Competitive Sperm-Marked Beetles for Monitoring Approaches in Genetic Biocontrol and Studies in Reproductive Biology
Abstract
:1. Introduction
2. Results
2.1. Generation of Different Tribolium Castaneum Sperm-Marking Lines
2.2. Competitiveness of Sperm-Marking Lines
2.3. Sperm Use in Consecutive Matings
3. Discussion
4. Materials and Methods
4.1. Beetle Culture
4.2. Isolation of the β2t Promoter Region and Cloning of the Transformation Vectors
4.3. Germline Transformation and Strain Establishment
4.4. Competitive Assays
4.5. Microscopy
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Isah, M.D.; Atika, B.; Dippel, S.; Ahmed, H.M.M.; Wimmer, E.A. Competitive Sperm-Marked Beetles for Monitoring Approaches in Genetic Biocontrol and Studies in Reproductive Biology. Int. J. Mol. Sci. 2022, 23, 12594. https://doi.org/10.3390/ijms232012594
Isah MD, Atika B, Dippel S, Ahmed HMM, Wimmer EA. Competitive Sperm-Marked Beetles for Monitoring Approaches in Genetic Biocontrol and Studies in Reproductive Biology. International Journal of Molecular Sciences. 2022; 23(20):12594. https://doi.org/10.3390/ijms232012594
Chicago/Turabian StyleIsah, Musa Dan’azumi, Bibi Atika, Stefan Dippel, Hassan M. M. Ahmed, and Ernst A. Wimmer. 2022. "Competitive Sperm-Marked Beetles for Monitoring Approaches in Genetic Biocontrol and Studies in Reproductive Biology" International Journal of Molecular Sciences 23, no. 20: 12594. https://doi.org/10.3390/ijms232012594