Porphyromonas gingivalis HmuY and Streptococcus gordonii GAPDH—Novel Heme Acquisition Strategy in the Oral Microbiome
Abstract
:1. Introduction
2. Results and Discussion
2.1. S. gordonii SgGAPDH Binds Heme
2.2. S. gordonii SgGAPDH Could be a Player in Heme Acquisition Strategy Used by Periodontopathogens
2.3. P. gingivalis HmuY May Participate in Co-Aggregation and Biofilm Formation with S. gordonii
3. Materials and Methods
3.1. Bacterial Strains and Growth Conditions
3.2. Co-Aggregation Assay
3.3. Overexpression, Purification and Site-Directed Mutagenesis
3.4. Susceptibility of SgGAPDH to Proteolytic Digestion
3.5. Analysis of Heme Binding
3.6. Detection of SgGAPDH Protein
3.7. Heme Sequestration Experiments
3.8. Reverse Transcriptase-Quantitative Polymerase Chain Reaction (RT-qPCR)
3.9. Circular Dichroism (CD) Analysis
3.10. Bioinformatics and Statistical Analyses
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
SgGAPDH | Streptococcus gordonii glyceraldehyde-3-phosphate dehydrogenase |
HmuY | Porphyromonas gingivalis hemophore-like heme-binding protein |
Tfo | HmuY homolog from Tannerella forsythia |
PinO | HmuY homolog from Prevotella intermedia |
PinA | HmuY homolog from Prevotella intermedia |
Kgp | Porphyromonas gingivalis lysine-specific gingipain |
RT-qPCR | reverse transcriptase-quantitative polymerase chain reaction |
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Ślęzak, P.; Śmiga, M.; Smalley, J.W.; Siemińska, K.; Olczak, T. Porphyromonas gingivalis HmuY and Streptococcus gordonii GAPDH—Novel Heme Acquisition Strategy in the Oral Microbiome. Int. J. Mol. Sci. 2020, 21, 4150. https://doi.org/10.3390/ijms21114150
Ślęzak P, Śmiga M, Smalley JW, Siemińska K, Olczak T. Porphyromonas gingivalis HmuY and Streptococcus gordonii GAPDH—Novel Heme Acquisition Strategy in the Oral Microbiome. International Journal of Molecular Sciences. 2020; 21(11):4150. https://doi.org/10.3390/ijms21114150
Chicago/Turabian StyleŚlęzak, Paulina, Michał Śmiga, John W. Smalley, Klaudia Siemińska, and Teresa Olczak. 2020. "Porphyromonas gingivalis HmuY and Streptococcus gordonii GAPDH—Novel Heme Acquisition Strategy in the Oral Microbiome" International Journal of Molecular Sciences 21, no. 11: 4150. https://doi.org/10.3390/ijms21114150