Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells
Abstract
:1. Introduction
2. Results
2.1. TPS Inhibited the Progression of Colitis Associated Colorectal (CAC) Cancer
2.2. TPS-Induced Apoptosis and Inhibited Cell Cycle Progression in CAC Mice
2.3. TPS Inhibited the Expression of Cyclin D1, MMP-2, and MMP-9 in CAC Mice
2.4. Effect of TPS on Cell Proliferation and Invasion of CT26 Cells In Vitro
2.5. TPS-Induced Apoptosis and Inhibited Cell Cycle Progression of CT26 Cells In Vitro
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Line and Conditioned Culture
4.3. Cell Viability and Proliferation Assays
4.4. Assessment of Cell Apoptosis by Annexin V-FITC/PI Double-Staining Assay
4.5. Analysis of Cell Cycle
4.6. Cell Matrigel Invasion Assay
4.7. AOM/DSS-Induced Colitis-Associated Colorectal Carcinogenesis and TPS Treatment
4.8. Immunohistochemistry
4.9. Terminal Deoxynucleotide Transferase (TdT) dUTP Nick End Labeling (TUNEL) Assay
4.10. Western Blot Analysis
4.11. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Group | Dose (mg/kg) | Colon Length (cm) | Tumor Number |
---|---|---|---|
AOM/DSS | 0 | 6.582 ± 0.250 | 16.667 ± 2.582 |
50 | 7.117 ± 0.380 | 11.667 ± 2.160 Δ | |
AOM/DSS + TPS | 100 | 7.747 ± 0.318 ΔΔ | 8.833 ± 1.472 ΔΔ |
200 | 8.272 ± 0.450 ΔΔ | 4.833 ± 1.722 ΔΔ |
Group | Dose (mg/kg) | Spleen Index (mg/g) | Thymus Index (mg/g) |
---|---|---|---|
Normal | 200 | 5.732 ± 0.359 ΔΔ | 1.773 ± 0.051 ΔΔ |
Normal + TPS | 0 | 6.455 ± 0.341 **Δ | 2.232 ± 0.090 **ΔΔ |
AOM/DSS | 0 | 7.035 ± 0.206 ** | 1.957 ± 0.181 |
50 | 6.843 ± 0.262 ** | 2.402 ± 0.250 **ΔΔ | |
AOM/DSS + TPS | 100 | 6.672 ± 0.229 ** | 2.408 ± 0.116 **ΔΔ |
200 | 6.510 ± 0.256 *Δ | 2.672 ± 0.093 **ΔΔ |
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Liu, L.-Q.; Li, H.-S.; Nie, S.-P.; Shen, M.-Y.; Hu, J.-L.; Xie, M.-Y. Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells. Int. J. Mol. Sci. 2018, 19, 506. https://doi.org/10.3390/ijms19020506
Liu L-Q, Li H-S, Nie S-P, Shen M-Y, Hu J-L, Xie M-Y. Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells. International Journal of Molecular Sciences. 2018; 19(2):506. https://doi.org/10.3390/ijms19020506
Chicago/Turabian StyleLiu, Li-Qiao, Hai-Shan Li, Shao-Ping Nie, Ming-Yue Shen, Jie-Lun Hu, and Ming-Yong Xie. 2018. "Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells" International Journal of Molecular Sciences 19, no. 2: 506. https://doi.org/10.3390/ijms19020506