTGGAAGAG-3′ (reverse) | 118 |
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Costello, R.W.; Maloney, M.; Atiyeh, M.; Gleich, G.; Walsh, M.-T. Mechanism of Sphingosine 1-Phosphate- and Lysophosphatidic Acid-Induced Up-Regulation of Adhesion Molecules and Eosinophil Chemoattractant in Nerve Cells. Int. J. Mol. Sci. 2011, 12, 3237-3249. https://doi.org/10.3390/ijms12053237
Costello RW, Maloney M, Atiyeh M, Gleich G, Walsh M-T. Mechanism of Sphingosine 1-Phosphate- and Lysophosphatidic Acid-Induced Up-Regulation of Adhesion Molecules and Eosinophil Chemoattractant in Nerve Cells. International Journal of Molecular Sciences. 2011; 12(5):3237-3249. https://doi.org/10.3390/ijms12053237
Chicago/Turabian StyleCostello, Richard W., Michael Maloney, Mazin Atiyeh, Gerald Gleich, and Marie-Therese Walsh. 2011. "Mechanism of Sphingosine 1-Phosphate- and Lysophosphatidic Acid-Induced Up-Regulation of Adhesion Molecules and Eosinophil Chemoattractant in Nerve Cells" International Journal of Molecular Sciences 12, no. 5: 3237-3249. https://doi.org/10.3390/ijms12053237