Physiological Ovarian Aging Is Associated with Altered Expression of Post-Translational Modifications in Mice
Abstract
:1. Introduction
2. Results
2.1. Ovarian Function of 3M, 12M, and 17M Mice Showed Gradual Decline during the Physiological Aging Process
2.2. Ovarian Aging Is Associated with Altered Ovarian Levels of 14 PTMs and Newly Identified Metabolism-Related Lysine Modifications
2.3. Histone Modification Levels of Kme3 and Ubiqutin, Kpr, and O-GlcNA Were Altered during Ovarian Aging
2.4. The Expression of Regulatory Enzyme Genes including TIP60, P300, PRDM9, KMT5B, and KMT5C Increases during Ovarian Aging
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Animals
5.2. Histological Analysis of Ovarian Tissue and Ovarian Follicle Count
5.3. Quantitative Real-Time PCR
5.4. Western Blot Analysis
5.5. Immunoprecipitation
5.6. Statistical Methods
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wei, M.; Li, J.; Yan, H.; Luo, T.; Huang, J.; Yuan, Y.; Hu, L.; Zheng, L. Physiological Ovarian Aging Is Associated with Altered Expression of Post-Translational Modifications in Mice. Int. J. Mol. Sci. 2022, 23, 2. https://doi.org/10.3390/ijms23010002
Wei M, Li J, Yan H, Luo T, Huang J, Yuan Y, Hu L, Zheng L. Physiological Ovarian Aging Is Associated with Altered Expression of Post-Translational Modifications in Mice. International Journal of Molecular Sciences. 2022; 23(1):2. https://doi.org/10.3390/ijms23010002
Chicago/Turabian StyleWei, Minli, Jia Li, Huilin Yan, Tao Luo, Jiang Huang, Yangyang Yuan, Liaoliao Hu, and Liping Zheng. 2022. "Physiological Ovarian Aging Is Associated with Altered Expression of Post-Translational Modifications in Mice" International Journal of Molecular Sciences 23, no. 1: 2. https://doi.org/10.3390/ijms23010002