Central Kisspeptin Does Not Affect ERK1/2 or p38 Phosphorylation in Oxytocin Neurons of Late-Pregnant Rats
Abstract
:1. Introduction
2. Results
2.1. Kisspeptin Does Not Affect pERK1/2 Expression in SON or PVN Oxytocin Neurons
2.2. Kisspeptin Does Not Affect p-p38 Expression in SON or PVN Oxytocin Neurons
2.3. Kisspeptin Does Not Affect pERK1/2 or p-p38 Expression in Brainstem Noradrenergic Neurons in the NTS, RVLM or LC
2.4. Kisspeptin Does Not Affect pERK1/2 Expression in the DRN Neurons
2.5. Kisspeptin Does Not Affect pERK1/2 or p-p38 Expression in the OVLT, MnPO or SFO
2.6. Kisspeptin Does Not Affect pERK1/2 or p-p38 Expression in the AVPe, PeN or pERK1/2 Expression in the ARC
3. Discussion
3.1. Local Kisspeptin Activation of Oxytocin Neurons in Late Pregnancy
3.2. Lack of Kisspeptin Induction of ERK1/2 or p38 Phosphorylation in Afferent Inputs to the Oxytocin System
4. Materials and Methods
4.1. Ethical Approval
4.2. Animals
4.3. Intracerebroventricular Cannulation and Kisspeptin Administration
4.4. Brain Collection and Sectioning
4.5. Immunohistochemistry
4.6. Statistical Analysis
4.7. Concluding Remarks
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
aCSF | artificial cerebrospinal fluid |
ARC | arcuate nucleus |
AVPe | anteroventral periventricular nucleus |
ERK1/2 | extracellular regulated kinase 1/2 |
ICV | intracerebroventricular |
Kiss1R | kisspeptin-1 receptor |
MnPO | median preoptic nucleus |
OVLT | organum vasculosum lamina terminalis |
NPFFR | Neuropeptide FF receptor |
pERK1/2 | phosphorylated ERK1/2 |
p-p38 | phosphorylated p38 |
SFO | subfornical organ |
SON | supraoptic nucleus |
PVN | paraventricular nucleus |
PeN | periventricular nucleus |
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Abbasi, M.; Augustine, R.A.; Iremonger, K.J.; Brown, C.H. Central Kisspeptin Does Not Affect ERK1/2 or p38 Phosphorylation in Oxytocin Neurons of Late-Pregnant Rats. Int. J. Mol. Sci. 2022, 23, 7729. https://doi.org/10.3390/ijms23147729
Abbasi M, Augustine RA, Iremonger KJ, Brown CH. Central Kisspeptin Does Not Affect ERK1/2 or p38 Phosphorylation in Oxytocin Neurons of Late-Pregnant Rats. International Journal of Molecular Sciences. 2022; 23(14):7729. https://doi.org/10.3390/ijms23147729
Chicago/Turabian StyleAbbasi, Mehwish, Rachael A. Augustine, Karl J. Iremonger, and Colin H. Brown. 2022. "Central Kisspeptin Does Not Affect ERK1/2 or p38 Phosphorylation in Oxytocin Neurons of Late-Pregnant Rats" International Journal of Molecular Sciences 23, no. 14: 7729. https://doi.org/10.3390/ijms23147729