A Critical Role for Cysteine 57 in the Biological Functions of Selenium Binding Protein-1
Abstract
:1. Introduction
2. Results
2.1. SBP1GLY Was Impaired in Its Ability to Protect HCT116 Cells from Selenite-Induced Toxicity
2.2. The Cytosolic Half-Life of SBP1 Is Affected by the Amino Acid at Position 57
2.3. Differential Affects of Wild Type SBP1 and SBP1GLY on Signaling Pathways Relevant to Carcinogenesis
2.4. Expression of SBP1GLY Induced Mitochondrial Damage in HCT116 Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. Plasmid Construction
4.3. Immunoblot Analysis
4.4. Measurement of Selenite-Induced Cytotoxicity
4.5. Determination of the SBP1 Half-Life
4.6. Mitochondrial Damage Assay
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Ying, Q.; Ansong, E.; Diamond, A.M.; Yang, W. A Critical Role for Cysteine 57 in the Biological Functions of Selenium Binding Protein-1. Int. J. Mol. Sci. 2015, 16, 27599-27608. https://doi.org/10.3390/ijms161126043
Ying Q, Ansong E, Diamond AM, Yang W. A Critical Role for Cysteine 57 in the Biological Functions of Selenium Binding Protein-1. International Journal of Molecular Sciences. 2015; 16(11):27599-27608. https://doi.org/10.3390/ijms161126043
Chicago/Turabian StyleYing, Qi, Emmanuel Ansong, Alan M. Diamond, and Wancai Yang. 2015. "A Critical Role for Cysteine 57 in the Biological Functions of Selenium Binding Protein-1" International Journal of Molecular Sciences 16, no. 11: 27599-27608. https://doi.org/10.3390/ijms161126043