Treatment with Peanut Sprout Root Extract Alleviates Inflammation in a Lipopolysaccharide-Stimulated Mouse Macrophage Cell Line by Inhibiting the MAPK Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Chemical Profile of PSRE
2.2. Effect of PSRE on Viability, NO Production and NF-κB Activation of RAW264.7 Macrophages
2.3. Effect of PSRE on the Expression of Inflammatory Cytokines in RAW264.7 Macrophages
2.4. Effect of PSRE on COX-2 and iNOS Protein Expression
2.5. Effect of PSRE on MAPK Phosphorylation
3. Discussion
4. Materials and Methods
4.1. PSRE Preparation
4.2. Chemical Profiling of PSRE
4.3. Cell Culture
4.4. Cell Viability
4.5. NO and Inflammatory Cytokine Assays
4.6. Western Blot Analysis
4.7. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PSRE | Peanut Sprouts Root Extract |
LPS | Lipopolysaccharide |
NO | Nitric oxide |
TNF-α | Tumor necrosis factor-α |
IL-1β | Interleukin-1β |
IL-6 | Interleukin-6 |
PGE2 | Prostaglandin E2 |
iNOS | Inducible nitric oxide synthase |
COX-2 | Cyclooxygenase 2 |
MAPK | Mitogen-activated protein kinase |
ERK | Extracellular signal-regulated kinase |
JNK | c-Jun n-terminal kinase |
References
- Ye, H.; Wang, Y.; Yan, J.; Jenson, A.B. Characterization of the anti-inflammation mechanism for the AO herbal extract. Exp. Mol. Pathol. 2016, 101, 341–345. [Google Scholar] [CrossRef] [PubMed]
- Kim, K.N.; Ko, Y.J.; Kang, M.C.; Yang, H.M.; Roh, S.W.; Oda, T.; Jeon, Y.J.; Jung, W.K.; Heo, S.J.; Yoon, W.J.; et al. Anti-inflammatory effects of trans-1,3-diphenyl-2,3-epoxypropane-1-one mediated by suppression of inflammatory mediators in LPS-stimulated RAW 264.7 macrophages. Food Chem. Toxicol.: Int. J. Publ. Br. Ind. Biol. Res. Assoc. 2013, 53, 371–375. [Google Scholar] [CrossRef] [PubMed]
- Koh, T.J.; DiPietro, L.A. Inflammation and wound healing: The role of the macrophage. Expert Rev. Mol. Med. 2011, 13, e23. [Google Scholar] [CrossRef] [PubMed]
- Jung, C.H.; Jung, H.; Shin, Y.C.; Park, J.H.; Jun, C.Y.; Kim, H.M.; Yim, H.S.; Shin, M.G.; Bae, H.S.; Kim, S.H.; et al. Eleutherococcus senticosus extract attenuates LPS-induced iNOS expression through the inhibition of Akt and JNK pathways in murine macrophage. J. Ethnopharmacol. 2007, 113, 183–187. [Google Scholar] [CrossRef] [PubMed]
- Cho, B.O.; Ryu, H.W.; So, Y.; Lee, C.W.; Jin, C.H.; Yook, H.S.; Jeong, Y.W.; Park, J.C.; Jeong, I.Y. Anti-Inflammatory Effect of Mangostenone F in Lipopolysaccharide-Stimulated RAW264.7 Macrophages by Suppressing NF-kappaB and MAPK Activation. Biomol. Ther. 2014, 22, 288–294. [Google Scholar] [CrossRef]
- Wang, K.H.; Lai, Y.H.; Chang, J.C.; Ko, T.F.; Shyu, S.L.; Chiou, R.Y. Germination of peanut kernels to enhance resveratrol biosynthesis and prepare sprouts as a functional vegetable. J. Agric. Food Chem. 2005, 53, 242–246. [Google Scholar] [CrossRef]
- Youn, C.K.; Jo, E.R.; Sim, J.H.; Cho, S.I. Peanut sprout extract attenuates cisplatin-induced ototoxicity by induction of the Akt/Nrf2-mediated redox pathway. Int. J. Pediatric Otorhinolaryngol. 2017, 92, 61–66. [Google Scholar] [CrossRef]
- Kang, N.E.; Ha, A.W.; Woo, H.W.; Kim, W.K. Peanut sprouts extract (Arachis hypogaea L.) has anti-obesity effects by controlling the protein expressions of PPARgamma and adiponectin of adipose tissue in rats fed high-fat diet. Nutr. Res. Pract. 2014, 8, 158–164. [Google Scholar] [CrossRef]
- Choi, D.I.; Choi, J.Y.; Kim, Y.J.; Lee, J.B.; Kim, S.O.; Shin, H.T.; Lee, S.C. Ethanol Extract of Peanut Sprout Exhibits a Potent Anti-Inflammatory Activity in Both an Oxazolone-Induced Contact Dermatitis Mouse Model and Compound 48/80-Treated HaCaT Cells. Ann. Dermatol. 2015, 27, 142–151. [Google Scholar] [CrossRef]
- Guha, M.; Mackman, N. LPS induction of gene expression in human monocytes. Cell. Signal. 2001, 13, 85–94. [Google Scholar] [CrossRef]
- Hiraiwa, K.; van Eeden, S.F. Contribution of lung macrophages to the inflammatory responses induced by exposure to air pollutants. Mediat. Inflamm. 2013, 2013, 619523. [Google Scholar] [CrossRef] [PubMed]
- Laskin, D.L.; Sunil, V.R.; Gardner, C.R.; Laskin, J.D. Macrophages and tissue injury: Agents of defense or destruction? Annu. Rev. Pharmacol. Toxicol. 2011, 51, 267–288. [Google Scholar] [CrossRef] [PubMed]
- McNelis, J.C.; Olefsky, J.M. Macrophages, immunity, and metabolic disease. Immunity 2014, 41, 36–48. [Google Scholar] [CrossRef] [PubMed]
- Raetz, C.R.; Whitfield, C. Lipopolysaccharide endotoxins. Annu. Rev. Biochem. 2002, 71, 635–700. [Google Scholar] [CrossRef] [PubMed]
- Cho, B.O.; So, Y.; Jin, C.H.; Nam, B.M.; Yee, S.T.; Jeong, I.Y. 3-deoxysilybin exerts anti-inflammatory effects by suppressing NF-kappaB activation in lipopolysaccharide-stimulated RAW264.7 macrophages. Biosci. Biotechnol. Biochem. 2014, 78, 2051–2058. [Google Scholar] [CrossRef] [PubMed]
- Lee, J.H.; Ko, J.Y.; Samarakoon, K.; Oh, J.Y.; Heo, S.J.; Kim, C.Y.; Nah, J.W.; Jang, M.K.; Lee, J.S.; Jeon, Y.J. Preparative isolation of sargachromanol E from Sargassum siliquastrum by centrifugal partition chromatography and its anti-inflammatory activity. Food Chem. Toxicol. Int. J. Publ. Br. Ind. Biol. Res. Assoc. 2013, 62, 54–60. [Google Scholar] [CrossRef] [PubMed]
- Chen, L.; Deng, H.; Cui, H.; Fang, J.; Zuo, Z.; Deng, J.; Li, Y.; Wang, X.; Zhao, L. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget 2018, 9, 7204–7218. [Google Scholar] [CrossRef]
- Zelova, H.; Hosek, J. TNF-alpha signalling and inflammation: Interactions between old acquaintances. Inflamm. Res. Off. J. Eur. Histamine Res. Soc. 2013, 62, 641–651. [Google Scholar] [CrossRef]
- Lima-Junior, D.S.; Costa, D.L.; Carregaro, V.; Cunha, L.D.; Silva, A.L.; Mineo, T.W.; Gutierrez, F.R.; Bellio, M.; Bortoluci, K.R.; Flavell, R.A.; et al. Inflammasome-derived IL-1beta production induces nitric oxide-mediated resistance to Leishmania. Nat. Med. 2013, 19, 909–915. [Google Scholar] [CrossRef]
- Barnes, T.C.; Anderson, M.E.; Moots, R.J. The many faces of interleukin-6: The role of IL-6 in inflammation, vasculopathy, and fibrosis in systemic sclerosis. Int. J. Rheumatol. 2011, 2011, 721608. [Google Scholar] [CrossRef]
- Chen, Y.C.; Shen, S.C.; Lee, W.R.; Hou, W.C.; Yang, L.L.; Lee, T.J. Inhibition of nitric oxide synthase inhibitors and lipopolysaccharide induced inducible NOS and cyclooxygenase-2 gene expressions by rutin, quercetin, and quercetin pentaacetate in RAW 264.7 macrophages. J. Cell. Biochem. 2001, 82, 537–548. [Google Scholar] [CrossRef] [PubMed]
- Buapool, D.; Mongkol, N.; Chantimal, J.; Roytrakul, S.; Srisook, E.; Srisook, K. Molecular mechanism of anti-inflammatory activity of Pluchea indica leaves in macrophages RAW 264.7 and its action in animal models ofinflammation. J. Ethnopharmacol. 2013, 146, 495–504. [Google Scholar] [CrossRef] [PubMed]
- Chun, J.; Choi, R.J.; Khan, S.; Lee, D.S.; Kim, Y.C.; Nam, Y.J.; Lee, D.U.; Kim, Y.S. Alantolactone suppresses inducible nitric oxide synthase and cyclooxygenase-2 expression by down-regulating NF-kappaB, MAPK and AP-1 via the MyD88 signaling pathway in LPS-activated RAW 264.7 cells. Int. Immunopharmacol. 2012, 14, 375–383. [Google Scholar] [CrossRef] [PubMed]
- Yang, Y.; Kim, S.C.; Yu, T.; Yi, Y.S.; Rhee, M.H.; Sung, G.H.; Yoo, B.C.; Cho, J.Y. Functional roles of p38 mitogen-activated protein kinase in macrophage-mediated inflammatory responses. Mediat. Inflamm. 2014, 2014, 352371. [Google Scholar] [CrossRef]
- Bonizzi, G.; Karin, M. The two NF-kappaB activation pathways and their role in innate and adaptive immunity. Trends Immunol. 2004, 25, 280–288. [Google Scholar] [CrossRef]
- Bose, S.; Kar, N.; Maitra, R.; DiDonato, J.A.; Banerjee, A.K. Temporal activation of NF-kappaB regulates an interferon-independent innate antiviral response against cytoplasmic RNA viruses. Proc. Natl. Acad. Sci. USA 2003, 100, 10890–10895. [Google Scholar] [CrossRef]
- Sohn, K.H.; Jo, M.J.; Cho, W.J.; Lee, J.R.; Cho, I.J.; Kim, S.C.; Kim, Y.W.; Jee, S.Y. Bojesodok-eum, a Herbal Prescription, Ameliorates Acute Inflammation in Association with the Inhibition of NF-kappaB-Mediated Nitric Oxide and ProInflammatory Cytokine Production. Evid. Based Complementary Altern. Med. Ecam 2012, 2012, 457370. [Google Scholar] [CrossRef]
- Kim, H.J.; Park, K.J.; Lim, J.H. Metabolomic analysis of phenolic compounds in buckwheat (Fagopyrum esculentum M.) sprouts treated with methyl jasmonate. J. Agric. Food Chem. 2011, 59, 5707–5713. [Google Scholar] [CrossRef]
- Limmongkon, A.; Nopprang, P.; Chaikeandee, P.; Somboon, T.; Wongshaya, P.; Pilaisangsuree, V. LC-MS/MS profiles and interrelationships between the anti-inflammatory activity, total phenolic content and antioxidant potential of Kalasin 2 cultivar peanut sprout crude extract. Food Chem. 2018, 239, 569–578. [Google Scholar] [CrossRef]
- Tsai, K.L.; Kao, C.L.; Hung, C.H.; Cheng, Y.H.; Lin, H.C.; Chu, P.M. Chicoric acid is a potent anti-atherosclerotic ingredient by anti-oxidant action and anti-inflammation capacity. Oncotarget 2017, 8, 29600–29612. [Google Scholar] [CrossRef]
- Ding, H.; Ci, X.; Cheng, H.; Yu, Q.; Li, D. Chicoric acid alleviates lipopolysaccharide-induced acute lung injury in mice through anti-inflammatory and anti-oxidant activities. Int. Immunopharmacol. 2019, 66, 169–176. [Google Scholar] [CrossRef] [PubMed]
- Lee, N.Y.; Chung, K.S.; Jin, J.S.; Bang, K.S.; Eom, Y.J.; Hong, C.H.; Nugroho, A.; Park, H.J.; An, H.J. Effect of Chicoric Acid on Mast Cell-Mediated Allergic Inflammation in Vitro and in Vivo. J. Nat. Prod. 2015, 78, 2956–2962. [Google Scholar] [CrossRef] [PubMed]
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Lee, Y.M.; Son, E.; Kim, D.-S. Treatment with Peanut Sprout Root Extract Alleviates Inflammation in a Lipopolysaccharide-Stimulated Mouse Macrophage Cell Line by Inhibiting the MAPK Signaling Pathway. Int. J. Mol. Sci. 2019, 20, 5907. https://doi.org/10.3390/ijms20235907
Lee YM, Son E, Kim D-S. Treatment with Peanut Sprout Root Extract Alleviates Inflammation in a Lipopolysaccharide-Stimulated Mouse Macrophage Cell Line by Inhibiting the MAPK Signaling Pathway. International Journal of Molecular Sciences. 2019; 20(23):5907. https://doi.org/10.3390/ijms20235907
Chicago/Turabian StyleLee, Yun Mi, Eunjung Son, and Dong-Seon Kim. 2019. "Treatment with Peanut Sprout Root Extract Alleviates Inflammation in a Lipopolysaccharide-Stimulated Mouse Macrophage Cell Line by Inhibiting the MAPK Signaling Pathway" International Journal of Molecular Sciences 20, no. 23: 5907. https://doi.org/10.3390/ijms20235907