Age-Related Changes in the Matrisome of the Mouse Skeletal Muscle
Abstract
:1. Introduction
2. Results
2.1. Identification of Proteins in the Gastrocnemius Muscle
2.2. Matrisome Quantification: Old SM vs. Adult SM
2.3. Ultrastructural Morphology and Morphometrical Evaluation
2.4. Evaluation of ECM Components by Immunofluorescence
3. Discussion
3.1. Alteration of Core Matrisome in Aged vs. Adult Mice
3.2. Alteration of Matrisome-Associated Proteins in Aged vs. Adult Mice
4. Materials and Methods
4.1. Reagents
4.2. Mice
4.3. Proteomic Analysis
4.3.1. Preparation of Protein Samples for Proteomic Analysis
4.3.2. Liquid Chromatography with Tandem Mass Spectrometry (LC-MS/MS)
4.3.3. Data Processing for Protein Identification and Quantification
4.4. Bioinformatic Analysis
4.5. ECM Component Evaluation by Immunofluorescence
4.6. Ultrastructural Morphological and Morphometrical Evaluation
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Protein Symbol | Protein Name | Protein Symbol | Protein Name |
---|---|---|---|
MATRISOME DIVISION: CORE MATRISOME | |||
Category: collagen | |||
CO1A1 | Collagen alpha-1(I) chain | CO6A1 | Collagen alpha-1(VI) chain |
CO1A2 | Collagen alpha-2(I) chain | CO6A2 | Collagen alpha-2(VI) chain |
CO3A1 | Collagen alpha-1(III) chain | CO6A6 | Collagen alpha-6(VI) chain |
CO4A1 | Collagen alpha-1(IV) chain | COBA1 | Collagen alpha-1(XI) chain |
CO4A2 | Collagen alpha-2(IV) chain | COEA1 | Collagen alpha-1(XIV) chain |
CO5A1 | Collagen alpha-1(V) chain | COFA1 | Collagen alpha-1(XV) chain |
CO5A2 | Collagen alpha-2(V) chain | COIA1 | Collagen alpha-1(XVIII) chain |
Category: ECM-glycoproteins | |||
ADIPO | Adiponectin | LAMB1 | Laminin subunit beta-1 |
AGRIN | Agrin | LAMB2 | Laminin subunit beta-2 |
BGH3 | Transforming growth factor-beta-induced protein ig-h3 | LAMC1 | Laminin subunit gamma-1 |
CILP1 | Cartilage intermediate layer protein 1 | MFAP2 | Microfibrillar-associated protein 2 |
CILP2 | Cartilage intermediate layer protein 2 | MFAP4 | Microfibril-associated glycoprotein 4 |
COMP | Cartilage oligomeric matrix protein | MFAP5 | Microfibrillar-associated protein 5 |
CREL1 | Cysteine-rich with EGF-like domain protein 1 | MFGM | Lactadherin |
DERM | Dermatopontin | NID1 | Nidogen-1 |
FBN1 | Fibrillin-1 | NID2 | Nidogen-2 |
FBN2 | Fibrillin-2 | POSTN | Periostin |
FIBA | Fibrinogen alpha chain | SLIT2 | Slit homolog 2 protein |
FIBB | Fibrinogen beta chain | SRPX | Sushi-repeat-containing protein SRPX |
FIBG | Fibrinogen gamma chain | SSPO | SCO-spondin |
FINC | Fibronectin | TENA | Tenascin |
IGS10 | Immunoglobulin superfamily member 10 | TINAL | Tubulointerstitial nephritis antigen-like |
LAMA2 | Laminin subunit alpha-2 | TSP1 | Thrombospondin-1 |
LAMA3 | Laminin subunit alpha-3 | TSP4 | Thrombospondin-4 |
LAMA4 | Laminin subunit alpha-4 | VMA5A | von Willebrand factor A domain-containing protein 5A |
LAMA5 | Laminin subunit alpha-5 | VWA1 | von Willebrand factor A domain-containing protein 1 |
Category: Proteoglycans | |||
ASPN | Asporin | PGBM | Basement membrane-specific heparan sulfate proteoglycan core protein |
CHADL | Chondroadherin-like protein | PGS1 | Biglycan |
FMOD | Fibromodulin | PGS2 | Decorin |
LUM | Lumican | PRELP | Prolargin |
MIME | Mimecan | PRG2 | Bone marrow proteoglycan |
MATRISOME DIVISION: MATRISOME-ASSOCIATED PROTEINS | |||
Category: ECM affiliated proteins | |||
ANXA1 | Annexin A1 | GPC1 | Glypican-1 |
ANXA2 | Annexin A2 | HEMO | Hemopexin |
ANXA3 | Annexin A3 | LEG1 | Galectin-1 |
ANXA4 | Annexin A4 | LEGL | Galectin-related protein |
ANXA5 | Annexin A5 | LMAN1 | Protein ERGIC-53 |
ANXA6 | Annexin A6 | PLXA4 | Plexin-A4 |
ANXA7 | Annexin A7 | PLXB3 | Plexin-B3 |
ANX11 | Annexin A11 | ||
Category: ECM regulators | |||
A1AT1 | Alpha-1-antitrypsin 1-1 | ITIH1 | Inter-alpha-trypsin inhibitor heavy chain H1 |
A1AT2 | Alpha-1-antitrypsin 1-2 | ITIH2 | Inter-alpha-trypsin inhibitor heavy chain H2 |
A1AT3 | Alpha-1-antitrypsin 1-3 | ITIH3 | Inter-alpha-trypsin inhibitor heavy chain H3 |
A1AT4 | Alpha-1-antitrypsin 1-4 | ITIH4 | Inter alpha-trypsin inhibitor, heavy chain 4 |
A2AP | Alpha-2-antiplasmin | ITIH5 | Inter-alpha-trypsin inhibitor heavy chain H5 |
AMBP | Protein AMBP | KNG1 | Kininogen-1 |
ANGT | Angiotensinogen | MMP17 | Matrix metalloproteinase-17 |
ANT3 | Antithrombin-III | PEDF | Pigment epithelium-derived factor |
CATB | Cathepsin B | PLMN | Plasminogen |
CATD | Cathepsin D | PZP | Pregnancy zone protein |
CBG | Corticosteroid-binding globulin | SERPH | Serpin H1 |
CPN2 | Carboxypeptidase N subunit 2 | SPA3K | Serine protease inhibitor A3K |
CYTB | Cystatin-B | SPA3M | Serine protease inhibitor A3M |
FA12 | Coagulation factor XII | SPA3N | Serine protease inhibitor A3N |
HEP2 | Heparin cofactor 2 | SPI2 | Serpin I2 |
HRG | Histidine-rich glycoprotein | SULF2 | Extracellular sulfatase Sulf-2 |
HYAL2 | Hyaluronidase-2 | TGM2 | Protein-glutamine gamma-glutamyltransferase 2 |
IC1 | Plasma protease C1 inhibitor | THRB | Prothrombin |
ILEUA | Leukocyte elastase inhibitor A | ||
Category: secreted factors | |||
ANGL7 | Angiopoietin-related protein 7 | S10A1 | Protein S100-A1 |
FILA2 | Filaggrin-2 | S10A4 | Protein S100-A4 |
HGFA | Hepatocyte growth factor activator | S10A6 | Protein S100-A6 |
INHBA | Inhibin beta A chain | S10AA | Protein S100-A10 |
MEG11 | Multiple epidermal growth factor-like domains protein 11 | WN10A | Protein Wnt-10a |
PBS Extract | ||||
---|---|---|---|---|
Category | Protein Symbol | Protein Name | Ratio (Old/Adult) Log2 Fold Change | p-Value |
ECM regulators | A1AT2 | Alpha-1-antitrypsin 1-2 | 0.69 | 0.006 |
A2AP | Alpha-2-antiplasmin | 1.04 | 0.038 | |
CATD | Cathepsin D | 0.58 | 0.001 | |
CBG | Corticosteroid-binding globulin | 1.82 | 0.049 | |
ILEUA | Leukocyte elastase inhibitor A | 1.11 | 0.008 | |
ITIH2 | Inter-alpha-trypsin inhibitor heavy chain H2 | 2.00 | 0.038 | |
ITIH4 | Inter alpha-trypsin inhibitor, heavy chain 4 | 1.42 | 0.018 | |
KNG1 | Kininogen-1 | 0.97 | 0.043 | |
PEDF | Pigment epithelium-derived factor | 1.11 | 0.040 | |
PZP | Pregnancy zone protein | 0.86 | 0.012 | |
SPA3N | Serine protease inhibitor A3N | 1.77 | 0.028 | |
ECM glycoproteins | FIBA | Fibrinogen alpha chain | 1.19 | 0.001 |
FIBB | Fibrinogen beta chain | 1.19 | 0.001 | |
FIBG | Fibrinogen gamma chain | 1.14 | 0.001 | |
TSP4 | Thrombospondin-4 | 1.44 | 0.000 | |
VMA5A | von Willebrand factor A domain-containing protein 5A | 0.81 | 0.000 | |
Proteoglycans | ASPN | Asporin | 0.53 | 0.004 |
LUM | Lumican | 0.48 | 0.030 | |
PRELP | Prolargin | 0.37 | 0.001 | |
ECM affiliated proteins | ANXA4 | Annexin A4 | 0.63 | 0.002 |
ANXA5 | Annexin A5 | 0.51 | 0.009 | |
ANXA6 | Annexin A6 | −0.39 | 0.008 | |
ANX11 | Annexin A11 | 0.28 | 0.021 | |
HEMO | Hemopexin | 0.74 | 0.018 | |
U/T Extract | ||||
Collagen | CO4A1 | Collagen alpha-1(IV) chain | 1.30 | 0.016 |
CO6A1 | Collagen alpha-1(VI) chain | 0.61 | 0.000 | |
CO6A2 | Collagen alpha-2(VI) chain | 0.59 | 0.001 | |
ECM regulators | A1AT2 | Alpha-1-antitrypsin 1-2 | 0.87 | 0.022 |
CATD | Cathepsin D | 1.04 | 0.000 | |
TGM2 | Protein-glutamine gamma-glutamyltransferase 2 | 0.80 | 0.013 | |
ECM glycoproteins | BGH3 | Transforming growth factor-beta-induced protein ig-h3 | 0.78 | 0.016 |
CILP1 | Cartilage intermediate layer protein 1 | 1.47 | 0.000 | |
DERM | Dermatopontin | 0.51 | 0.004 | |
FINC | Fibronectin | 1.35 | 0.036 | |
LAMA2 | Laminin subunit alpha-2 | 0.45 | 0.000 | |
LAMA5 | Laminin subunit alpha-5 | 0.79 | 0.002 | |
LAMB1 | Laminin subunit beta-1 | 0.47 | 0.010 | |
LAMB2 | Laminin subunit beta-2 | 0.53 | 0.000 | |
LAMC1 | Laminin subunit gamma-1 | 0.56 | 0.000 | |
NID1 | Nidogen-1 | 0.54 | 0.000 | |
NID2 | Nidogen-2 | 1.09 | 0.000 | |
POSTN | Periostin | 1.28 | 0.003 | |
TENA | Tenascin | 2.55 | 0.022 | |
TSP4 | Thrombospondin-4 | 1.34 | 0.048 | |
Proteoglycans | ASPN | Asporin | 0.79 | 0.008 |
FMOD | Fibromodulin | 0.73 | 0.005 | |
LUM | Lumican | 0.81 | 0.001 | |
MIME | Mimecan | 0.54 | 0.008 | |
PGS1 | Biglycan | 1.05 | 0.002 | |
PRELP | Prolargin | 0.87 | 0.007 | |
ECM affiliated proteins | ANXA2 | Annexin A2 | 0.42 | 0.005 |
ANXA5 | Annexin A5 | 2.28 | 0.002 | |
HEMO | Hemopexin | 1.59 | 0.007 | |
ANX11 | Annexin A11 | 0.90 | 0.000 | |
LEG1 | Galectin-1 | 1.19 | 0.021 | |
GuHCl Extract | ||||
Collagens | COFA1 | Collagen alpha-1(XV) chain | 0.67 | 0.019 |
ECM glycoproteins | LAMA5 | Laminin subunit alpha-5 | 0.81 | 0.006 |
ECM affiliated proteins | ANXA2 | Annexin A2 | 0.67 | 0.046 |
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Lofaro, F.D.; Cisterna, B.; Lacavalla, M.A.; Boschi, F.; Malatesta, M.; Quaglino, D.; Zancanaro, C.; Boraldi, F. Age-Related Changes in the Matrisome of the Mouse Skeletal Muscle. Int. J. Mol. Sci. 2021, 22, 10564. https://doi.org/10.3390/ijms221910564
Lofaro FD, Cisterna B, Lacavalla MA, Boschi F, Malatesta M, Quaglino D, Zancanaro C, Boraldi F. Age-Related Changes in the Matrisome of the Mouse Skeletal Muscle. International Journal of Molecular Sciences. 2021; 22(19):10564. https://doi.org/10.3390/ijms221910564
Chicago/Turabian StyleLofaro, Francesco Demetrio, Barbara Cisterna, Maria Assunta Lacavalla, Federico Boschi, Manuela Malatesta, Daniela Quaglino, Carlo Zancanaro, and Federica Boraldi. 2021. "Age-Related Changes in the Matrisome of the Mouse Skeletal Muscle" International Journal of Molecular Sciences 22, no. 19: 10564. https://doi.org/10.3390/ijms221910564