Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells
Abstract
:1. Introduction
2. Results
2.1. High Prevalence of CeD in the Czech Republic with Study Participants Representing Autoimmune Disease Susceptibility across Familial Generations
2.2. Differential Expression of Transmembrane Markers Indicates Monocytes Extracted from CeD Patients Have the Ability to Transform into Dendritic Cells after Exposure to LPS/PTG
2.3. Identification of a Novel CD33:CD64 Bio-Profile Marker for CeD Patients
2.4. MoDCs from CeD Patients Exhibit Hypersensitivity to Gliadin Activation
2.5. Superior CD86 Presentation in MoDCs from CeD Patients Compared to CTL
2.6. Predominant Membrane-Associated HLA-DQ Expression in MoDCs Originating from CeD Patients Compared to CTL
3. Discussion
4. Materials and Methods
4.1. Clinical Diagnosis of CeD Patients and Pedigree Overview
4.2. Monocyte Isolation from Peripheral Blood Mononuclear Cells
4.3. Generation of Monocyte Derived Dendritic Cells (MoDCs)
4.4. Flow Cytometry and FACS Staining
4.5. Immunohistochemical and Confocal Microscopy Detection of MoDC Surface Antigens
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Hudec, M.; Riegerová, K.; Pala, J.; Kútna, V.; Černá, M.; O´Leary, V.B. Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells. Int. J. Mol. Sci. 2021, 22, 9982. https://doi.org/10.3390/ijms22189982
Hudec M, Riegerová K, Pala J, Kútna V, Černá M, O´Leary VB. Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells. International Journal of Molecular Sciences. 2021; 22(18):9982. https://doi.org/10.3390/ijms22189982
Chicago/Turabian StyleHudec, Michael, Kamila Riegerová, Jan Pala, Viera Kútna, Marie Černá, and Valerie Bríd O´Leary. 2021. "Celiac Disease Defined by Over-Sensitivity to Gliadin Activation and Superior Antigen Presentation of Dendritic Cells" International Journal of Molecular Sciences 22, no. 18: 9982. https://doi.org/10.3390/ijms22189982