Molecular Cytogenetic Identification of Wheat-Aegilops Biuncialis 5Mb Disomic Addition Line with Tenacious and Black Glumes
Abstract
:1. Introduction
2. Results
2.1. Genomic In Situ Hybridization (GISH) Analysis
2.2. Non-Denaturing Fluorescence In Situ Hybridization (ND-FISH) Analysis
2.3. Multicolor GISH (mcGISH) Analysis
2.4. Development of Ae. Biuncialis Specific Markers and Molecular Detection of Line WA317
2.5. The Agronomic Traits of Line WA317
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. GISH Analysis
4.3. Non-Denaturing FISH Analysis
4.4. Multicolor GISH (mcGISH) Analysis
4.5. Development of Ae. Biuncialis Specific Markers and Identification of The Addition Line WA317
4.6. Agronomic Trait Determination of WA317
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CS | Chinese Spring |
DAPI | 4,6-Diamidino-2-phenylindole |
GISH | Genomic in situ hybridization |
ISH | in situ hybridization |
ND-FISH | Non-denaturing fluorescence in situ hybridization |
SLAF | Specific-locus amplified fragment sequencing |
PCR | Polymerase chain reaction |
References
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Type | Ae. Biuncialis Y17 (UUMM) | Ae. Umbellulata Y139 (UU) | Ae. Comosa Y258 (MM) | No. of Specific Markers | No. of WA317 Specific Markers |
---|---|---|---|---|---|
Type 1: U and M shared | + | + | + | 172 | 37 |
Type 2: U-specific | + | + | - | 6 | 0 |
Type 3: M-specific | + | - | + | 102 | 6 |
Type 4: Y17-specific | + | - | - | 18 | 2 |
Trait | 2017–2018 Season | 2018–2019 Season | ||
---|---|---|---|---|
CS | WA317 | CS | WA317 | |
Plant height (cm) | 114.60 ± 2.30 | 81.00 ± 3.35 ** | 120.25 ± 4.02 | 82.90 ± 5.02 ** |
Valid spikes per plant | 11.83 ± 0.98 | 10.80 ± 0.84 | 12.16 ± 0.98 | 10.50 ± 1.22 |
Spike length | 7.80 ± 0.34 | 7.50 ± 0.72 | 8.23 ± 0.65 | 8.00 ± 0.19 |
Spikelet number per spike | 21.43 ± 1.51 | 19.20 ± 1.09 | 22.67 ± 1.37 | 20.80 ± 0.75 |
Grain number per spike | 63.00 ± 4.64 | 42.60 ± 5.94 ** | 63.33 ± 4.63 | 44.00 ± 3.69 ** |
Thousand grain weight (g) | 36.81 ± 1.56 | 30.77 ± 2.81 ** | 34.56 ± 0.42 | 26.05 ± 1.01 ** |
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Song, L.; Zhao, H.; Zhang, Z.; Zhang, S.; Liu, J.; Zhang, W.; Zhang, N.; Ji, J.; Li, L.; Li, J. Molecular Cytogenetic Identification of Wheat-Aegilops Biuncialis 5Mb Disomic Addition Line with Tenacious and Black Glumes. Int. J. Mol. Sci. 2020, 21, 4053. https://doi.org/10.3390/ijms21114053
Song L, Zhao H, Zhang Z, Zhang S, Liu J, Zhang W, Zhang N, Ji J, Li L, Li J. Molecular Cytogenetic Identification of Wheat-Aegilops Biuncialis 5Mb Disomic Addition Line with Tenacious and Black Glumes. International Journal of Molecular Sciences. 2020; 21(11):4053. https://doi.org/10.3390/ijms21114053
Chicago/Turabian StyleSong, Liqiang, Hui Zhao, Zhi Zhang, Shuai Zhang, Jiajia Liu, Wei Zhang, Na Zhang, Jun Ji, Lihui Li, and Junming Li. 2020. "Molecular Cytogenetic Identification of Wheat-Aegilops Biuncialis 5Mb Disomic Addition Line with Tenacious and Black Glumes" International Journal of Molecular Sciences 21, no. 11: 4053. https://doi.org/10.3390/ijms21114053