Phytochemical and Antioxidant Profile of the Medicinal Plant Melia azedarach Subjected to Water Deficit Conditions
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
3.1. Plant Material and Stress Treatment
3.2. Plant Water Status
3.3. Total Antioxidant Activity, Total Polyphenols, Catechols, and Flavonoids
3.4. Extraction of Metabolites and Chromatography Analysis
3.5. Statistical Analysis
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Intergovernmental Panel on Climate Change (IPCC). Climate Change and Land: An IPCC Special Report on Climate Change, Desertification, Land Degradation, Sustainable Land Management, Food Security, and Greenhouse Gas Fluxes in Terrestrial Ecosystems; IPCC: Geneva, Switzerland, 2019. [Google Scholar]
- Morin, X.; Fahse, L.; Jactel, H.; Scherer-Lorenzen, M.; García-Valdés, R.; Bugmann, H. Long-term response of forest productivity to climate change is mostly driven by change in tree species composition. Sci. Rep. 2018, 8, 5627. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Ma, Y.; Dias, M.C.; Freitas, H. Drought and Salinity Stress Responses and Microbe-Induced Tolerance in Plants. Front. Plant Sci. 2020, 11, 591911. [Google Scholar] [CrossRef] [PubMed]
- Huang, H.; Ullah, F.; Zhou, D.-X.; Yi, M.; Zhao, Y. Mechanisms of ROS Regulation of Plant Development and Stress Responses. Front. Plant Sci. 2019, 10, 800. [Google Scholar] [CrossRef] [PubMed]
- Shah, A.; Smith, D.L. Flavonoids in Agriculture: Chemistry and Roles in, Biotic and Abiotic Stress Responses, and Microbial Associations. Agronomy 2020, 10, 1209. [Google Scholar] [CrossRef]
- Brunetti, C.; di Ferdinando, M.; Fini, A.; Pollastri, S.; Tattini, M. Flavonoids as Antioxidants and Developmental Regulators: Relative Significance in Plants and Humans. Int. J. Mol. Sci. 2013, 14, 3540–3555. [Google Scholar] [CrossRef] [Green Version]
- Dias, M.C.; Pinto, D.C.; Figueiredo, C.; Santos, C.; Silva, A.M. Phenolic and lipophilic metabolite adjustments in Olea europaea (olive) trees during drought stress and recovery. Phytochemistry 2021, 185, 112695. [Google Scholar] [CrossRef] [PubMed]
- Dias, M.C.; Pinto, D.C.; Correia, C.; Moutinho-Pereira, J.; Oliveira, H.; Freitas, H.; Silva, A.M.; Santos, C. UV-B radiation modulates physiology and lipophilic metabolite profile in Olea europaea. J. Plant Physiol. 2018, 222, 39–50. [Google Scholar] [CrossRef]
- Geronço, M.S.; Melo, R.C.; Barros, H.L.M.; Aquino, S.R.; de Oliveira, F.d.C.E.; Islam, M.T.; do Pessoa, C.Ó.; dos Rizzo, M.S.; da Costa, M.P. Advances in the research of Adenanthera pavonina: From traditional use to intellectual property. J. Med. Plants Res. 2020, 14, 24–53. [Google Scholar] [CrossRef] [Green Version]
- Gupta, A.; Rico-Medina, A.; Caño-Delgado, A.I. The physiology of plant responses to drought. Science 2020, 368, 266–269. [Google Scholar] [CrossRef]
- Kapoor, D.; Bhardwaj, S.; Landi, M.; Sharma, A.; Ramakrishnan, M.; Sharma, A. The Impact of Drought in Plant Metabolism: How to Exploit Tolerance Mechanisms to Increase Crop Production. Appl. Sci. 2020, 10, 5692. [Google Scholar] [CrossRef]
- Gupta, A.; Singh, P.P.; Singh, P. Medicinal plants. In Climate Change and Agricultural Ecosystems; Choudhary, K.K., Kumar, A., Singh, A.K., Eds.; Elsevier: Amsterdam, The Netherlands, 2019; pp. 181–209. [Google Scholar] [CrossRef]
- Araújo, M.; Prada, J.; Mariz-Ponte, N.; Santos, C.; Pereira, J.; Pinto, D.; Silva, A.; Dias, M. Antioxidant Adjustments of Olive Trees (Olea europaea) under Field Stress Conditions. Plants 2021, 10, 684. [Google Scholar] [CrossRef] [PubMed]
- Li, Y.; Kong, D.; Fu, Y.; Sussman, M.R.; Wu, H. The effect of developmental and environmental factors on secondary metabolites in medicinal plants. Plant Physiol. Biochem. 2020, 148, 80–89. [Google Scholar] [CrossRef] [PubMed]
- Rogowska, A.; Szakiel, A. The role of sterols in plant response to abiotic stress. Phytochem. Rev. 2020, 19, 1525–1538. [Google Scholar] [CrossRef]
- Omidi, H.; Shams, H.; Seif Sahandi, M.; Radjabian, T. Balangu (Lallemantia sp.) growth and physiology under field drought conditions affecting plant medicinal content. Plant Physiol. Biochem. 2018, 130, 641–646. [Google Scholar] [CrossRef] [PubMed]
- Applequist, J.; Burroughs, C.; Ramirez, A.; Merkel, P.A.; Rothenberg, M.E.; Trapnell, B.; Desnick, R.J.; Sahin, M.; Krischer, J.P. A novel approach to conducting clinical trials in the community setting: Utilizing patient-driven platforms and social media to drive web-based patient recruitment. BMC Med. Res. Methodol. 2020, 20, 58. [Google Scholar] [CrossRef] [Green Version]
- Chauhan, S.K.; Kumar, A.; Id, D.; Sharma, R. Growth dynamics of different half-sib families of Melia azedarach Linn. PLoS ONE 2018, 13, e0207121. [Google Scholar] [CrossRef]
- Dadé, M.; Zeinsteger, P.; Bozzolo, F.; Mestorino, N. Repellent and Lethal Activities of Extracts from Fruits of Chinaberry (Melia azedarach L., Meliaceae) against Triatoma infestans. Front. Vet. Sci. 2018, 5, 158. [Google Scholar] [CrossRef] [Green Version]
- Hammad, E.A.F. Chinaberry, Melia azedarach, L., A Biopesticidal Tree. In Encyclopedia of Entomology; Springer: Dordrecht, The Netherlands, 2004; pp. 499–504. [Google Scholar] [CrossRef]
- Feng, L.; Tian, X.; El-Kassaby, Y.A.; Qiu, J.; Feng, Z.; Sun, J.; Wang, G.; Wang, T. Predicting suitable habitats of Melia azedarach L. in China using data mining. Sci. Rep. 2022, 12, 12617. [Google Scholar] [CrossRef]
- Dias, M.C.; Azevedo, C.; Costa, M.; Pinto, G.; Santos, C. Melia azedarach plants show tolerance properties to water shortage treatment: An ecophysiological study. Plant Physiol. Biochem. 2014, 75, 123–127. [Google Scholar] [CrossRef]
- Nair, S.; Marimuthu, S.; Padmaja, B. Phytochemical screening studies on Melia orientalis by GC-MS analysis. Pharmacogn. Res. 2013, 5, 216–218. [Google Scholar] [CrossRef] [Green Version]
- Sharma, D.; Paul, Y. Preliminary and pharmacological profile of Melia azedarach L.: An Overview. J. Appl. Pharm. Sci. 2013, 3, 133–138. [Google Scholar] [CrossRef]
- Della Bona, A.; Nedel, F. Evaluation of Melia azedarach Extracts against Streptococcus mutans. J. Med. Food 2015, 18, 259–263. [Google Scholar] [CrossRef] [PubMed]
- Khoshraftar, Z.; Safekordi, A.A.; Shamel, A.; Zaefizadeh, M. Evaluation of insecticidal activity of nanoformulation of Melia azedarach (leaf) extract as a safe environmental insecticide. Int. J. Environ. Sci. Technol. 2020, 17, 1159–1170. [Google Scholar] [CrossRef]
- Dias, M.C.; Oliveira, H.; Costa, A.; Santos, C. Improving elms performance under drought stress: The pretreatment with abscisic acid. Environ. Exp. Bot. 2014, 100, 64–73. [Google Scholar] [CrossRef]
- Fahad, S.; Bajwa, A.A.; Nazir, U.; Anjum, S.A.; Farooq, A.; Zohaib, A.; Sadia, S.; Nasim, W.; Adkins, S.; Saud, S.; et al. Crop Production under Drought and Heat Stress: Plant Responses and Management Options. Front. Plant Sci. 2017, 8, 1147. [Google Scholar] [CrossRef] [Green Version]
- Dias, M.C.; Correia, S.; Serôdio, J.; Silva, A.M.S.; Freitas, H.; Santos, C. Chlorophyll fluorescence and oxidative stress endpoints to discriminate olive cultivars tolerance to drought and heat episodes. Sci. Hortic. 2018, 231, 31–35. [Google Scholar] [CrossRef]
- Ma, D.Y.; Sun, D.X.; Wang, C.Y.; Li, Y.G.; Guo, T.C. Expression of flavonoid biosynthesis genes and accumulation of flavonoid in wheat leaves in response to drought stress. Plant Physiol. Biochem. 2014, 80, 60–66. [Google Scholar] [CrossRef]
- Sharma, A.; Shahzad, B.; Rehman, A.; Bhardwaj, R.; Landi, M.; Zheng, B. Response of Phenylpropanoid Pathway and the Role of Polyphenols in Plants under Abiotic Stress. Molecules 2019, 24, 2452. [Google Scholar] [CrossRef]
- Ervina, M.; Sukardiman. A review: Melia azedarach L. as a potent anticancer drug. Pharmacogn. Rev. 2018, 12, 94. [Google Scholar] [CrossRef]
- Orhan, I.E.; Guner, E.; Ozturk, N.; Senol, F.; Erdem, S.A.; Kartal, M.; Sener, B. Enzyme inhibitory and antioxidant activity of Melia azedarach L. naturalized in Anatolia and its phenolic acid and fatty acid composition. Ind. Crops Prod. 2012, 37, 213–218. [Google Scholar] [CrossRef]
- Lv, Y.; Tahir, I.I.; Olsson, M.E. Factors affecting the content of the ursolic and oleanolic acid in apple peel: Influence of cultivars, sun exposure, storage conditions, bruising and Penicillium expansum infection. J. Sci. Food Agric. 2016, 96, 2161–2169. [Google Scholar] [CrossRef] [PubMed]
- Sokoła-Wysoczańska, E.; Wysoczański, T.; Wagner, J.; Czyż, K.; Bodkowski, R.; Lochyński, S.; Patkowska-Sokoła, B. Polyunsaturated Fatty Acids and Their Potential Therapeutic Role in Cardiovascular System Disorders—A Review. Nutrients 2018, 10, 1561. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chen, J.; Song, Y.; Zhang, H.; Zhang, D. Genome-Wide Analysis of Gene Expression in Response to Drought Stress in Populus simonii. Plant Mol. Biol. Rep. 2013, 31, 946–962. [Google Scholar] [CrossRef]
- Carocho, M.; Barros, L.; Antonio, A.L.; Barreira, J.C.; Bento, A.; Kaluska, I.; Ferreira, I.C. Analysis of organic acids in electron beam irradiated chestnuts (Castanea sativa Mill.): Effects of radiation dose and storage time. Food Chem. Toxicol. 2013, 55, 348–352. [Google Scholar] [CrossRef] [PubMed]
- Ferro, A.; Carbone, E.; Zhang, J.; Marzouk, E.; Villegas, M.; Siegel, A.; Nguyen, D.; Possidente, T.; Hartman, J.; Polley, K.; et al. Short-term succinic acid treatment mitigates cerebellar mitochondrial OXPHOS dysfunction, neurodegeneration and ataxia in a Purkinje-specific spinocerebellar ataxia type 1 (SCA1) mouse model. PLoS ONE 2017, 12, e0188425. [Google Scholar] [CrossRef] [Green Version]
- Kim, S.-C.; Faure, L.; Chapman, K.D. Analysis of Fatty Acid Amide Hydrolase Activity in Plants. In Plant Lipid Signaling Protocols; Munnik, T., Heilmann, I., Eds.; Humana Press: Totowa, NJ, USA, 2013; Volume 1009, pp. 115–127. [Google Scholar] [CrossRef]
- Ge, L.; Zhu, M.-M.; Yang, J.-Y.; Wang, F.; Zhang, R.; Zhang, J.-H.; Shen, J.; Tian, H.-F.; Wu, C.-F. Differential proteomic analysis of the anti-depressive effects of oleamide in a rat chronic mild stress model of depression. Pharmacol. Biochem. Behav. 2015, 131, 77–86. [Google Scholar] [CrossRef] [PubMed]
- Wu, T.-T.; Charles, A.L.; Huang, T.-C. Determination of the contents of the main biochemical compounds of Adlay (Coxi lachrymal-jobi). Food Chem. 2007, 104, 1509–1515. [Google Scholar] [CrossRef]
- Lu, Y.; Zhou, Y.; Nakai, S.; Hosomi, M.; Zhang, H.; Kronzucker, H.; Shi, W. Stimulation of nitrogen removal in the rhizosphere of aquatic duckweed by root exudate components. Planta 2013, 239, 591–603. [Google Scholar] [CrossRef] [Green Version]
- Sherein, I.A.E.M.; Mohamed, A.A.; Ahmed, M.G.; Manal, F.A.A. In vitro antibacterial activities of dietary medicinal ethanolic extracts against pathogenic reference strains of animal origin. Afr. J. Microbiol. Res. 2013, 7, 5261–5270. [Google Scholar] [CrossRef]
- Aboobucker, S.I.; Suza, W.P. Why Do Plants Convert Sitosterol to Stigmasterol? Front. Plant Sci. 2019, 10, 354. [Google Scholar] [CrossRef] [Green Version]
- Piccini, C.; Cai, G.; Dias, M.C.; Araújo, M.; Parri, S.; Romi, M.; Faleri, C.; Cantini, C. Olive Varieties under UV-B Stress Show Distinct Responses in Terms of Antioxidant Machinery and Isoform/Activity of RubisCO. Int. J. Mol. Sci. 2021, 22, 11214. [Google Scholar] [CrossRef] [PubMed]
- AbuMweis, S.S.; Vanstone, C.A.; Lichtenstein, A.H.; Jones, P.J.H. Plant sterol consumption frequency affects plasma lipid levels and cholesterol kinetics in humans. Eur. J. Clin. Nutr. 2008, 63, 747–755. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Rosa, M.; Prado, C.; Podazza, G.; Interdonato, R.; González, J.A.; Hilal, M.; Prado, F.E. Soluble sugars-metabolism, sensing and abiotic stress a complex network in the life of plants. Plant Signal. Behav. 2009, 4, 388–393. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- You, J.; Chan, Z. ROS Regulation During Abiotic Stress Responses in Crop Plants. Front. Plant Sci. 2015, 6, 1092. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Goswami, L.; Sengupta, S.; Mukherjee, S.; Ray, S.; Mukherjee, R.; Majumder, A.L. Targeted expression of L-myo-inositol 1-phosphate synthase from Porteresia coarctata (Roxb.) Tateoka confers multiple stress tolerance in transgenic crop plants. J. Plant Biochem. Biotechnol. 2014, 23, 316–330. [Google Scholar] [CrossRef]
- DiNicola, S.; Minini, M.; Unfer, V.; Verna, R.; Cucina, A.; Bizzarri, M. Nutritional and Acquired Deficiencies in Inositol Bioavailability. Correlations with Metabolic Disorders. Int. J. Mol. Sci. 2017, 18, 2187. [Google Scholar] [CrossRef] [Green Version]
- Re, R.; Pellegrini, N.; Proteggente, A.; Pannala, A.; Yang, M.; Rice-Evans, C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 1999, 26, 1231–1237. [Google Scholar] [CrossRef]
- Giertych, M.J.; Karolewski, P.; de Temmerman, L.O. Foliage Age and Pollution Alter Content of Phenolic Compounds and Chemical Elements in Pinus nigra Needles. Water Air Soil Pollut. 1999, 110, 363–377. [Google Scholar] [CrossRef]
Rt (min) | Compound | Treatments (mg g−1 DW) | p-Value | Fold Change | |
---|---|---|---|---|---|
WW | WD | ||||
Long-chain alkanes | |||||
56.33 | Tetracontane | 2.10 ± 0.018 | 2.35 ± 0.039 * | <0.001 | |
61.12 | Hexatriacontane | 2.74 ± 0.105 | 3.48 ± 0.315 * | 0.019 | |
66.16 | Dopentacontane | 3.28 ± 0.513 | 2.77 ± 0.066 | 0.165 | |
Sterols and polyalcohol | |||||
64.43 | β-Sitosterol | 1.32 ± 0.097 | 1.94 ± 0.021 * | <0.001 | |
64.90 | Stigmasterol | 1.27 ± 0.110 | 1.24 ± 0.121 | 0.727 | |
60.44 | Campesterol | 1.41 ± 0.086 | 1.28 ± 0.036 | 0.072 | |
39.16 | Myo-inositol | 0.10 ± 0.02 | 0.58 ± 0.041 * | <0.001 | |
Amides and organic acids | |||||
45.57 | Oleamide | 3.42 ± 0.499 | 3.30 ± 0.345 | 0.758 | |
42.87 | Stearic acid | 2.81 ± 0.017 | 3.02 ± 0.105 * | 0.027 | |
42.26 | α-Linolenic acid | 2.91 ± 0.131 | 3.59 ± 0.128 * | 0.003 | |
41.52 | Linoleic acid | 3.38 ± 0.236 | 2.73 ± 0.034 * | 0.040 | |
38.46 | Palmitic acid | 3.45 ± 0.190 | 3.99 ± 0.104 * | 0.013 | |
22.25 | Succinic acid | 2.79 ± 0.005 | 3.72 ± 0.167 * | <0.001 | |
Carbohydrates | |||||
50.52 | Sucrose | 0.348 ± 0.055 | 4.54 ± 0.190 * | <0.001 | |
41.85 | Melibiose | 0.324 ± 0.006 | 0.555 ± 0.153 | 0.059 | |
36.90 | d-Glucose | 0.390 ± 0.072 | 1.43 ± 0.034 * | <0.001 | |
34.50 | β-d-Glucopyranose | 0.331 ± 0.029 | 1.16 ± 0.008 * | <0.001 | |
33.46 | d-(+)-Talofuranose | 0.386 ± 0.037 | 0.378 ± 0.012 | 0.729 | |
32.57 | d-Psicofuranose | 0.357 ± 0.044 | 0.680 ± 0.062 * | 0.002 | |
32.35 | d-(+)-Fructofuranose | 0.361 ± 0.046 | 0.690 ± 0.002 * | <0.001 |
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Dias, M.C.; Pinto, D.C.G.A.; Costa, M.; Araújo, M.; Santos, C.; Silva, A.M.S. Phytochemical and Antioxidant Profile of the Medicinal Plant Melia azedarach Subjected to Water Deficit Conditions. Int. J. Mol. Sci. 2022, 23, 13611. https://doi.org/10.3390/ijms232113611
Dias MC, Pinto DCGA, Costa M, Araújo M, Santos C, Silva AMS. Phytochemical and Antioxidant Profile of the Medicinal Plant Melia azedarach Subjected to Water Deficit Conditions. International Journal of Molecular Sciences. 2022; 23(21):13611. https://doi.org/10.3390/ijms232113611
Chicago/Turabian StyleDias, Maria Celeste, Diana C. G. A. Pinto, Maria Costa, Márcia Araújo, Conceição Santos, and Artur M. S. Silva. 2022. "Phytochemical and Antioxidant Profile of the Medicinal Plant Melia azedarach Subjected to Water Deficit Conditions" International Journal of Molecular Sciences 23, no. 21: 13611. https://doi.org/10.3390/ijms232113611