The Increase of miR-195-5p Reduces Intestinal Permeability in Ulcerative Colitis, Modulating Tight Junctions’ Expression
Abstract
:1. Introduction
2. Results
2.1. Effect of miR-195-5p Increase after TNF-α Stimulation
2.2. miR-195-5p Modulates the Structural Organization of TJ Altered by TNF-α
2.3. miR-195-5p Influences the Intestinal Barrier Function
2.4. miR-195-5p Attenuates the Intestinal Inflammatory Response in DSS-Induced Colitis
3. Discussion
4. Materials and Methods
4.1. Animal Experiments
4.2. Cell Cultures and In Vitro Transfection
4.3. RNA Extraction and Real-Time PCR
4.4. Western Blot
4.5. Immunofluorescence
4.6. Permeability Assay
4.7. DSS-Induce Mice Colitis Models and In Vivo Transfection
4.8. Histology
4.9. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
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Scalavino, V.; Piccinno, E.; Bianco, G.; Schena, N.; Armentano, R.; Giannelli, G.; Serino, G. The Increase of miR-195-5p Reduces Intestinal Permeability in Ulcerative Colitis, Modulating Tight Junctions’ Expression. Int. J. Mol. Sci. 2022, 23, 5840. https://doi.org/10.3390/ijms23105840
Scalavino V, Piccinno E, Bianco G, Schena N, Armentano R, Giannelli G, Serino G. The Increase of miR-195-5p Reduces Intestinal Permeability in Ulcerative Colitis, Modulating Tight Junctions’ Expression. International Journal of Molecular Sciences. 2022; 23(10):5840. https://doi.org/10.3390/ijms23105840
Chicago/Turabian StyleScalavino, Viviana, Emanuele Piccinno, Giusy Bianco, Nicolò Schena, Raffaele Armentano, Gianluigi Giannelli, and Grazia Serino. 2022. "The Increase of miR-195-5p Reduces Intestinal Permeability in Ulcerative Colitis, Modulating Tight Junctions’ Expression" International Journal of Molecular Sciences 23, no. 10: 5840. https://doi.org/10.3390/ijms23105840