Next Article in Journal
Ubidecarenone-Loaded Nanostructured Lipid Carrier (UB-NLC): Percutaneous Penetration and Protective Effects Against Hydrogen Peroxide-Induced Oxidative Stress on HaCaT Cells
Next Article in Special Issue
The Molecular Mechanisms of Mutations in Actin and Myosin that Cause Inherited Myopathy
Previous Article in Journal
Boldine Improves Kidney Damage in the Goldblatt 2K1C Model Avoiding the Increase in TGF-β
Previous Article in Special Issue
Synchrotron Radiation X-ray Diffraction Techniques Applied to Insect Flight Muscle
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle?

1
Department Chemistry Biomedical Sciences, Linnaeus University, SE-39182 Kalmar, Sweden
2
Department Kinesiology and Physical Education, McGill University, Montreal, QC H3A 2T5, Canada
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(7), 1863; https://doi.org/10.3390/ijms19071863
Submission received: 23 May 2018 / Revised: 19 June 2018 / Accepted: 20 June 2018 / Published: 25 June 2018
(This article belongs to the Special Issue The Actin-Myosin Interaction in Muscle)

Abstract

In muscle, but not in single-molecule mechanics studies, actin, myosin and accessory proteins are incorporated into a highly ordered myofilament lattice. In view of this difference we compare results from single-molecule studies and muscle mechanics and analyze to what degree data from the two types of studies agree with each other. There is reasonable correspondence in estimates of the cross-bridge power-stroke distance (7–13 nm), cross-bridge stiffness (~2 pN/nm) and average isometric force per cross-bridge (6–9 pN). Furthermore, models defined on the basis of single-molecule mechanics and solution biochemistry give good fits to experimental data from muscle. This suggests that the ordered myofilament lattice, accessory proteins and emergent effects of the sarcomere organization have only minor modulatory roles. However, such factors may be of greater importance under e.g., disease conditions. We also identify areas where single-molecule and muscle data are conflicting: (1) whether force generation is an Eyring or Kramers process with just one major power-stroke or several sub-strokes; (2) whether the myofilaments and the cross-bridges have Hookean or non-linear elasticity; (3) if individual myosin heads slip between actin sites under certain conditions, e.g., in lengthening; or (4) if the two heads of myosin cooperate.
Keywords: optical tweezers; optical traps; muscle fiber; myofibril; myosin; actin; cross-bridge; mechanochemical model optical tweezers; optical traps; muscle fiber; myofibril; myosin; actin; cross-bridge; mechanochemical model
Graphical Abstract

Share and Cite

MDPI and ACS Style

Månsson, A.; Ušaj, M.; Moretto, L.; Rassier, D.E. Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle? Int. J. Mol. Sci. 2018, 19, 1863. https://doi.org/10.3390/ijms19071863

AMA Style

Månsson A, Ušaj M, Moretto L, Rassier DE. Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle? International Journal of Molecular Sciences. 2018; 19(7):1863. https://doi.org/10.3390/ijms19071863

Chicago/Turabian Style

Månsson, Alf, Marko Ušaj, Luisa Moretto, and Dilson E. Rassier. 2018. "Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle?" International Journal of Molecular Sciences 19, no. 7: 1863. https://doi.org/10.3390/ijms19071863

APA Style

Månsson, A., Ušaj, M., Moretto, L., & Rassier, D. E. (2018). Do Actomyosin Single-Molecule Mechanics Data Predict Mechanics of Contracting Muscle? International Journal of Molecular Sciences, 19(7), 1863. https://doi.org/10.3390/ijms19071863

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop