An Integrated Approach Reveals DNA Damage and Proteotoxic Stress as Main Effects of Proton Radiation in S. cerevisiae
Abstract
:1. Introduction
2. Results
2.1. Survival of S. cerevisiae Is Independent of the Radiation Type
2.2. Transcriptomic Response in S. cerevisiae Is Dependent on Radiation Type
2.3. DNA Repair Determines the Survival of S. cerevisiae after PRT and XRT
2.4. PRT Induces DNA Damage and Protein Damage in S. cerevisiae
3. Discussion
4. Materials and Methods
4.1. Strains and Media
4.2. Irradiations
4.3. Plating Assays
4.4. RNA Sequencing and Analysis
4.5. Barcode Sequencing and Analysis
4.6. Fluorescence Microscopy
4.7. Cell Cycle Analysis
4.8. HAC1 mRNA Splicing Detection
4.9. Spot Assays with MG-132
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Vanderwaeren, L.; Dok, R.; Voordeckers, K.; Vandemaele, L.; Verstrepen, K.J.; Nuyts, S. An Integrated Approach Reveals DNA Damage and Proteotoxic Stress as Main Effects of Proton Radiation in S. cerevisiae. Int. J. Mol. Sci. 2022, 23, 5493. https://doi.org/10.3390/ijms23105493
Vanderwaeren L, Dok R, Voordeckers K, Vandemaele L, Verstrepen KJ, Nuyts S. An Integrated Approach Reveals DNA Damage and Proteotoxic Stress as Main Effects of Proton Radiation in S. cerevisiae. International Journal of Molecular Sciences. 2022; 23(10):5493. https://doi.org/10.3390/ijms23105493
Chicago/Turabian StyleVanderwaeren, Laura, Rüveyda Dok, Karin Voordeckers, Laura Vandemaele, Kevin J. Verstrepen, and Sandra Nuyts. 2022. "An Integrated Approach Reveals DNA Damage and Proteotoxic Stress as Main Effects of Proton Radiation in S. cerevisiae" International Journal of Molecular Sciences 23, no. 10: 5493. https://doi.org/10.3390/ijms23105493