Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B
Abstract
:1. Introduction
2. Results
2.1. Development of a NanoBiT Assay to Monitor the Interaction of Human GGTase-I and Rap1B
2.2. Effect of the Competitive CAAX Peptidomimetic GGTase-I Inhibitor N-[4-[2(R)-amino-3-mercaptopropyl]amino-2-(1-naphthalenylbenzoyl]-L-leucine methyl ester trifluoroacetate salt (GGTI-298) on the Interaction of GGTase-I and Rap1B and the Formation of A2AAR Homodimers
2.3. Interaction of GGTase-I with Prenylation-Deficient Rap1B Mutants C181G, C181S, and ΔCQLL
2.4. Effect of A2BAR Agonists and Antagonists on the Interaction of GGTase-I and Rap1B WT
2.5. Effect of A2AAR Agonists and Antagonists on the Interaction of GGTase-I and Rap1B
3. Discussion
4. Materials and Methods
4.1. Material
4.2. Expression Vectors and Molecular Cloning
- f-Rap1B WT-Sgf1: 5′-ggacGCGATCGCCCGTGAGTATAAGCTAGTCGTTC
- r-Rap1B WT-PmeI: 5′-gtccGTTTAAACTTAAAGCAGCTGACATGATGAC
- r-Rap1B-(C181G)-PmeI: 5′-gtccGTTTAAACTTAAAGCAGCTGACCTGATGAC
- r-Rap1B-(C181S)-PmeI: 5′-gtccGTTTAAACTTAAAGCAGCTGACTTGATGAC
- r-Rap1B-(ΔCQLL)-PmeI: 5′-gtccGTTTAAACTTATGATGACTTTTTGCGAGCC
- f-GGTase-I-β-Sgf1: 5′-ggacGCGATCGCCGCGGCCACTGAGGATGAGAG
- r-GGTase-I-β-PmeI: 5′-gtccGTTTAAACTCATGTGGAGATATGTACATTCTC
- f-FTase α-Sgf1: 5′-ggacGCGATCGCCGCGGCCACCGAGGG
- r-FTase α-PmeI: 5′-gtccGTTTAAACTTATTGCTGTACATTTGTTGGTG.
- pFN217k LgBiT→forward: 5′ GGAACGGCAACAAAATTATCGAC
- pFN217k LgBiT→reverse: 5′ CATGCCTGCAGGTCGACTCTAG
- pFN218k SmBiT→forward: 5′ CAGTTCAATTACAGCTCTTAAGGCTAGAG
- pFN218k SmBiT→reverse: 5′ CAGCTTGCATGCCTGCAG
- f-hA2A-SgfI: 5′-ggacGCGATCGCCATGCCCATCATGGGCTCCTC
- r-hA2A-PmeI: 5′-gtccGTTTAAACGGACACTCCTGCTCCATCCTG
- pFC219K LgBiT→forward: 5′ CTGGTTTAGTGAACCGTCAGATC
- pFC219K LgBiT→reverse: 5′ GCAATACGTCGACGTTATCAGCTG
- pFC220K SmBiT→forward: 5′ CAGTTCAATTACAGCTCTTAAGGCTAGAG
- pFC220K SmBiT→reverse: 5′ CAGCTTGCATGCCTGCAG
- f-: TTTGAATTCATGGCGGCCACCGAG
- r-: AAAGGATCCTTATTGCTGTACATTTGTTGG.
4.3. Cell Culture
4.4. Transient Transfection of HEK293 Cells Stably Expressing the Human A2BAR
4.5. NanoBiT Assays
4.6. Treatment with the Cell-Permeable and Selective GGTase-I Inhibitor GGTI-298
4.7. Treatment with Adenosine A2A- and A2B-Receptor Agonists and Antagonists
4.8. Transient Transfection of HEK-A2B Cells for Western Blot Analysis
4.9. Preparation of Cytosolic Extract and Whole Cell Lysate of Transfected HEK-A2B Cells
4.10. Bradford Protein Determination
4.11. SDS-Page and Western Blotting
4.12. cAMP Accumulation Experiments
4.13. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
A2AAR | adenosine A2A receptor |
A2BAR | adenosine A2B receptor |
ADA | adenosine deaminase |
AR | adenosine receptor |
BAY60-6583 | [2-((6-amino-3,5-dicyano-4-[4-(cyclopropylmethoxy)phenyl]pyridin-2-yl)sulfanyl)acetamide] |
BiFC | bimolecular fluorescence complementation |
BSA | bovine serum albumin |
cAMP | cyclic adenosine monophosphate |
CGS-21680 | 2-[p-(2-carboxyethyl)phenylethyl-amino]-5′-N-ethylcarboxamido adenosine |
DMEM | Dulbecco’s Modified Eagle Medium |
DMSO | dimethyl sulfoxide |
FCS | fetal calf serum |
FPP | farnesyl diphosphate |
FTase | farnesyltransferase |
G418 | geneticin |
GAPDH | glyceraldehyde 3-phosphate dehydrogenase |
GGPP | geranylgeranyl diphosphate |
GGTase-I | geranylgeranyltransferase type-I |
GGTase-II | geranylgeranyltransferase type II |
GGTase-III | geranylgeranyltransferase type III |
GGTI-298 | N-[4-[2(R)-amino-3-mercaptopropyl]amino-2-(1-naphthalenylbenzoyl]-L-leucine methyl ester trifluoroacetate salt |
GPCR | G protein-coupled receptor |
HA | hemagglutinin |
HEK | human embryonic kidney |
IC50 | half maximal inhibitory concentration |
MSX-2 | (3-(3-hydroxypropyl)-7-methyl-8-(m-methoxystyryl)-1-propargylxanthine) |
NECA | 5′-N-ethylcarboxamidoadenosine |
PBR | polybasic region |
PKA | protein kinase A |
PS | penicillin-streptomycin |
PSB-603 | 8-(4-(4-(4-chlorophenyl)piperazine-1-sulfonyl)phenyl)-1-propylxanthine |
SDS-PAGE | sodium dodecyl sulfate gel electrophoresis |
TBS | Tris-buffered saline |
WT | wild-type |
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Hinz, S.; Jung, D.; Hauert, D.; Bachmann, H.S. Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B. Int. J. Mol. Sci. 2021, 22, 2501. https://doi.org/10.3390/ijms22052501
Hinz S, Jung D, Hauert D, Bachmann HS. Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B. International Journal of Molecular Sciences. 2021; 22(5):2501. https://doi.org/10.3390/ijms22052501
Chicago/Turabian StyleHinz, Sonja, Dominik Jung, Dorota Hauert, and Hagen S. Bachmann. 2021. "Molecular and Pharmacological Characterization of the Interaction between Human Geranylgeranyltransferase Type I and Ras-Related Protein Rap1B" International Journal of Molecular Sciences 22, no. 5: 2501. https://doi.org/10.3390/ijms22052501