Interaction of Influenza A Nucleoprotein with Host hnRNP-C Is Implicated in Viral Replication
Abstract
:1. Introduction
2. Results
2.1. Host Heterogeneous Nuclear Ribonucleoprotein C (hnRNP-C) Was Identified as an Interacting Partner of NP
2.2. NP and hnRNP-C Directly Bind to One Another
2.3. Characterization of NP–hnRNP-C Interaction
2.4. hnRNP-C Is Implicated in Influenza Virus Replication
2.5. Perturbation of NP–hnRNP-C Interaction Reduces Ribonucleoprotein (RNP) Activity
3. Discussion
4. Materials and Methods
4.1. Plasmids
4.2. Cells
4.3. Antibodies
4.4. Expression of NP, Pull-Down, and Mass Spectrometric Analysis
4.5. Virus Infection
4.6. Co-Immunoprecipitation
4.7. In Vitro NHS Pull-Down
4.8. Reverse Transcription qPCR (RT-qPCR)
4.9. Knockdown of hnRNP-C
4.10. Minigenome Luciferase Reporter Assay
4.11. Fluorescence Staining
4.12. Western Blot
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Tang, Y.-S.; So, W.-K.; Ng, K.-L.A.; Mok, K.-P.C.; Shaw, P.-C. Interaction of Influenza A Nucleoprotein with Host hnRNP-C Is Implicated in Viral Replication. Int. J. Mol. Sci. 2022, 23, 13613. https://doi.org/10.3390/ijms232113613
Tang Y-S, So W-K, Ng K-LA, Mok K-PC, Shaw P-C. Interaction of Influenza A Nucleoprotein with Host hnRNP-C Is Implicated in Viral Replication. International Journal of Molecular Sciences. 2022; 23(21):13613. https://doi.org/10.3390/ijms232113613
Chicago/Turabian StyleTang, Yun-Sang, Wai-Kin So, Ka-Leung Andy Ng, Ka-Pun Chris Mok, and Pang-Chui Shaw. 2022. "Interaction of Influenza A Nucleoprotein with Host hnRNP-C Is Implicated in Viral Replication" International Journal of Molecular Sciences 23, no. 21: 13613. https://doi.org/10.3390/ijms232113613