bHLH010/089 Transcription Factors Control Pollen Wall Development via Specific Transcriptional and Metabolic Networks in Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. bhlh010 bhlh089 Pollen Exhibited Collapsed Structure and Severely Reduced Germination Rate
2.2. Both the Exine and Intine Developments Were Abnormal in bhlh010 bhlh089
2.3. Metabolomic Comparison of WT and bhlh010 bhlh089 Inflorescences
2.4. bHLH010/089 Regulate the Expression of Genes Involved in Metabolic Pathways
2.5. Screening of Genes Activated by bHLH010 or/and bHLH089
2.6. The CSLB03 Gene Is a Direct Downstream Target of bHLH010
2.7. CSLB03 Gene Is Required for Pollen Wall Development
3. Discussion
3.1. bHLH010 and bHLH089 May Regulate Pollen Wall Formation through the Synthesis of Polysaccharides
3.2. bHLH010 and bHLH089 May Regulate Pollen Wall Formation through Transporters
4. Materials and Methods
4.1. Plant Material
4.2. Generation of probHLH089: β-Glucuronidase (GUS) Transgenic Lines and GUS Staining Assays
4.3. Semithin Section Examination and Pollen Wall Staining
4.4. Scanning Electron Microscopy (SEM)
4.5. Real-Time PCR
4.6. Metabolome Extraction and Detection
4.7. Differential Metabolite Analysis
4.8. Analysis of the bhlh010 bhlh089 Transcriptomic Data
4.9. Combined Metabolome and Transcriptome Analysis
4.10. Dual-Luciferase Reporter Assays
4.11. Electrophoretic Mobility Shift Assay
4.12. In Vitro Pollen Germination Analysis
4.13. Phylogenetic Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lai, Z.; Wang, J.; Peng, S.-Q.; Chang, F. bHLH010/089 Transcription Factors Control Pollen Wall Development via Specific Transcriptional and Metabolic Networks in Arabidopsis thaliana. Int. J. Mol. Sci. 2022, 23, 11683. https://doi.org/10.3390/ijms231911683
Lai Z, Wang J, Peng S-Q, Chang F. bHLH010/089 Transcription Factors Control Pollen Wall Development via Specific Transcriptional and Metabolic Networks in Arabidopsis thaliana. International Journal of Molecular Sciences. 2022; 23(19):11683. https://doi.org/10.3390/ijms231911683
Chicago/Turabian StyleLai, Zesen, Jianzheng Wang, Shi-Qing Peng, and Fang Chang. 2022. "bHLH010/089 Transcription Factors Control Pollen Wall Development via Specific Transcriptional and Metabolic Networks in Arabidopsis thaliana" International Journal of Molecular Sciences 23, no. 19: 11683. https://doi.org/10.3390/ijms231911683