Genome-Wide Identification, Expression Patterns and Sugar Transport of the Physic Nut SWEET Gene Family and a Functional Analysis of JcSWEET16 in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Identification and Phylogenetic Analysis of SWEET Family Genes in Physic Nut
2.2. Gene Structure and Chromosomal Distribution Analysis of SWEET Family Genes in Physic Nut
2.3. Expression Profiles of SWEET Genes in Different Physic Nut Tissues
2.4. Expression Profiles of JcSWEET Genes under Drought and Salinity Stress
2.5. Transport Activity of JcSWEETs in Yeast
2.6. Overexpression of JcSWEET16 Causes Early Flowering and Increases Salt Stress Tolerance in A. thaliana
3. Discussion
4. Materials and Methods
4.1. Preparation of Plant Materials
4.2. Sequence Database Searches and Gene Cloning
4.3. Sequence Analysis and Phylogenetic Tree Construction
4.4. RNA Isolation and qRT-PCR
4.5. Plasmid Construction and Complementation Assays in Yeast
4.6. Subcellular Localization of JcSWEET16
4.7. Plant Transformation and Salinity Treatment of Transgenic A. thaliana
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wu, Y.; Wu, P.; Xu, S.; Chen, Y.; Li, M.; Wu, G.; Jiang, H. Genome-Wide Identification, Expression Patterns and Sugar Transport of the Physic Nut SWEET Gene Family and a Functional Analysis of JcSWEET16 in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 5391. https://doi.org/10.3390/ijms23105391
Wu Y, Wu P, Xu S, Chen Y, Li M, Wu G, Jiang H. Genome-Wide Identification, Expression Patterns and Sugar Transport of the Physic Nut SWEET Gene Family and a Functional Analysis of JcSWEET16 in Arabidopsis. International Journal of Molecular Sciences. 2022; 23(10):5391. https://doi.org/10.3390/ijms23105391
Chicago/Turabian StyleWu, Youting, Pingzhi Wu, Shaoming Xu, Yaping Chen, Meiru Li, Guojiang Wu, and Huawu Jiang. 2022. "Genome-Wide Identification, Expression Patterns and Sugar Transport of the Physic Nut SWEET Gene Family and a Functional Analysis of JcSWEET16 in Arabidopsis" International Journal of Molecular Sciences 23, no. 10: 5391. https://doi.org/10.3390/ijms23105391