Correction: Franz et al. Unraveling a Force-Generating Allosteric Pathway of Actomyosin Communication Associated with ADP and Pi Release. Int. J. Mol. Sci. 2021, 22, 104
Table and Figure Legend
Error in Table
Unit | M765wt | M765AA | M765GGG | |
---|---|---|---|---|
ATP binding to myosin 1 | ||||
K1k+2 | (µM−1s−1) | 0.48 ± 0.01 | 0.38 ± 0.01 | 0.38 ± 0.01 |
k−2 | (s−1) | 0.79 ± 0.49 | 0.54 ± 0.24 | 0.43 ± 0.25 |
k+3+k−3 | (s−1) | 42 ± 1 | 61 ± 2 | 106 ± 8 |
ADP binding to myosin 1 | ||||
k−5K6 | (µM−1s−1) | 0.58 ± 0.02 | 0.38 ± 0.02 | 0.49 ± 0.01 |
k+5 | (s−1) | 1.6 ± 0.1 | 1.8 ± 0.1 | 1.9 ± 0.1 |
1/(K5K6) | (µM) | 2.8 ± 0.2 | 4.7 ± 0.4 | 3.9 ± 0.2 |
Rate of Pi release 1 | ||||
k+4 | (s−1) | 0.02 ± 0.01 | n.d. | 0.02 ± 0.01 |
Unit | M765wt | M765AA | M765GGG | |
Actomyosin interactions in the absence and presence of ADP | ||||
k′+A | (µM−1s−1) | 0.64 ± 0.03 | 0.61 ± 0.03 | 0.61 ± 0.03 |
k′-A | (s−1) | 0.003 | 0.004 | 0.005 |
1/K′A1 | (µM) | 0.0057 ± 0.0005 | 0.010 ± 0.009 | 0.011 ± 0.009 |
k′+DA | (µM−1s−1) | 0.12 ± 0.01 | n.d. | 0.18 ± 0.01 |
k′-DA | (s−1) | 0.006 ± 0.001 | n.d. | 0.005 ± 0.001 |
1/K′DA2 | (µM) | 0.063 ± 0.002 | n.d. | 0.024 ± 0.001 |
ATP interactions of actomyosin | ||||
K′1k′+2 | (µM−1s−1) | 0.24 ± 0.01 | 0.32 ± 0.01 | 0.92 ± 0.01 |
k′+2 | (s−1) | 719 ± 22 | 871 ± 80 | 923 ± 19 |
k′−2 | (s−1) | 0.08 ± 0.05 | 0.62 ± 0.05 | 1.48 ± 0.13 |
1/K′1 | (µM) | 3200 ± 130 | 2702 ± 279 | 883 ± 30 |
ADP interactions of actomyosin | ||||
k′−5K′63 | (µM−1s−1) | >1.0 / >0.74 | >1.47 / >2.94 | 4.7 ± 1 / 3.6 ± 1 4 |
1/(K′5K′6) | (µM) | 135 ± 10 5 | 34 ± 4 5 | 3.4 ± 1 6 |
k′+5 | (s−1) | >100 7 | >100 7 | 16 ± 1 8 |
Pi kinetics of actomyosin | ||||
k′+4 | (s−1) | 3.8 ± 1.1 | n.d. | 14.4 ± 1.0 |
k′+4K′DPA | (µM−1s−1) | 0.04 ± 0.01 | n.d. | 0.79 ± 0.08 |
1/K′DPA | (µM) | 94 ± 32 | n.d. | 18 ± 3 |
Weak-to-strong transition | ||||
k′weak-strong | (s−1) | 1.0 ± 0.01 | n.d. | 2.6 ± 0.04 |
Duty ratio | ||||
tstrong/ttotal | % | 3 ± 1 | <4 ± 1 | 49 ± 4 |
Experimental | % | <1 | n.d. | 35 |
Unit | M765wt | M765AA | M765GGG | |
Equilibrium constants | ||||
K′DPA | (µM−1) | 0.01 | 0.01 | 0.05 |
K′4 | (µM−1) | 0.00001 | 0.00001 | 0.00001 |
K′5 | - | 0.5 | 0.74 | 7.14 |
K′6 | (µM−1) | 0.02 | 0.02 | 0.02 |
K′1 | (µM−1) | 0.0003 | 0.004 | 0.001 |
K′2 | - | 8988 | 1405 | 624 |
K′TA | (µM−1) | 0.001 | 0.001 | 0.001 |
K3 | - | 9.5 | 9.2 | 9.6 |
K′3 | - | 95 | 92 | 96 |
Rate constants of forward reactions | ||||
k′+DPA | (µM−1s−1) | 10 | 10 | 50 |
k′+4 | (s−1) | 3.8 | 5.0 | 14.4 |
k′+5 | (s−1) | 235 | 160 | 20 |
k′+6 | (s−1) | 1000 | 1000 | 1000 |
k′+1 | (µM−1s−1) | 100 | 100 | 100 |
k′+2 | (s−1) | 719 | 871 | 923 |
k′+TA | (s−1) | 1000 | 1000 | 1000 |
k+3 | (s−1) | 38 | 55 | 96 |
k′+3 | (s−1) | 38 | 55 | 96 |
Rate constants of backwards reactions | ||||
k′-DPA | (s−1) | 1000 | 1000 | 1000 |
k′−4 | (µM−1s−1) | 0.000038 | 0.00005 | 0.000144 |
k′−5 | (s−1) | 470 | 216 | 2.8 |
k′−6 | (µM−1s−1) | 20 | 20 | 20 |
k′−1 | (s−1) | 333300 | 250000 | 88500 |
k′−2 | (s−1) | 0.08 | 0.62 | 1.48 |
k′-TA | (µM−1s−1) | 1 | 1 | 1 |
k−3 | (s−1) | 4 | 6 | 10 |
k′−3 | (s−1) | 0.4 | 0.6 | 1 |
Text Correction
- Paragraph 8. The incorrect constants “K′5K′6” should be replaced with “1/(K′5K′6)”.
- Paragraph 10. The incorrect constants “K′DPA” should be replaced with “1/K′DPA”. The incorrect constants “K′DPAk′+4” should be replaced with “k′+4K′DPA”.
- Paragraph 11. The incorrect constants “K′DPA” should be replaced with “1/K′DPA”.
Reference
- Franz, P.; Ewert, W.; Preller, M.; Tsiavaliaris, G. Unraveling a Force-Generating Allosteric Pathway of Actomyosin Communication Associated with ADP and Pi Release. Int. J. Mol. Sci. 2021, 22, 104. [Google Scholar] [CrossRef] [PubMed]
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Franz, P.; Ewert, W.; Preller, M.; Tsiavaliaris, G. Correction: Franz et al. Unraveling a Force-Generating Allosteric Pathway of Actomyosin Communication Associated with ADP and Pi Release. Int. J. Mol. Sci. 2021, 22, 104. Int. J. Mol. Sci. 2022, 23, 11121. https://doi.org/10.3390/ijms231911121
Franz P, Ewert W, Preller M, Tsiavaliaris G. Correction: Franz et al. Unraveling a Force-Generating Allosteric Pathway of Actomyosin Communication Associated with ADP and Pi Release. Int. J. Mol. Sci. 2021, 22, 104. International Journal of Molecular Sciences. 2022; 23(19):11121. https://doi.org/10.3390/ijms231911121
Chicago/Turabian StyleFranz, Peter, Wiebke Ewert, Matthias Preller, and Georgios Tsiavaliaris. 2022. "Correction: Franz et al. Unraveling a Force-Generating Allosteric Pathway of Actomyosin Communication Associated with ADP and Pi Release. Int. J. Mol. Sci. 2021, 22, 104" International Journal of Molecular Sciences 23, no. 19: 11121. https://doi.org/10.3390/ijms231911121