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Volume 22
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10.3390/ijms22115959
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Article

Nuclear Magnetic Resonance Therapy Modulates the miRNA Profile in Human Primary OA Chondrocytes and Antagonizes Inflammation in Tc28/2a Cells

by
Bibiane Steinecker-Frohnwieser
1,
Birgit Lohberger
1,2,*,
Nicole Eck
1,2,
Anda Mann
3,
Cornelia Kratschmann
3,
Andreas Leithner
2,
Werner Kullich
1 and
Lukas Weigl
3
1
Ludwig Boltzmann Institute for Arthritis and Rehabilitation, 5760 Saalfelden, Austria
2
Department of Orthopedics and Trauma, Medical University Graz, 8036 Graz, Austria
3
Division of Special Anaesthesia and Pain Medicine, Medical University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(11), 5959; https://doi.org/10.3390/ijms22115959
Submission received: 15 April 2021 / Revised: 19 May 2021 / Accepted: 24 May 2021 / Published: 31 May 2021
(This article belongs to the Special Issue Molecular Connection between the Endocrine System and Body Regulation)
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Abstract

Nuclear magnetic resonance therapy (NMRT) is discussed as a participant in repair processes regarding cartilage and as an influence in pain signaling. To substantiate the application of NMRT, the underlying mechanisms at the cellular level were studied. In this study microRNA (miR) was extracted from human primary healthy and osteoarthritis (OA) chondrocytes after NMR treatment and was sequenced by the Ion PI Hi-Q™ Sequencing 200 system. In addition, T/C-28a2 chondrocytes grown under hypoxic conditions were studied for IL-1β induced changes in expression on RNA and protein level. HDAC activity an NAD(+)/NADH was measured by luminescence detection. In OA chondrocytes miR-106a, miR-27a, miR-34b, miR-365a and miR-424 were downregulated. This downregulation was reversed by NMRT. miR-365a-5p is known to directly target HDAC and NF-ĸB, and a decrease in HDAC activity by NMRT was detected. NAD+/NADH was reduced by NMR treatment in OA chondrocytes. Under hypoxic conditions NMRT changed the expression profile of HIF1, HIF2, IGF2, MMP3, MMP13, and RUNX1. We conclude that NMRT changes the miR profile and modulates the HDAC and the NAD(+)/NADH signaling in human chondrocytes. These findings underline once more that NMRT counteracts IL-1β induced changes by reducing catabolic effects, thereby decreasing inflammatory mechanisms under OA by changing NF-ĸB signaling.
Keywords: NMRT; osteoarthritis; miR; MMPs; inflammation NMRT; osteoarthritis; miR; MMPs; inflammation

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MDPI and ACS Style

Steinecker-Frohnwieser, B.; Lohberger, B.; Eck, N.; Mann, A.; Kratschmann, C.; Leithner, A.; Kullich, W.; Weigl, L. Nuclear Magnetic Resonance Therapy Modulates the miRNA Profile in Human Primary OA Chondrocytes and Antagonizes Inflammation in Tc28/2a Cells. Int. J. Mol. Sci. 2021, 22, 5959. https://doi.org/10.3390/ijms22115959

AMA Style

Steinecker-Frohnwieser B, Lohberger B, Eck N, Mann A, Kratschmann C, Leithner A, Kullich W, Weigl L. Nuclear Magnetic Resonance Therapy Modulates the miRNA Profile in Human Primary OA Chondrocytes and Antagonizes Inflammation in Tc28/2a Cells. International Journal of Molecular Sciences. 2021; 22(11):5959. https://doi.org/10.3390/ijms22115959

Chicago/Turabian Style

Steinecker-Frohnwieser, Bibiane, Birgit Lohberger, Nicole Eck, Anda Mann, Cornelia Kratschmann, Andreas Leithner, Werner Kullich, and Lukas Weigl. 2021. "Nuclear Magnetic Resonance Therapy Modulates the miRNA Profile in Human Primary OA Chondrocytes and Antagonizes Inflammation in Tc28/2a Cells" International Journal of Molecular Sciences 22, no. 11: 5959. https://doi.org/10.3390/ijms22115959

APA Style

Steinecker-Frohnwieser, B., Lohberger, B., Eck, N., Mann, A., Kratschmann, C., Leithner, A., Kullich, W., & Weigl, L. (2021). Nuclear Magnetic Resonance Therapy Modulates the miRNA Profile in Human Primary OA Chondrocytes and Antagonizes Inflammation in Tc28/2a Cells. International Journal of Molecular Sciences, 22(11), 5959. https://doi.org/10.3390/ijms22115959

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