Effect of Short-Term Cold Treatment on Carbohydrate Metabolism in Potato Leaves
Abstract
:1. Introduction
2. Results
2.1. The Relative Water Content under Cold Treatment
2.2. Expression of NADH Dehydrogenase in Cold-Stressed Plants
2.3. Malondialdehyde and Chlorophyll Content under Cold Treatment
2.4. Soluble Sugar Content under Cold Treatment
2.5. Expression of Both StGWD and StPWD under Cold Stress
2.6. Glucan Phosphorylases, Amylases, and the Disproportionating Enzyme 2 Activities under Cold Treatment
2.7. The Acid Invertase Activity under Cold Treatment
3. Discussion
4. Materials and Methods
4.1. Plant Material and Cold Treatment
4.2. Relative Water Content (RWC)
4.3. RNA Extraction
4.4. Two-Step Real-Time PCR
4.5. Carbohydrate Analysis
4.6. Chloroplast Isolation
4.7. Leaf Crude Extract Preparation
4.8. Determination of Chlorophyll, Malondialdehyde, and Protein Content
4.9. Amylase, Glucan Phosphorylase, and Disproportioning Enzyme 2 Zymography
4.10. Measurement of Acid Invertase Activity
4.11. Statistical Analyses
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
BAM | Beta-amylase |
DPE | Disproportionating enzyme |
GWD | Glucan, water dikinase |
PHO | Glucan phosphorylase |
NDA1 | Homolog of NADH dehydrogenase |
MDA | Malondialdehyde |
PWD | Phosphoglucan, water dikinase |
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Orzechowski, S.; Sitnicka, D.; Grabowska, A.; Compart, J.; Fettke, J.; Zdunek-Zastocka, E. Effect of Short-Term Cold Treatment on Carbohydrate Metabolism in Potato Leaves. Int. J. Mol. Sci. 2021, 22, 7203. https://doi.org/10.3390/ijms22137203
Orzechowski S, Sitnicka D, Grabowska A, Compart J, Fettke J, Zdunek-Zastocka E. Effect of Short-Term Cold Treatment on Carbohydrate Metabolism in Potato Leaves. International Journal of Molecular Sciences. 2021; 22(13):7203. https://doi.org/10.3390/ijms22137203
Chicago/Turabian StyleOrzechowski, Sławomir, Dorota Sitnicka, Agnieszka Grabowska, Julia Compart, Joerg Fettke, and Edyta Zdunek-Zastocka. 2021. "Effect of Short-Term Cold Treatment on Carbohydrate Metabolism in Potato Leaves" International Journal of Molecular Sciences 22, no. 13: 7203. https://doi.org/10.3390/ijms22137203