Subfunctionalization of D27 Isomerase Genes in Saffron
Abstract
:1. Introduction
2. Results
2.1. Isolation and Identification of SL Biosynthetic Gene D27 in Saffron
2.2. Expression Levels in Vegetative Tissues and Saffron Stigmas
2.3. Activity Assays of CsD27 in E. coli and N. benthamiana
3. Discussion
4. Materials and Methods
4.1. Plant Material and Treatments
4.2. Isolation of cDNA Sequences Encoding D27 Enzyme in Saffron
4.3. Phylogenetic Analysis
4.4. DNA Sequencing and Analysis of the DNA and Protein Sequences
4.5. Expression Analysis
4.6. Carotenoid Extraction and HPLC Analysis
4.7. D27 Activity in E. coli Cells
4.8. Transient Expression of CsD27-1 in Nicotiana benthamiana
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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López-Jiménez, A.J.; Morote, L.; Niza, E.; Mondéjar, M.; Rubio-Moraga, Á.; Diretto, G.; Ahrazem, O.; Gómez-Gómez, L. Subfunctionalization of D27 Isomerase Genes in Saffron. Int. J. Mol. Sci. 2022, 23, 10543. https://doi.org/10.3390/ijms231810543
López-Jiménez AJ, Morote L, Niza E, Mondéjar M, Rubio-Moraga Á, Diretto G, Ahrazem O, Gómez-Gómez L. Subfunctionalization of D27 Isomerase Genes in Saffron. International Journal of Molecular Sciences. 2022; 23(18):10543. https://doi.org/10.3390/ijms231810543
Chicago/Turabian StyleLópez-Jiménez, Alberto José, Lucía Morote, Enrique Niza, María Mondéjar, Ángela Rubio-Moraga, Gianfranco Diretto, Oussama Ahrazem, and Lourdes Gómez-Gómez. 2022. "Subfunctionalization of D27 Isomerase Genes in Saffron" International Journal of Molecular Sciences 23, no. 18: 10543. https://doi.org/10.3390/ijms231810543