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Article
Peer-Review Record

Extraction, Characterization, and Platelet Inhibitory Effects of Two Polysaccharides from the Cs-4 Fungus

Int. J. Mol. Sci. 2022, 23(20), 12608; https://doi.org/10.3390/ijms232012608
by Yu-Heng Mao 1,2, Feng-Lin Song 3, Yi-Xuan Xu 1,4,5, Ang-Xin Song 6, Zhao-Mei Wang 7, Ming-Zhu Zhao 1,4,5, Fang He 1,4,5, Ze-Zhong Tian 1,4,5 and Yan Yang 1,4,5,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2022, 23(20), 12608; https://doi.org/10.3390/ijms232012608
Submission received: 13 September 2022 / Revised: 6 October 2022 / Accepted: 10 October 2022 / Published: 20 October 2022
(This article belongs to the Special Issue Dietary Supplements for Metabolic Syndrome)

Round 1

Reviewer 1 Report

The manuscript presents large and comprehensive work on characterization of Cs-4 polysaccharides composition and biological activity which is acceptable for publication 

minor comments

1. It would be better to specify which methods were used to determine the protein and polysaccharide contens in isolates, as well as molecular weights of polysaccharides under study

2. The figures quality could be better regarding image resolution

3. Why didn't authors use pure strain for polysaccharides fermentation?

4. Is it possible the Cs-4 polysacchrides are sulfated like fucoidan which is also has platelet aggregation inhibitory activity?

in figure 4 caption the word "platelet" is missing "Figure 4. The effects of Cs-4 EPSp, IPSp, and their gut bacterial metabolites on human activation. (a)..."

Author Response

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Author Response File: Author Response.pdf

Reviewer 2 Report

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Comments for author File: Comments.pdf

Author Response

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Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

see file

Comments for author File: Comments.pdf

Author Response

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Author Response File: Author Response.pdf

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