Transcriptomic and Metabolomic Analyses Reveal That Fullerol Improves Drought Tolerance in Brassica napus L
Abstract
:1. Introduction
2. Results
2.1. Aboveground Biomass and Leaf Relative Water Content
2.2. Transcriptomic Analysis
2.2.1. Analysis of Differentially Expressed Genes (DEGs)
2.2.2. Functional Analysis by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG)
2.2.3. Quantitative Real-Time (qRT)-PCR
2.3. Metabolic Analysis
2.4. Data Integration/Comprehensive Networks of Transcripts and Metabolites
2.4.1. Carbohydrate Metabolism
2.4.2. Amino Acid Metabolism
2.4.3. Secondary Metabolite Metabolism
3. Discussion
3.1. Carbohydrate Metabolism
3.2. Amino Acid Metabolism
3.3. Secondary Metabolite Metabolism
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. RNA-Seq Analysis
4.2.1. RNA Extraction and Quantification
4.2.2. Data Processing
4.2.3. qRT-PCR Analysis
4.3. Metabolomic Analysis
4.3.1. Metabolite Extraction and LC-MS Conditions
4.3.2. Multivariate Data Processing
4.4. Statistical Analyses of Other Data
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Xiong, J.-L.; Ma, N. Transcriptomic and Metabolomic Analyses Reveal That Fullerol Improves Drought Tolerance in Brassica napus L. Int. J. Mol. Sci. 2022, 23, 15304. https://doi.org/10.3390/ijms232315304
Xiong J-L, Ma N. Transcriptomic and Metabolomic Analyses Reveal That Fullerol Improves Drought Tolerance in Brassica napus L. International Journal of Molecular Sciences. 2022; 23(23):15304. https://doi.org/10.3390/ijms232315304
Chicago/Turabian StyleXiong, Jun-Lan, and Ni Ma. 2022. "Transcriptomic and Metabolomic Analyses Reveal That Fullerol Improves Drought Tolerance in Brassica napus L" International Journal of Molecular Sciences 23, no. 23: 15304. https://doi.org/10.3390/ijms232315304