Trichostatin D as a Novel KLF2 Activator Attenuates TNFα-Induced Endothelial Inflammation
Abstract
:1. Introduction
2. Results
2.1. Isolation and Identification of TSD from Streptomyces sp. CPCC 203909
2.2. TSD Increases KLF2 Activity and mRNA Levels
2.3. TSD Attenuated Monocyte Adhesion to HUVECs and Exhibited Anti-Inflammatory Effects
2.4. Anti-Inflammatory Effects of TSD in TNFα-Induced HUVECs Depend on KLF2
2.5. TSD Exerts Anti-Inflammatory Effects on Endothelial Cells through the KLF2/NLRP3/Caspase-1/IL-1β Signaling Pathway
2.6. TSD Upregulates the Expression of KLF2 by Inhibiting HDAC4, HDAC5, and HDAC7
2.7. Interaction Modes between TSD and HDAC4, HDAC5, or HDAC7
3. Discussion
4. Materials and Methods
4.1. Fermentation of Streptomyces sp. CPCC 203909
4.2. Extraction, Isolation, Purification, and Structure Identification of Trichostatin D
4.3. Cell Culture
4.4. Cell Transfection and Luciferase Assay
4.5. Cell Viability Assay
4.6. RNA Isolation and RT-qPCR
4.7. Western Blot Analysis
4.8. Monocyte Adhesion Assay
4.9. Small Interfering RNA (SiRNA) Assay
4.10. Molecular Modeling Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Lei, L.; Chen, M.; Wang, C.; Jiang, X.; Li, Y.; Wang, W.; Li, S.; Zhao, L.; Sheng, R.; Han, J.; et al. Trichostatin D as a Novel KLF2 Activator Attenuates TNFα-Induced Endothelial Inflammation. Int. J. Mol. Sci. 2022, 23, 13477. https://doi.org/10.3390/ijms232113477
Lei L, Chen M, Wang C, Jiang X, Li Y, Wang W, Li S, Zhao L, Sheng R, Han J, et al. Trichostatin D as a Novel KLF2 Activator Attenuates TNFα-Induced Endothelial Inflammation. International Journal of Molecular Sciences. 2022; 23(21):13477. https://doi.org/10.3390/ijms232113477
Chicago/Turabian StyleLei, Lijuan, Minghua Chen, Chenyin Wang, Xinhai Jiang, Yinghong Li, Weizhi Wang, Shunwang Li, Liping Zhao, Ren Sheng, Jiangxue Han, and et al. 2022. "Trichostatin D as a Novel KLF2 Activator Attenuates TNFα-Induced Endothelial Inflammation" International Journal of Molecular Sciences 23, no. 21: 13477. https://doi.org/10.3390/ijms232113477