Lack of Charge Interaction in the Ion Binding Site Determines Anion Selectivity in the Sodium Bicarbonate Cotransporter NBCe1
Abstract
:1. Introduction
2. Results
2.1. ICl Produced by D555E
2.2. Na+ Prerequisite for HCO3− to Access Its Binding Site
2.3. Lack of ICl in the TM5-Replaced Chimeric Transporter
2.4. ICl Induced by Lys558 Replacement in the TM5 Chimeric Transporter
2.5. Salt Bridge between Glu555 and Lys558
2.6. Glu555–Lys558 Charge Interaction
3. Discussion
4. Materials and Methods
4.1. TM5-Replaced Chimeric Transporter and Point Mutants
4.2. Protein Expression in Xenopus Oocytes
4.3. Two-Electrode Voltage Clamp
4.4. Measurement of Intracellular pH (pHi)
4.5. Salt Bridge Experiment
4.6. Analysis of Charge Interaction in Site S2
4.7. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lee, S.; Lin, J.; Choi, I. Lack of Charge Interaction in the Ion Binding Site Determines Anion Selectivity in the Sodium Bicarbonate Cotransporter NBCe1. Int. J. Mol. Sci. 2022, 23, 532. https://doi.org/10.3390/ijms23010532
Lee S, Lin J, Choi I. Lack of Charge Interaction in the Ion Binding Site Determines Anion Selectivity in the Sodium Bicarbonate Cotransporter NBCe1. International Journal of Molecular Sciences. 2022; 23(1):532. https://doi.org/10.3390/ijms23010532
Chicago/Turabian StyleLee, Soojung, Jason Lin, and Inyeong Choi. 2022. "Lack of Charge Interaction in the Ion Binding Site Determines Anion Selectivity in the Sodium Bicarbonate Cotransporter NBCe1" International Journal of Molecular Sciences 23, no. 1: 532. https://doi.org/10.3390/ijms23010532