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Article

Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia

by
Colby A. Souders
1,*,
Sharon E. Maynard
2,
Jing Yan
3,
Yang Wang
1,
Naomi K. Boatright
1,
Jessica Sedan
1,
David Balyozian
1,
Peter S. Cheslock
1,
Deborah C. Molrine
1 and
Tiffany A. Moore Simas
4,5
1
MassBiologics of the University of Massachusetts Medical School, Boston, MA 02126, USA
2
Department of Medicine, Division of Nephrology, Lehigh Valley Health Network, University of South Florida Morsani College of Medicine, Allentown, PA 18105, USA
3
Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01655, USA
4
Department of Obstetrics and Gynecology, University of Massachusetts Medical School/ UMass Memorial Health Care, Worcester, MA 01605, USA
5
Department of Pediatrics, University of Massachusetts Medical School, Worcester, MA 01655, USA
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2015, 16(6), 12436-12453; https://doi.org/10.3390/ijms160612436
Submission received: 1 April 2015 / Revised: 6 May 2015 / Accepted: 25 May 2015 / Published: 2 June 2015
(This article belongs to the Special Issue Prediction, Diagnostics and Prevention of Pregnancy Complications)
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Abstract

Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.
Keywords: preeclampsia; soluble fms-like tyrosine kinase 1 (sFlt1); splice variants; isoforms; monoclonal antibody (mAb); diagnostic preeclampsia; soluble fms-like tyrosine kinase 1 (sFlt1); splice variants; isoforms; monoclonal antibody (mAb); diagnostic

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MDPI and ACS Style

Souders, C.A.; Maynard, S.E.; Yan, J.; Wang, Y.; Boatright, N.K.; Sedan, J.; Balyozian, D.; Cheslock, P.S.; Molrine, D.C.; Simas, T.A.M. Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia. Int. J. Mol. Sci. 2015, 16, 12436-12453. https://doi.org/10.3390/ijms160612436

AMA Style

Souders CA, Maynard SE, Yan J, Wang Y, Boatright NK, Sedan J, Balyozian D, Cheslock PS, Molrine DC, Simas TAM. Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia. International Journal of Molecular Sciences. 2015; 16(6):12436-12453. https://doi.org/10.3390/ijms160612436

Chicago/Turabian Style

Souders, Colby A., Sharon E. Maynard, Jing Yan, Yang Wang, Naomi K. Boatright, Jessica Sedan, David Balyozian, Peter S. Cheslock, Deborah C. Molrine, and Tiffany A. Moore Simas. 2015. "Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia" International Journal of Molecular Sciences 16, no. 6: 12436-12453. https://doi.org/10.3390/ijms160612436

APA Style

Souders, C. A., Maynard, S. E., Yan, J., Wang, Y., Boatright, N. K., Sedan, J., Balyozian, D., Cheslock, P. S., Molrine, D. C., & Simas, T. A. M. (2015). Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia. International Journal of Molecular Sciences, 16(6), 12436-12453. https://doi.org/10.3390/ijms160612436

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