Mechanisms of Viscous Media Effects on Elementary Steps of Bacterial Bioluminescent Reaction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Experimental Procedures
2.3. Mathematical Modelling of the Reaction Kinetics
2.4. Data Analysis
2.5. Computational Analysis of Bacterial Luciferase—Cosolvents Interactions
3. Results
3.1. Kinetic Effects of Media with Glycerol and Sucrose on the Bioluminescent Reaction Catalyzed by Bacterial Luciferase
3.2. Mathematical Modeling of the Kinetics of the Bacterial Bioluminescent Reaction
3.3. Dependence of the Individual Rate Constants on Media Viscosity
3.4. Effects of Glycerol and Sucrose Molecules on Bacterial Luciferase Structure
3.5. The Dependence of Luciferase Kinetics on the Molecular Size of Viscogenic Cosolvent
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Rate Constant * | Buffer | Cosolvent | 10% | 20% | 30% | 40% | |
---|---|---|---|---|---|---|---|
, s | 7.85 | Glycerol | 7.50 | 5.05 | 3.66 | 2.60 | 0.81 ± 0.05 |
Sucrose | 5.69 | 4.25 | 3.29 | 2.64 | |||
, s | 0.15 | Glycerol | 0.15 | 0.15 | 0.12 | 0.10 | 0.84 ± 0.11 |
Sucrose | 0.15 | 0.12 | 0.09 | 0.05 | 0.82 ± 0.06 | ||
, Ms | 1.55 | Glycerol | 1.06 | 0.62 | 0.38 | 0.19 | 1.62 ± 0.04 |
Sucrose | 1.21 | 0.82 | 0.47 | 0.29 | 0.94 ± 0.03 | ||
, s | 400 | Glycerol | 398 | 403 | 403 | 395 | N/A |
Sucrose | 401 | 403 | 393 | 402 | N/A | ||
, Ms | 0.42 | Glycerol | 0.53 | 0.30 | 0.21 | 0.10 | 1.21 ± 0.35 |
Sucrose | 0.31 | 0.18 | 0.06 | 0.02 | 1.85 ± 0.15 | ||
, s | 10.90 | Glycerol | 8.95 | 7.50 | 5.42 | 5.30 | 0.59 ± 0.07 |
Sucrose | 7.02 | 9.07 | 9.40 | 4.67 | N/A | ||
, s | 0.46 | Glycerol | 0.45 | 0.47 | 0.43 | 0.42 | N/A |
Sucrose | 0.38 | 0.64 | 1.69 | 5.13 | N/A |
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Lisitsa, A.E.; Sukovatyi, L.A.; Bartsev, S.I.; Deeva, A.A.; Kratasyuk, V.A.; Nemtseva, E.V. Mechanisms of Viscous Media Effects on Elementary Steps of Bacterial Bioluminescent Reaction. Int. J. Mol. Sci. 2021, 22, 8827. https://doi.org/10.3390/ijms22168827
Lisitsa AE, Sukovatyi LA, Bartsev SI, Deeva AA, Kratasyuk VA, Nemtseva EV. Mechanisms of Viscous Media Effects on Elementary Steps of Bacterial Bioluminescent Reaction. International Journal of Molecular Sciences. 2021; 22(16):8827. https://doi.org/10.3390/ijms22168827
Chicago/Turabian StyleLisitsa, Albert E., Lev A. Sukovatyi, Sergey I. Bartsev, Anna A. Deeva, Valentina A. Kratasyuk, and Elena V. Nemtseva. 2021. "Mechanisms of Viscous Media Effects on Elementary Steps of Bacterial Bioluminescent Reaction" International Journal of Molecular Sciences 22, no. 16: 8827. https://doi.org/10.3390/ijms22168827