Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine
Abstract
:1. Introduction
2. Results
2.1. Altered O-GlcNAc Levels Affect the Expression of OGT and OGA in Bovine GCs
2.2. Disruption of O-GlcNAc Cycling Affects Viability and Proliferation of GCs
2.3. Disruption of O-GlcNAc Cycling Induces Cell Apoptosis in Bovine GCs
2.4. Perturbation of O-GlcNAc Cycling in Bovine GCs Reduces Glycolysis
2.5. Alteration in O-GlcNAc Levels Impairs Mitochondria Homeostasis, ATP Production, and the Tricarboxylic Acid (TCA) Cycle
2.6. Disruption of O-GlcNAc Cycling Changes the Expression of Thioredoxin-Interacting Protein (TXNIP)
3. Discussion
4. Materials and Methods
4.1. GC Isolation and Culture
4.2. RNA Extraction and Quantitative Real-Time PCR (qRT-PCR)
4.3. Protein Extraction and Western Blot Analysis
4.4. Cell Viability Analysis
4.5. Apoptotic Assay
4.6. Assessment of MMP
4.7. Enzymatic Activity Assay
4.8. Metabolites Measurement and Intracellular ATP Level Assays
4.9. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer | Sequence | Accession No. |
---|---|---|---|
GAPDH | Forward | 5′-GGGTCATCATCTCTGCACCT-3′ | NM_001034034 |
Reverse | 5′-GGTCATAAGTCCCTCCACGA-3′ | ||
OGT | Forward | 5′-AGGGTTCGAAGGCTGTAACTG-3′ | NM_001098070 |
Reverse | 5′-ACTCAGCTAACCCTGTGCTG-3′ | ||
OGA | Forward | 5′-TTGAAGAATGGCGGTCACGA-3′ | NM_001206448 |
Reverse | 5′-TGACTACGACACCCTAACCAC-3′ | ||
CDC42 | Forward | 5′-GTTGTTGTGGGTGATGGTGC-3′ | NM_001046332 |
Reverse | 5′-TCCCCACCAATCATAACTGT-3′ | ||
PCNA | Forward | 5′-GTCCAGGGCTCCATCTTGAA-3′ | NM_001034494 |
Reverse | 5′-CAAGGAGACATGAGACGAGT-3′ | ||
CCND2 | Forward | 5′-TGACCGCTGAGAAGTTATGC-3′ | NM_001076372 |
Reverse | 5′-CGCCAGGTTCCATTTCAACT-3′ | ||
BAX | Forward | 5′-GGCTGGACATTGGACTTCCTTC-3′ | NM_173894 |
Reverse | 5′-TGGTCACTGTCTGCCATGTGG-3′ | ||
BCL-2 | Forward | 5′-GAGTCGGATCGCAACTTGGA-3′ | NM_001077486 |
Reverse | 5′-CTCTCGGCTGCTGCATTGT-3′ | ||
CASPASE-3 | Forward | 5′-TACTTGGGAAGGTGTGAGAAAACTAA-3′ | NM_001077840 |
Reverse | 5′-AACCCGTCTCCCTTTATATTGCT-3′ | ||
TXNIP | Forward | 5′-TGGACTACTGGGTGAAGGCT-3′ | NM_001101875 |
Reverse | 5′-AGCTGACACAGGTTCCAGTAAAT-3′ |
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Wang, T.-F.; Feng, Z.-Q.; Sun, Y.-W.; Zhao, S.-J.; Zou, H.-Y.; Hao, H.-S.; Du, W.-H.; Zhao, X.-M.; Zhu, H.-B.; Pang, Y.-W. Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine. Int. J. Mol. Sci. 2022, 23, 7815. https://doi.org/10.3390/ijms23147815
Wang T-F, Feng Z-Q, Sun Y-W, Zhao S-J, Zou H-Y, Hao H-S, Du W-H, Zhao X-M, Zhu H-B, Pang Y-W. Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine. International Journal of Molecular Sciences. 2022; 23(14):7815. https://doi.org/10.3390/ijms23147815
Chicago/Turabian StyleWang, Teng-Fei, Zhi-Qiang Feng, Ya-Wen Sun, Shan-Jiang Zhao, Hui-Ying Zou, Hai-Sheng Hao, Wei-Hua Du, Xue-Ming Zhao, Hua-Bin Zhu, and Yun-Wei Pang. 2022. "Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine" International Journal of Molecular Sciences 23, no. 14: 7815. https://doi.org/10.3390/ijms23147815