Genome-Wide Analysis of WRKY Gene Family and the Dynamic Responses of Key WRKY Genes Involved in Ostrinia furnacalis Attack in Zea mays
Abstract
:1. Introduction
2. Results
2.1. Identification and Characterization of WRKY Genes in Maize
2.2. Classification, Phylogenetic Analysis, and Motif Composition of the ZmWRKY Genes
2.3. Chromosomal Location, Gene Duplication, and Collinearity Analysis of ZmWRKYs
2.4. Expression of ZmWRKY Genes in Response to O. furnacalis Feeding
2.5. Prediction of Transcriptional Regulation and Differential Expression Analysis in Key ZmWRKY Genes
3. Discussion
4. Materials and Methods
4.1. Identification and Sequence Analysis of WRKY Genes in Maize
4.2. Sequence Alignment, Phylogenetic and Conserved Motifs Analysis
4.3. Chromosomal Location, Gene Duplication, and Synteny Analysis
4.4. RNA-Seq Analysis
4.5. Transcriptional Regulation Prediction and Functional Annotation
4.6. Plant Materials and qPCR Validation
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tang, Y.; Guo, J.; Zhang, T.; Bai, S.; He, K.; Wang, Z. Genome-Wide Analysis of WRKY Gene Family and the Dynamic Responses of Key WRKY Genes Involved in Ostrinia furnacalis Attack in Zea mays. Int. J. Mol. Sci. 2021, 22, 13045. https://doi.org/10.3390/ijms222313045
Tang Y, Guo J, Zhang T, Bai S, He K, Wang Z. Genome-Wide Analysis of WRKY Gene Family and the Dynamic Responses of Key WRKY Genes Involved in Ostrinia furnacalis Attack in Zea mays. International Journal of Molecular Sciences. 2021; 22(23):13045. https://doi.org/10.3390/ijms222313045
Chicago/Turabian StyleTang, Yin, Jingfei Guo, Tiantao Zhang, Shuxiong Bai, Kanglai He, and Zhenying Wang. 2021. "Genome-Wide Analysis of WRKY Gene Family and the Dynamic Responses of Key WRKY Genes Involved in Ostrinia furnacalis Attack in Zea mays" International Journal of Molecular Sciences 22, no. 23: 13045. https://doi.org/10.3390/ijms222313045