A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells
Abstract
:1. Introduction
2. Results
2.1. Strongly Increased Migratory Potential of Primary hPDLCs, hOFs, and HUVECs toward the Volume-Stable Collagen Matrix
2.2. Significantly Increased Adhesive Properties of Primary hPDLCs, hOFs, and HUVECs on the Volume-Stable Collagen Matrix
2.3. Adsorption and Release of Recombinant Growth Factors from the Volume-Stable Collagen Matrix over a 12-Day Period
2.4. Release Kinetics of Growth Factors from the Volume-Stable Collagen Matrix
2.5. Increased Antifibrinolytic and Inhibited Profibrinolytic Gene Expression in Primary hPDLCs, hOFs, and HUVECs Grown on the Native/Uncoated Volume-Stable Collagen Matrix
2.6. Growth Factors Applied in Suspension or as a Coating on the Volume-Stable Collagen Matrix Exhibit Cell Type-Dependent Effects on Anti- and Pro-fibrinolytic Gene Expression. Potential Contribution of Growth factors to the Equilibrium between Coagulation and Fibrinolysis
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. Cell Migration Assay
4.3. Cell Adhesion Assay
4.4. Adsorption and Release of Growth Factors from Volume-Stable Collagen Matrix
4.5. Gene Expression Analyses by qRT-PCR
4.6. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Symbol | Gene Bank Accession Number | Primer Pair (fwd/rev) | Amplicon Size (bp) |
---|---|---|---|
THBD | NM_000361 | 5′- TCCTCTGCGAGTTCCACTTC-3’ 5′- GCCGTAGGTGATCGAGACG-3’ | 89 |
SERPINE1 | NM_001165413 | 5′- AGTGGACTTTTCAGAGGTGGA-3’ 5′- GCCGTTGAAGTAGAGGGCATT-3’ | 151 |
THBS1 | NM_003246.3 | 5′- ATCCGCAAAGTGACTGAAGAG-3’ 5′- GCAGATGGTAACTGAGTTCTGAC-3’ | 152 |
F3 | NM_001993 | 5′- TAAGCACTAAGTCAGGAGATTGG-3’ 5′- TGTCCGAGGTTTGTCTCCAG-3’ | 216 |
PLAT | NM_000930 | 5′- AAACCCAGATCGAGACTCAAAGC-3’ 5′- GGTAGGCTGACCCATTCCC-3’ | 131 |
PLAU | NM_001145031 | 5′- GGGAATGGTCACTTTTACCGAG-3’ 5′- GGGCATGGTACGTTTGCTG-3’ | 103 |
PLAUR | NM_001005377 | 5′- GAGCTATCGGACTGGCTTGAA-3’ 5′- CGGCTTCGGGAATAGGTGAC-3’ | 108 |
MMP14 | NM_004995.3 | 5′- TCAAAGGAGACAAGCATTGGG-3’ 5′- CGGTAGTACTTGTTTCCACGG-3’ | 165 |
GAPDH | NM_001256799.2 | 5′- ATCAAGAAGGTGGTGAAGCAG-3’ 5′- TCGTTGTCATACCAGGAAATGAG-3’ | 178 |
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Asparuhova, M.B.; Stähli, A.; Guldener, K.; Sculean, A. A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells. Int. J. Mol. Sci. 2021, 22, 4051. https://doi.org/10.3390/ijms22084051
Asparuhova MB, Stähli A, Guldener K, Sculean A. A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells. International Journal of Molecular Sciences. 2021; 22(8):4051. https://doi.org/10.3390/ijms22084051
Chicago/Turabian StyleAsparuhova, Maria B., Alexandra Stähli, Kevin Guldener, and Anton Sculean. 2021. "A Novel Volume-Stable Collagen Matrix Induces Changes in the Behavior of Primary Human Oral Fibroblasts, Periodontal Ligament, and Endothelial Cells" International Journal of Molecular Sciences 22, no. 8: 4051. https://doi.org/10.3390/ijms22084051