Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry
Abstract
:1. Introduction
2. Results
2.1. Arachidonic Acid (AA) Is Unable to Induce Ca2+ Mobilization in MCF10A and MDA-MB-231 Cells
2.2. MDA-MB-231 Cells Lack Functional Native Arachidonate-Regulated Ca2+-Selective (ARC) Channels
2.3. Effect of AA in MDA-MB-231 Cell Proliferation
2.4. AA Inhibits MDA-MB-231 Cell Migration
2.5. AA Attenuates MDA-MB-231 Cell Viability
2.6. AA Induces Mitochondrial Membrane Depolarization and Caspase Activation
3. Discussion
4. Materials and Methods
4.1. Material and Cell Lines
4.2. Determination of the Changes in The Cytosolic Free-Ca2+ Concentration ([Ca2+]c)
4.3. Western Blotting (WB)
4.4. Cell Proliferation Assay
4.5. Migration Assay
4.6. Cell Death and Mitochondrial Potential Depolarization Analysis
4.7. Analysis of Caspase Activity
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Cantonero, C.; Sánchez-Collado, J.; Lopez, J.J.; Salido, G.M.; Rosado, J.A.; Redondo, P.C. Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry. Int. J. Mol. Sci. 2020, 21, 3315. https://doi.org/10.3390/ijms21093315
Cantonero C, Sánchez-Collado J, Lopez JJ, Salido GM, Rosado JA, Redondo PC. Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry. International Journal of Molecular Sciences. 2020; 21(9):3315. https://doi.org/10.3390/ijms21093315
Chicago/Turabian StyleCantonero, Carlos, Jose Sánchez-Collado, Jose J. Lopez, Ginés M. Salido, Juan A. Rosado, and Pedro C. Redondo. 2020. "Arachidonic Acid Attenuates Cell Proliferation, Migration and Viability by a Mechanism Independent on Calcium Entry" International Journal of Molecular Sciences 21, no. 9: 3315. https://doi.org/10.3390/ijms21093315