The Amino Acid Permease MoGap1 Regulates TOR Activity and Autophagy in Magnaporthe oryzae
Abstract
:1. Introduction
2. Results
2.1. Identification and Knockout of MoGAP1 in M. oryzae
2.2. MoGap1 Regulates the Formation of the Conidia
2.3. Increased Sensitivity to Rapamycin in MoGAP1-Deleted Mutants
2.4. MoGap1 Negatively Regulates Autophagy
2.5. MoGap1 Responds to Cell Wall Stress and Carbon Source Stress
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Test Strains and Plants
5.2. Gene Knockout and Complementation
5.3. Pathogenicity Detection
5.4. Cell Staining and Stress Test
5.5. Western Blot Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Huang, C.; Li, L.; Wang, L.; Bao, J.; Zhang, X.; Yan, J.; Wu, J.; Cao, N.; Wang, J.; Zhao, L.; et al. The Amino Acid Permease MoGap1 Regulates TOR Activity and Autophagy in Magnaporthe oryzae. Int. J. Mol. Sci. 2022, 23, 13663. https://doi.org/10.3390/ijms232113663
Huang C, Li L, Wang L, Bao J, Zhang X, Yan J, Wu J, Cao N, Wang J, Zhao L, et al. The Amino Acid Permease MoGap1 Regulates TOR Activity and Autophagy in Magnaporthe oryzae. International Journal of Molecular Sciences. 2022; 23(21):13663. https://doi.org/10.3390/ijms232113663
Chicago/Turabian StyleHuang, Changli, Lin Li, Lei Wang, Jiandong Bao, Xiaozhi Zhang, Jiongyi Yan, Jiaqi Wu, Na Cao, Jiaoyu Wang, Lili Zhao, and et al. 2022. "The Amino Acid Permease MoGap1 Regulates TOR Activity and Autophagy in Magnaporthe oryzae" International Journal of Molecular Sciences 23, no. 21: 13663. https://doi.org/10.3390/ijms232113663