Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-Deoxy-7-amido-7-deazaguanosine-Modified DNA
Abstract
:1. Introduction
2. Results
2.1. Phage Morphology, Host Range and Physiological Characteristics
2.2. Overview of Genome
2.3. Structural Proteins
2.4. Packaging
2.5. DNA RRR
2.6. Transcription, Translation, Nucleotide Metabolism and DNA Modification
2.7. Lysis Cassette
2.8. Phylogenetic Analysis
3. Discussion
4. Materials and Methods
4.1. Phages and Bacterial Strains
4.2. Phage Isolation, Propagation and Purification Techniques
4.3. Transmission Electron Microscopy
4.4. DNA Isolation and Restriction Analysis
4.5. Genome Sequencing and Analysis
4.6. Analysis of Structural Proteins
4.7. LC–MS/MS Analysis of Phage DNA Modifications
4.8. Bioinformatic Analysis of DpdA-Like Proteins
4.9. Nucleotide Sequence Accession Numbers
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Šimoliūnienė, M.; Žukauskienė, E.; Truncaitė, L.; Cui, L.; Hutinet, G.; Kazlauskas, D.; Kaupinis, A.; Skapas, M.; Crécy-Lagard, V.d.; Dedon, P.C.; et al. Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-Deoxy-7-amido-7-deazaguanosine-Modified DNA. Int. J. Mol. Sci. 2021, 22, 7333. https://doi.org/10.3390/ijms22147333
Šimoliūnienė M, Žukauskienė E, Truncaitė L, Cui L, Hutinet G, Kazlauskas D, Kaupinis A, Skapas M, Crécy-Lagard Vd, Dedon PC, et al. Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-Deoxy-7-amido-7-deazaguanosine-Modified DNA. International Journal of Molecular Sciences. 2021; 22(14):7333. https://doi.org/10.3390/ijms22147333
Chicago/Turabian StyleŠimoliūnienė, Monika, Emilija Žukauskienė, Lidija Truncaitė, Liang Cui, Geoffrey Hutinet, Darius Kazlauskas, Algirdas Kaupinis, Martynas Skapas, Valérie de Crécy-Lagard, Peter C. Dedon, and et al. 2021. "Pantoea Bacteriophage vB_PagS_MED16—A Siphovirus Containing a 2′-Deoxy-7-amido-7-deazaguanosine-Modified DNA" International Journal of Molecular Sciences 22, no. 14: 7333. https://doi.org/10.3390/ijms22147333