Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients
Abstract
:1. Introduction
2. Results
2.1. Cohort Data
2.2. Differentially Expressed Genes (DEGs) and Enrichment Analysis
2.3. Interactome
2.4. lncRNA Interactome
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Patient Cohort and Sample Processing
5.2. SARS-CoV-2 Detection and Quantification
5.3. Severity Biomarker Investigation
5.4. RNA Extraction and Library Preparation
5.5. RNA Sequencing (RNA-seq) Analysis
5.6. Enrichment Analysis
5.7. Interactome Analysis
5.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Laboratory and Clinical Features | Severe (n = 12) | Mild (n = 5) | p-Value |
---|---|---|---|
SARS-Cov-2 E viral load (median) * | 6620 | 13.8 | 0.0059 |
Hospitalization (in days; median) | 14 | NA | - |
Hypertension | (10) 83.3 % | (1) 20.0 % | - |
Diabetes | (9) 75.0 | 0 | - |
Obesity | 0 | 0 | - |
Dyslipidemia | 0 | 0 | - |
Pneumonia | (12) 100% | 0 | - |
Oxygen supplementation | (12) 100% | 0 | - |
SOFA index (median) | 6 | NA | - |
Neurofilament plasma level (median) | 91.80 pg/mL | 14.52 pg/mL | 0.0275 |
Glial fibrillary acid protein plasma level (median) | 346.0 pg/mL | 143.9 pg/mL | 0.0517 |
Ubiquitin carboxy-terminal hydroxylase L1 plasma level (median) | 100.1 pg/mL | 38.83 pg/mL | 0.0133 |
TAU total protein plasma level (median) | 6.668 pg/mL | 2.599 pg/mL | 0.0133 |
Lethality | (6) 50% | 0 | - |
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Cavalcante, L.T.d.F.; da Fonseca, G.C.; Amado Leon, L.A.; Salvio, A.L.; Brustolini, O.J.; Gerber, A.L.; Guimarães, A.P.d.C.; Marques, C.A.B.; Fernandes, R.A.; Ramos Filho, C.H.F.; et al. Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients. Int. J. Mol. Sci. 2022, 23, 13588. https://doi.org/10.3390/ijms232113588
Cavalcante LTdF, da Fonseca GC, Amado Leon LA, Salvio AL, Brustolini OJ, Gerber AL, Guimarães APdC, Marques CAB, Fernandes RA, Ramos Filho CHF, et al. Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients. International Journal of Molecular Sciences. 2022; 23(21):13588. https://doi.org/10.3390/ijms232113588
Chicago/Turabian StyleCavalcante, Liliane Tavares de Faria, Guilherme Cordenonsi da Fonseca, Luciane Almeida Amado Leon, Andreza Lemos Salvio, Otávio José Brustolini, Alexandra Lehmkuhl Gerber, Ana Paula de Campos Guimarães, Carla Augusta Barreto Marques, Renan Amphilophio Fernandes, Carlos Henrique Ferreira Ramos Filho, and et al. 2022. "Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients" International Journal of Molecular Sciences 23, no. 21: 13588. https://doi.org/10.3390/ijms232113588