Oxidation Stress as a Mechanism of Aging in Human Erythrocytes: Protective Effect of Quercetin
Abstract
:1. Introduction
2. Results
2.1. Thiobarbituric Acid Reactive Substances (TBARS) Levels: Effective Concentration of Q
2.2. SO42− Uptake Measurement
2.3. Thiobarbituric Acid Reactive Substances (TBARS) Levels
2.4. Total Sulfhydryl Group Content Measurement
2.5. Glycated Hemoglobin Levels
2.6. GSH/GSSG Ratio Measurement
3. Discussion
4. Materials and Methods
4.1. Solution and Chemical Products
4.2. Erythrocyte Preparation
4.3. Thiobarbituric Acid Reactive Substances (TBARS) Level Measurements
4.4. SO42− Uptake Measurement
4.4.1. Control Condition
4.4.2. Experimental Conditions
4.5. Total Sulfhydryl Group (-SH) Content Measurement
4.6. Measurement of Glycated Hemoglobin (%A1c)
4.7. Measurement of GSH/GSSG Ratio
4.8. Experimental Data and Statistics
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Experimental Conditions | Rate Constant (min−1) | Time (min) | n | SO42− Amount Trapped after 45 min of Incubation in SO42− Medium [SO42−] L Cells × 10−2 |
---|---|---|---|---|
Untreated (control) | 0.064 ± 0.001 | 15.47 | 10 | 379.60 ± 18.63 |
10 µM Q | 0.063 ± 0.002 ns | 15.64 | 10 | 388.35 ± 19.90 ns |
100 mM d-Gal | 0.106 ± 0.001 *** | 9.36 | 7 | 524.35 ± 16.50 *** |
10 µM Q + 100 mM d-Gal | 0.080 ± 0.001 §§§ | 12.32 | 5 | 349.97 ± 11.15 §§§ |
10 µM DIDS | 0.016 ± 0.001 *** | 62.50 | 5 | 5.49 ± 3.50 *** |
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Remigante, A.; Spinelli, S.; Basile, N.; Caruso, D.; Falliti, G.; Dossena, S.; Marino, A.; Morabito, R. Oxidation Stress as a Mechanism of Aging in Human Erythrocytes: Protective Effect of Quercetin. Int. J. Mol. Sci. 2022, 23, 7781. https://doi.org/10.3390/ijms23147781
Remigante A, Spinelli S, Basile N, Caruso D, Falliti G, Dossena S, Marino A, Morabito R. Oxidation Stress as a Mechanism of Aging in Human Erythrocytes: Protective Effect of Quercetin. International Journal of Molecular Sciences. 2022; 23(14):7781. https://doi.org/10.3390/ijms23147781
Chicago/Turabian StyleRemigante, Alessia, Sara Spinelli, Nancy Basile, Daniele Caruso, Giuseppe Falliti, Silvia Dossena, Angela Marino, and Rossana Morabito. 2022. "Oxidation Stress as a Mechanism of Aging in Human Erythrocytes: Protective Effect of Quercetin" International Journal of Molecular Sciences 23, no. 14: 7781. https://doi.org/10.3390/ijms23147781