Integration of Eukaryotic Energy Metabolism: The Intramitochondrial and Cytosolic Energy States ([ATP]f/[ADP]f[Pi])
Abstract
:1. Introduction
Intramitochondrial Metabolism in Eukaryotes
2. Results and Discussion
2.1. The Intramitochondrial [ATP]/[ADP][Pi]: A. In Vitro Measurement of Nucleotide Concentrations
2.2. The Intramitochondrial [ATP]/[ADP][Pi]: B. the Effect of pH and Membrane Potential on Transport of ATP and ADP across the Inner Membrane
2.3. In Vivo Measurements of Cytoplasmic [ATP]/[ADP]: The Difference between Cytoplasmic [ATP]T/[ADP]T and [ATP]f/[ADP]f
2.4. The Intramitochondrial Energy State ([ATP]/[ADP][Pi]) Inferred by Metabolic Reactions Localized to the Matrix: A. Nucleoside Kinases
2.5. The Intramitochondrial Energy State ([ATP]/[ADP][Pi]) Inferred by Metabolic Reactions Localized to the Matrix: B. Phosphoenoylpyruvate Carboxykinase
2.6. The Intramitochondrial Energy State ([ATP]/[ADP][Pi]) Inferred by Metabolic Reactions Localized to the Matrix: C. Succinate Thiokinase (Succinate CoA Ligase)
2.7. The Intramitochondrial Energy State Inferred by Metabolic REACTIONS Localized to the Matrix: D. Acetate-CoA Ligase)
3. Overview
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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[CoASH]/[succCoA] * | [ATP]/[ADP] External # | O2 rate | [GTP]f/[GDP]f[Pi] in the Matrix * | [GTP]f/[GDP]f in the Matrix * |
---|---|---|---|---|
<0.01 | 43 | 0.048 | >3.33 × 105 M−1 | >333 |
0.011 | 40 | 0.064 | 3.0 × 105 M−1 | 300 |
0.057 | 25 | 0.117 | 3.3 × 104 M−1 | 33 |
0.33 | 2 | 0.171 | 1.0 × 104 M−1 | 10 |
[Acetyl-CoA]/ [HSCoA] | [Acetate] | Mass Action Ratio [ATP]/[ADP][Pi] = 5 × 104 | Mass Action Ratio [ATP]/[ADP][Pi] = 1 × 102 | |
---|---|---|---|---|
Table V * | 2 | 2 × 10−4 | 0.2 | 100 |
Table VII * | 2.8 | 2 × 10−4 | 0.28 | 140 |
Table V * | 2 | 2 × 10−3 | 0.02 | 10 |
Table VII * | 2.8 | 2 × 10−3 | 0.028 | 14 |
Figure 8 * | 1 | 2 × 10−4 | 0.1 | 50 |
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Wilson, D.F.; Matschinsky, F.M. Integration of Eukaryotic Energy Metabolism: The Intramitochondrial and Cytosolic Energy States ([ATP]f/[ADP]f[Pi]). Int. J. Mol. Sci. 2022, 23, 5550. https://doi.org/10.3390/ijms23105550
Wilson DF, Matschinsky FM. Integration of Eukaryotic Energy Metabolism: The Intramitochondrial and Cytosolic Energy States ([ATP]f/[ADP]f[Pi]). International Journal of Molecular Sciences. 2022; 23(10):5550. https://doi.org/10.3390/ijms23105550
Chicago/Turabian StyleWilson, David F., and Franz M. Matschinsky. 2022. "Integration of Eukaryotic Energy Metabolism: The Intramitochondrial and Cytosolic Energy States ([ATP]f/[ADP]f[Pi])" International Journal of Molecular Sciences 23, no. 10: 5550. https://doi.org/10.3390/ijms23105550