Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2 Arabidopsis thaliana Katanin Mutant
Abstract
:1. Introduction
2. Results
2.1. Cell Wall Hemicellulose and Pectin Epitope Analysis of Uninfected Roots
2.2. Hemicellulose and Pectin Distribution Patterns of Giant Cell Walls of Col-0 and fra2 Roots
2.3. The Impact of Katanin Mutation on the Infection of A.thaliana by M. incognita
3. Discussion
4. Materials and Methods
4.1. Plant Growth Conditions, Nematode Rearing and Collection
4.2. Light and Fluorescence Microscopy
4.3. Cell Wall Epitope Immunolabeling
4.4. Arabidopsis Wild-Type and fra2 Mutant Nematode Susceptibility
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
RKN | Root-Knot Nematodes |
GCs | Giant Cells |
MT | Microtubules |
DeSPHG | De-esterified Pectic HomoGalacturonan |
MPHG | Methylesterified Pectic HomoGalacturonan |
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Cell Wall Components | A. thaliana | ||
---|---|---|---|
Col-0 GCs | fra2 GCs | ||
Hemicelluloses | Xyloglucans | +/+ | +/+ |
Callose | +/+ | − | |
Pectins | MPHG | +/− | +/+ |
DeSPHG | +/+ | +/+ | |
Arabinan | +/− | +/+ |
Antibody | Epitope | References |
---|---|---|
Hemicelluloses | ||
LM25 | Recognizes XLLG, XXLG and XXXG motifs of xyloglucans | [61] |
Anti-β-1,3-glucan | Recognizes 1,3 β-glucan | [57] |
Pectins | ||
LM20 | HG domain in pectic polysaccharides, requires methyl esters for recognition of HG and does not bind to unesterified HG | [59] |
JIM5 | HG domain of pectic polysaccharides, recognizes partially methyl-esterified epitopes of HG, can also bind to unesterified HG | [60] |
LM6 | Recognizes a linear pentasaccharide in (1-5)-α-L-arabinans | [59] |
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Meidani, C.; Ntalli, N.G.; Giannoutsou, E.; Adamakis, I.-D.S. Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2 Arabidopsis thaliana Katanin Mutant. Int. J. Mol. Sci. 2019, 20, 5465. https://doi.org/10.3390/ijms20215465
Meidani C, Ntalli NG, Giannoutsou E, Adamakis I-DS. Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2 Arabidopsis thaliana Katanin Mutant. International Journal of Molecular Sciences. 2019; 20(21):5465. https://doi.org/10.3390/ijms20215465
Chicago/Turabian StyleMeidani, Christianna, Nikoletta G. Ntalli, Eleni Giannoutsou, and Ioannis-Dimosthenis S. Adamakis. 2019. "Cell Wall Modifications in Giant Cells Induced by the Plant Parasitic Nematode Meloidogyne incognita in Wild-Type (Col-0) and the fra2 Arabidopsis thaliana Katanin Mutant" International Journal of Molecular Sciences 20, no. 21: 5465. https://doi.org/10.3390/ijms20215465