Nuclear Transfer Arrest Embryos Show Massive Dysregulation of Genes Involved in Transcription Pathways
Abstract
:1. Introduction
2. Results
2.1. Incomplete Activation of Transcription Pathways in SCNT Embryos
2.2. Abnormal Transcription Pathway was Related to Massive Dysregulation of Genes in NTA Embryos
2.3. Defective Activation of Transcription Pathways Downstream GRNs in NTA Embryos
2.4. NTA Embryos Showed Weak Coordination of Key Predicted Target Genes
3. Discussion
4. Materials and Methods
4.1. Dataset Collection
4.2. RNA-seq Data Processing
4.3. Transcription-Related Pathways Selection
4.4. Differential Genes Expression Analysis
4.5. Definition of Transcription Related Gene Rescue in NTB Embryos
4.6. Single-Cell Gene Regulatory Network Inference
4.7. Functional Pathways Enrichment and Statistical Analysis
4.8. Data Visualization
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Long, C.; Li, H.; Li, X.; Yang, W.; Zuo, Y. Nuclear Transfer Arrest Embryos Show Massive Dysregulation of Genes Involved in Transcription Pathways. Int. J. Mol. Sci. 2021, 22, 8187. https://doi.org/10.3390/ijms22158187
Long C, Li H, Li X, Yang W, Zuo Y. Nuclear Transfer Arrest Embryos Show Massive Dysregulation of Genes Involved in Transcription Pathways. International Journal of Molecular Sciences. 2021; 22(15):8187. https://doi.org/10.3390/ijms22158187
Chicago/Turabian StyleLong, Chunshen, Hanshuang Li, Xinru Li, Wuritu Yang, and Yongchun Zuo. 2021. "Nuclear Transfer Arrest Embryos Show Massive Dysregulation of Genes Involved in Transcription Pathways" International Journal of Molecular Sciences 22, no. 15: 8187. https://doi.org/10.3390/ijms22158187