Phospholipase A1 Member A Deficiency Alleviates Mannan-Induced Psoriatic Arthritis in Mice Model
Abstract
:1. Introduction
2. Results
2.1. Phenotypes of Pla1a−/− Mice
2.2. The Mouse Model of MIP
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Model of MIP
4.3. Immune Cell Sorting
4.4. Luminex
4.5. Micro-Computed Tomography (μCT) Image Acquisition
4.6. Statistics
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Paw Lysate (pg/mL, n = 5) | Paw Lysate (pg/mL, n = 5) | Plasma (pg/mL, n = 5) | |||||||
---|---|---|---|---|---|---|---|---|---|
Control Mice | MIP | MIP | |||||||
WT | KO | p Value | WT | KO | p Value | WT | KO | p Value | |
Eotaxin | 72.97 ± 8.85 | 106.22 ± 64.79 | 0.339 | 176.10 ± 27.21 | 132.03 ± 11.59 | 0.018 | 238.52 ± 39.65 | 288.77 ± 26.23 | 0.067 |
G-CSF | 6.60 ± 0.56 | 9.78 ± 3.19 | 0.085 | 152.47 ± 62.87 | 73.30 ± 28.62 | 0.051 | 355.06 ± 66.26 | 295.83 ± 64.08 | 0.235 |
GM-CSF | 11.60 ± 0.88 | 12.13 ± 1.75 | 0.602 | 21.74 ± 1.94 | 26.24 ± 8.30 | 0.322 | 32.60 ± 3.45 | 33.89 ± 2.69 | 0.573 |
IFNγ | 4.11 ± 0.93 | 3.43 ± 0.90 | 0.321 | 2.36 ± 0.31 | 2.81 ± 0.44 | 0.140 | 11.57 ± 4.20 | 15.63 ± 8.90 | 0.433 |
IL-1α | 1298.44 ± 206.51 | 1745.13 ± 567.59 | 0.177 | 1586.86 ± 302.61 | 2259.35 ± 546.18 | 0.063 | 128.78 ± 22.24 | 146.74 ± 26.17 | 0.326 |
IL-1β | 9.66 ± 0.90 | 9.02 ± 1.21 | 0.417 | 23.04 ± 5.52 | 21.38 ± 7.29 | 0.725 | 12.78 ± 0.54 | 16.82 ± 10.01 | 0.443 |
IL-2 | 7.52 ± 2.80 | 5.35 ± 1.97 | 0.241 | 3.55 ± 0.23 | 3.39 ± 0.48 | 0.582 | 29.44 ± 8.83 | 29.93 ± 16.02 | 0.959 |
IL-3 | 1.85 ± 0.11 | 1.79 ± 0.14 | 0.552 | 8.69 ± 11.07 | 3.34 ± 2.41 | 0.372 | 3.11 ± 0.43 | 3.80 ± 1.62 | 0.439 |
IL-4 | 0.54 ± 0.13 | 0.50 ± 0.09 | 0.650 | 0.83 ± 0.58 | 0.64 ± 0.15 | 0.541 | 0.82 ± 0.11 | 1.09 ± 0.35 | 0.179 |
IL-5 | 3.43 ± 1.90 | 3.74 ± 2.06 | 0.828 | 2.13 ± 0.14 | 2.41 ± 0.79 | 0.500 | 7.04 ± 1.53 | 5.97 ± 1.27 | 0.311 |
IL-6 | 6.05 ± 0.36 | 5.58 ± 1.83 | 0.623 | 20.88 ± 5.07 | 14.67 ± 10.82 | 0.329 | 5.35 ± 2.48 | 5.66 ± 2.29 | 0.856 |
IL-7 | 7.92 ± 0.75 | 6.62 ± 0.62 | 0.028 | 7.00 ± 0.75 | 5.61 ± 0.50 | 0.015 | 20.22 ± 11.64 | 9.37 ± 1.32 | 0.101 |
IL-9 | 24.23 ± 1.19 | 22.58 ± 2.01 | 0.196 | 21.11 ± 1.02 | 19.35 ± 1.09 | 0.046 | 83.84 ± 30.47 | 69.96 ± 7.32 | 0.402 |
IL-10 | 26.15 ± 3.70 | 20.18 ± 7.17 | 0.177 | 19.86 ± 8.83 | 13.93 ± 4.41 | 0.264 | 25.12 ± 2.64 | 25.44 ± 6.10 | 0.925 |
IL-12p40 | 44.37 ± 4.56 | 34.16 ± 7.92 | 0.054 | 31.02 ± 6.35 | 29.32 ± 1.87 | 0.620 | 24.97 ± 2.85 | 27.10 ± 2.25 | 0.274 |
IL-12p70 | 16.47 ± 1.41 | 15.87 ± 1.19 | 0.532 | 26.65 ± 13.31 | 19.73 ± 4.60 | 0.354 | 36.88 ± 7.88 | 51.29 ± 9.54 | 0.048 |
IL-13 | 3.49 ± 1.49 | 3.18 ± 0.85 | 0.725 | 2.98 ± 0.81 | 2.11 ± 0.42 | 0.095 | 204.48 ± 37.72 | 217.22 ± 59.07 | 0.725 |
IL-15 | 40.84 ± 6.33 | 46.98 ± 3.24 | 0.123 | 41.18 ± 3.37 | 36.88 ± 3.62 | 0.120 | 228.26 ± 132.86 | 135.21 ± 13.93 | 0.201 |
IL-17 | 1.12 ± 0.17 | 1.12 ± 0.54 | 0.989 | 84.83 ± 33.40 | 26.87 ± 15.76 | 0.014 | 3.83 ± 1.04 | 3.70 ± 1.19 | 0.875 |
IP-10 | 2.41 ± 0.44 | 2.75 ± 0.75 | 0.456 | 7.62 ± 1.20 | 4.33 ± 1.04 | 0.003 | 70.23 ± 25.89 | 51.20 ± 10.47 | 0.210 |
KC | 29.40 ± 4.74 | 35.14 ± 13.30 | 0.440 | 136.06 ± 20.35 | 82.05 ± 29.22 | 0.016 | 25.48 ± 6.42 | 23.39 ± 11.65 | 0.761 |
LIF | 9.62 ± 1.32 | 11.01 ± 0.61 | 0.093 | 25.66 ± 5.20 | 14.76 ± 1.98 | 0.004 | 8.50 ± 10.72 | 2.49 ± 0.80 | 0.296 |
MCP-1 | 78.25 ± 16.37 | 113.02 ± 49.95 | 0.222 | 229.29 ± 129.40 | 139.90 ± 76.34 | 0.268 | 45.41 ± 5.00 | 62.20 ± 41.74 | 0.448 |
M-CSF | 21.42 ± 4.50 | 20.41 ± 3.21 | 0.724 | 16.24 ± 2.10 | 15.91 ± 3.43 | 0.872 | 38.51 ± 2.67 | 36.84 ± 2.66 | 0.403 |
MIG | 5.99 ± 3.50 | 6.83 ± 3.54 | 0.745 | 19.63 ± 8.05 | 12.96 ± 3.61 | 0.169 | 259.55 ± 35.17 | 607.34 ± 371.66 | 0.099 |
MIP-1α | 27.90 ± 1.47 | 28.19 ± 1.88 | 0.810 | 70.12 ± 10.76 | 58.56 ± 12.08 | 0.191 | 136.01 ± 17.20 | 130.96 ± 27.26 | 0.762 |
MIP-1β | 18.18 ± 4.50 | 17.24 ± 3.04 | 0.739 | 44.31 ± 6.53 | 32.14 ± 7.34 | 0.038 | 95.25 ± 10.97 | 101.15 ± 27.23 | 0.698 |
MIP-2 | 180.89 ± 11.94 | 167.39 ± 26.58 | 0.381 | 1049.48 ± 301.44 | 859.24 ± 304.88 | 0.401 | 733.26 ± 3.52 | 735.67 ± 2.42 | 0.292 |
RANTES | 1.14 ± 0.15 | 1.22 ± 0.22 | 0.576 | 2.97 ± 1.24 | 2.21 ± 0.64 | 0.308 | 21.92 ± 3.13 | 26.81 ± 7.59 | 0.268 |
TNF-α | 3.41 ± 0.30 | 3.40 ± 0.60 | 0.991 | 10.90 ± 2.52 | 9.16 ± 2.77 | 0.380 | 18.17 ± 1.28 | 19.83 ± 3.82 | 0.433 |
VEGF | 21.07 ± 1.71 | 24.68 ± 5.73 | 0.261 | 75.54 ± 7.03 | 54.11 ± 13.11 | 0.020 | 1.48 ± 0.41 | 1.37 ± 0.20 | 0.658 |
LIX | 110.40 ± 52.50 | 85.28 ± 65.33 | 0.565 |
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Zhao, Y.; Aoudjit, F.; Bourgoin, S.G. Phospholipase A1 Member A Deficiency Alleviates Mannan-Induced Psoriatic Arthritis in Mice Model. Int. J. Mol. Sci. 2022, 23, 8559. https://doi.org/10.3390/ijms23158559
Zhao Y, Aoudjit F, Bourgoin SG. Phospholipase A1 Member A Deficiency Alleviates Mannan-Induced Psoriatic Arthritis in Mice Model. International Journal of Molecular Sciences. 2022; 23(15):8559. https://doi.org/10.3390/ijms23158559
Chicago/Turabian StyleZhao, Yang, Fawzi Aoudjit, and Sylvain G. Bourgoin. 2022. "Phospholipase A1 Member A Deficiency Alleviates Mannan-Induced Psoriatic Arthritis in Mice Model" International Journal of Molecular Sciences 23, no. 15: 8559. https://doi.org/10.3390/ijms23158559