Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery
Abstract
:1. Introduction
2. Results
2.1. Drebrin Localizes to the AChR Compartment of Neuromuscular Junctions and to the Contractile Machinery of Muscle Sarcomeres
2.2. Drebrin Localizes to the Postsynaptic Machinery In Vitro
2.3. Drebrin Downregulation or Impairment of Drebrin Binding to F-Actin Compromises AChR Clustering In Vitro
2.4. Drebrin Regulates Microtubule Capture at AChR Clusters
2.5. Cell Surface Delivery of AChRs Is Not Affected by Drebrin Knockdown
2.6. Drebrin Cooperates with AChR Clustering Regulator Rapsyn
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cell and Tissue Labeling
4.3. Microscopy and Image Analysis
4.4. Transfection with siRNA and Plasmids
4.5. Generation of Stable C2C12 Line Overexpressing EB3-GFP
4.6. AChR Pull-Down and Co-Immunoprecipitation
4.7. Western Blot
4.8. Muscle Electroporation
4.9. RNA Isolation and Quantitative PCR
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
AChR | acetylcholine receptors |
BSA | bovine serum albumin |
BTP2 | 3,5-bis-trifluoromethyl pyrazole |
BTX | bungarotoxin |
CLASP2 | CLIP-associating protein 2 |
CNS | central nervous system |
DGC | dystrophin-glycoprotein complex |
DMEM | in Dulbecco’s modified Eagle’s medium |
DMSO | dimethyl sulfoxide |
EB1/EB3 | end-binding protein 1 or 3 |
ECM | extracellular matrix |
EGFP | Enhanced green fluorescent protein |
ELK | ETS-like protein 1, ETS domain transcription factor 1 |
FBS | foetal bovine serum |
GAPDH | glyceraldehyde-3-phosphate dehydrogenase |
GEF | guanine nucleotide-exchange factor |
HEK293 | human embryonic kidney cells 293 |
LL5β | pleckstrin homology-like domain family B member 2 |
MACF1 | microtubule-actin cross-linking factor 1 |
MT | microtubule |
NGS | normal goat serum |
NMJ | neuromuscular junctions |
PBS | phosphate buffered saline |
PFA | paraformaldehyde |
PIP3 | phosphatidylinositol-3,4,5-triphosphate |
TBST | Tris buffered saline with Tween |
TIP | plus-end-tracking proteins |
WAVE1 | WASP-family verprolin-homologous protein 1 |
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Alvarez-Suarez, P.; Nowak, N.; Protasiuk-Filipunas, A.; Yamazaki, H.; Prószyński, T.J.; Gawor, M. Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery. Int. J. Mol. Sci. 2021, 22, 9387. https://doi.org/10.3390/ijms22179387
Alvarez-Suarez P, Nowak N, Protasiuk-Filipunas A, Yamazaki H, Prószyński TJ, Gawor M. Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery. International Journal of Molecular Sciences. 2021; 22(17):9387. https://doi.org/10.3390/ijms22179387
Chicago/Turabian StyleAlvarez-Suarez, Paloma, Natalia Nowak, Anna Protasiuk-Filipunas, Hiroyuki Yamazaki, Tomasz J. Prószyński, and Marta Gawor. 2021. "Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery" International Journal of Molecular Sciences 22, no. 17: 9387. https://doi.org/10.3390/ijms22179387