Differential miR-346 and miR-582-3p Expression in Association with Selected Maternal and Fetal Complications
Abstract
:1 Introduction
2. Results
2.1. Clinical Characteristics of Included Groups and Sample Collection
2.2. The Expression of miR-346 in Maternal Peripheral Blood, Fetal Cord Blood and Placenta
2.3. The Expression of miR-582-3p in Maternal Peripheral Blood, Fetal Cord Blood and Placenta
3. Discussion
4. Materials and Methods
4.1. Study Population and Sample Collection
4.2. miRNA Isolation and Quantitative Real-Time PCR (qRT-PCR)
4.3. Statistical Analyses
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Clinical Information | Normal Control (n = 60) | Preeclampsia (n = 31) | Preterm Delivery (n = 29) | Small for Gestational Age (n = 19) |
---|---|---|---|---|
Maternal Age (y) | 31.33 ± 4.31 | 33.83 ± 5.77 | 32.04 ± 5.14 | 31.68 ± 7.21 |
Nulliparity (%) | 56.7% | 48.4% | 34.5% * | 73.7% |
Gestational Age at Delivery (week) | 38.87 ± 0.99 | 36.84 ± 2.30 * | 34.73 ± 2.00 * | 36.87 ± 3.01 * |
Ethnicity (Chinese Hans %) | 100% | 100% | 96.3% | 89.5% |
Cesarean Section | 35.0% | 80.7% * | 51.9% | 47.4% |
BMI at Delivery (kg/m2) | 26.47 ± 3.55 | 29.10 ± 5.60 | 26.04 ± 4.46 | 24.67 ± 3.16 |
Neonatal Outcome | ||||
Birth Weight (g) | 3192 ± 299.59 | 2622 ± 676.74 * | 2214 ± 502.45 * | 2173 ± 598.61 * |
Sex (female %) | 50% | 50% | 51.7% | 57.9% |
1 min Apgar Score | 8.635 ± 0.71 | 8.063 ± 1.16 * | 7.36 ± 1.04 * | 7.938 ± 1.18 |
5 min Apgar Score | 9.827 ± 0.43 | 9.469 ± 0.88 | 9.160 ± 0.75 * | 9.438 ± 0.89 |
Placenta Weight (g) | 631.4 ± 113.95 | 560 ± 158.32 * | 562.1 ± 182.86 * | 437.4 ± 113.32 * |
Specimen Origin | Normal Control (n = 60) | Preeclampsia (n = 31) | Preterm Deliver (n = 29) | Small for Gestational Age (n = 19) |
---|---|---|---|---|
miR-346 | ||||
Maternal Plasma | 0.12 (0.02, 0.36) | 0.51 * (0.07, 1.00) | 2.11 * (0.63, 6.88) | 0.62 * (0.13, 1.56) |
Fetal Cord Plasma | 23.98 (4.37, 144.31) | 9.74 * (1.91, 21.89) | 7.21 (1.23, 44.09) | 5.96 (1.00, 25.00) |
Placenta | 1.00 (0.61, 2.06) | 0.54 * (0.27, 0.81) | 0.18 * (0.11, 1.00) | 0.56 * (0.26, 1.02) |
miR-582-3p | ||||
Maternal Plasma | 5.64 × 10−5 (0.07 × 10−5, 24.76 × 10−5) | 4.17 × 10−3 * (0.20 × 10−3, 16.31 × 10−3) | 6.09 × 10−2 * (0.08 × 10−2, 29.50 × 10−2) | 5.48 × 10−3 (0.02 × 10−1, 37.01 × 10−1) |
Fetal Cord Plasma | 1.67 × 10−3 (0.05 × 10−3, 56.60 × 10−3) | 1.14 × 10−2 (0.01 × 10−2, 6.72 × 10−2) | 9.28 × 10−3 (0.24 × 10−3, 469.52 × 10−3) | 1.02 × 10−3 (0.02 × 10−3, 17.39 × 10−3) |
Placenta | 1.56 (0.83, 2.45) | 0.75 (0.39, 11.37) | 0.23 * (0.14, 0.80) | 0.70 * (0.28, 0.95) |
Specimen Origin | Normal Control (n = 60) | Preeclampsia (n = 31) | Preterm Delivery (n = 29) | Small for Gestational Age (n = 19) | |||
---|---|---|---|---|---|---|---|
slope | p value | slope | p value | slope | p value | ||
miR-346 | |||||||
Log Maternal plasma | 1 | 1.13 * | 0.049 | 2.50 * | <0.001 | 1.16 | 0.068 |
Log Fetal cord plasma | 1 | −1.45 * | 0.008 | −1.81 * | 0.006 | −1.50 * | 0.015 |
Log Placenta | 1 | −0.61 | 0.059 | −1.53 * | <0.001 | −0.99 * | 0.007 |
miR-582-3p | |||||||
Log Maternal plasma | 1 | 3.14 * | 0.020 | 4.20 * | 0.010 | 2.44 | 0.099 |
Log Fetal cord plasma | 1 | 1.37 | 0.455 | 2.18 | 0.185 | −0.36 | 0.812 |
Log Placenta | 1 | 0.21 | 0.603 | −1.67* | <0.001 | −1.14 * | 0.012 |
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Tsai, P.-Y.; Li, S.-H.; Chen, W.-N.; Tsai, H.-L.; Su, M.-T. Differential miR-346 and miR-582-3p Expression in Association with Selected Maternal and Fetal Complications. Int. J. Mol. Sci. 2017, 18, 1570. https://doi.org/10.3390/ijms18071570
Tsai P-Y, Li S-H, Chen W-N, Tsai H-L, Su M-T. Differential miR-346 and miR-582-3p Expression in Association with Selected Maternal and Fetal Complications. International Journal of Molecular Sciences. 2017; 18(7):1570. https://doi.org/10.3390/ijms18071570
Chicago/Turabian StyleTsai, Pei-Yin, Sheng-Hsiang Li, Wan-Ni Chen, Hui-Ling Tsai, and Mei-Tsz Su. 2017. "Differential miR-346 and miR-582-3p Expression in Association with Selected Maternal and Fetal Complications" International Journal of Molecular Sciences 18, no. 7: 1570. https://doi.org/10.3390/ijms18071570