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Article

Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R

by
Jixiu Wang
1,2,
Qianhui Sun
1,2,
Jian Zhang
1,2,
Huanling Wang
1,2 and
Hong Liu
1,2,*
1
Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair/Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China
2
Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan 430070, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2022, 23(4), 2019; https://doi.org/10.3390/ijms23042019
Submission received: 23 January 2022 / Revised: 9 February 2022 / Accepted: 10 February 2022 / Published: 11 February 2022
(This article belongs to the Section Molecular Immunology)
Browse Figures
Versions Notes

Abstract

:
Interleukin-6 (IL-6) is a multipotent cytokine. IL-6 plays a dual role in inflammation through both classical signaling (IL-6 binds membrane IL-6 receptor/IL-6R) and trans-signaling (IL-6 binds soluble IL-6R). However, the regulation of IL-6 activity, especially the regulation of signaling pathways and downstream genes mediated by IL-6 trans-signaling, remains largely unclear in teleost. Grass carp (Ctenopharyngodon idellus) hepatic (L8824) cells, kidney (CIK) cells, and primary hepatocytes were used as test models in this study. First, the biological activity of recombinant blunt snout bream (Megalobrama amblycephala) IL-6 (rmaIL-6) and sIL-6R (rmasIL-6R) was verified by quantitative PCR (qPCR) and western blot. The western blot results showed that rmaIL-6 significantly upregulated signal transducer and activator of transcription 3 (STAT3) phosphorylation in L8824 cells and primary hepatocytes, while rmaIL-6 in combination with rmasIL-6R (rmaIL-6+rmasIL-6R) significantly upregulated STAT3 phosphorylation in all types of cells. Furthermore, maIL-6 and maIL-6+rmasIL-6R could only induce extracellular-signal-regulated kinase 1/2 (ERK1/2) phosphorylation in L8824 cells and CIK cells, respectively. Therefore, IL-6 mainly acts by activating the janus kinase (JAK)/STAT3 pathway rather than the mitogen-activated protein kinase (MEK)/ERK pathway. Finally, the activation of the JAK2/STAT3 pathway was shown to be essential for the generation of socs3a and socs3b induced by IL-6 trans-signaling after treatment by JAK2/STAT3 pathway inhibitors (c188-9 and TG101348). These findings provide functional insights into IL-6 classical signaling and trans-signaling regulatory mechanisms in teleost, enriching our knowledge of fish immunology.

1. Introduction

Interleukin-6 (IL-6) was first identified in humans as B cell stimulatory factor 2 (BSF-2), which induces B cells to produce immunoglobulin (Ig) [1]. IL-6 is produced by several cell types including monocytes/macrophages, endothelial cells, adipocytes, and hematopoietic cells and acts on a wide range of cell types such as T/B cells, macrophages, hepatic cells, vascular endothelial cells, and hematopoietic stem cells [1,2,3,4,5,6,7]. As a typical cytokine, IL-6 has pleiotropic effects in inflammation, immune response, and hematopoiesis [8,9].
When IL-6 sends signals into cells, it binds to a heterodimeric receptor complex consisting of a high-affinity chain interleukin-6 receptor (IL-6R) and glycoprotein 130 (gp130), and then triggers two major signaling pathways: the extracellular-signal-regulated kinase (ERK)/mitogen-activated protein kinase (MEK) pathway and the Janus kinases (JAK)/signal transducer and activator of transcription 3 (STAT3) pathway [10]. The inflammatory cytokine IL-6 upregulates antimicrobial peptides (hamp) expression by activating the IL-6R/JAK2/STAT3 pathway [11]. STAT3 regulates its own endogenous inhibitor, SOCS3, forming a negative feedback loop [12]. In rats, IL-6 induces the activation of the STAT3/SOCS3 pathway in the liver, but not other downstream pathways such as STAT1, ERK1/2, and PI3K [13]. In human cells, the JAK2/STAT3 signaling pathway has been shown to promote IL-6 expression [14,15].
The proinflammatory and anti-inflammatory effects of IL-6 appear to originate from its capacity to activate multiple signaling pathways in a cell type-specific manner [16,17]. For classical signaling, IL-6 binds to membrane-bound IL-6R (mIL-6R) and activates intracellular signaling cascades via gp130. IL-6 classical signaling is primarily limited to hepatocytes and immune cells (macrophages and certain other leukocyte populations), which express IL-6R on their surface [18,19]. Thus, the number of cells targeted by IL-6 classical signaling is restricted. The body also produces a soluble IL-6 receptor (sIL-6R) that is released into the circulation after proteolytic cleavage of the mIL-6R protein or following translation from alternatively spliced mRNA [20,21]. Signaling by IL-6 in combination with sIL-6R is called trans-signaling. Due to the uniform distribution of gp130, this excitatory IL-6 and sIL-6R complex can, in principle, activate all cells [20]. The two different signaling events have divergent functions. Classical signaling is associated with regenerative and anti-inflammatory functions, while trans-signaling is linked to pro-inflammatory functions [22,23,24,25].
Inhibitors have been widely used in the study of signaling pathways and in clinical medicine. c188-9, a small-molecule inhibitor of STAT3, targets the phosphotyrosyl peptide binding site within the STAT3 SH2 domain and does not inhibit upstream JAK or Src kinases [26]. c188-9 has been used to inhibit the phosphorylation of STAT3 and the proliferation of cancer cells [27,28]. On the other hand, Fedratinib (TG101348) is a selective JAK2 inhibitor that is indicated for the treatment of adults with intermediate-2 or high-risk primary or secondary myelofibrosis [29].
The il-6 gene has been identified in several teleost, such as zebrafish (Danio rerio, Hamilton 1822) [30], fugu (Takifugu rubripes, Temminck & Schlegel 1850) [31], Paralichthys olivaceus (Temminck & Schlegel, 1846) [32], Sparus aurata (Linnaeus, 1758) [33], Larimichthys crocea (Richardson, 1846) [34], rainbow trout (Oncorhynchus mykiss, Walbaum 1792) [35], and blunt snout bream (Megalobrama amblycephala, Yi Bolu 1955) [36]. In teleost, IL-6 has been proved to play vital roles in the immune response, inducing the expression of hamp and regulating cytokines genes (il-1β and il-6) and socs1–3 [33,37,38]. Additionally, in fugu, IL-6R was cloned for the first time, confirming that IL-6 and sIL-6R activated STAT3 phosphorylation [39]. In grass carp, sIL-6R can participate in the immunoregulatory function of IL-6, thereby further upregulating the expression of socs3 [40]. In Nile tilapia (Oreochromis niloticus, Linnaeus 1758), sIL-6R could combine with IL-6 to promote the upregulation of il-1β and il-6 and has an agonistic effect in IL-6-mediated inflammation [41]. Due to its pronounced immunoregulatory effects, IL-6 and sIL-6R might be essential for the initiation of immune defenses in fish. However, little is known about IL-6 classical signaling and trans-signaling transduction mechanisms in fish.
The blunt snout bream il-6 gene could be strongly induced in Aeromonas hydrophila (Kluyver & Van Niel, 1936) in many tissues, indicating that il-6 plays an active role in the defense against pathogens [36,42]. IL-6 amino acid sequence in blunt snout bream (maIL-6) shares 91% identity with that of grass carp (Ctenopharyngodon idellus, Cuvier et Valenciennes 1844) (ciIL-6), but only 23–30% identity with those of mammals, birds, and amphibians [36]. Therefore, to explore the regulation mechanisms of IL-6 classical signaling and trans-signaling in fish, we first verified whether L8824 cells (grass carp hepatocyte cells with IL-6R) can receive signals from recombinant IL-6 and whether recombinant maIL-6 (rmaIL-6) has biological activity. Whereafter, the activation of the JAK/STAT3 and MEK/ERK pathways in L8824 cells, CIK cells (grass carp kidney cells without IL-6R) [40], and primary hepatocytes of grass carp was investigated. Additionally, whether rmaIL-6+rmasIL-6R regulates the expression of downstream genes (il-6, hamp, socs3a, and socs3b) through the JAK2/STAT3 pathway was verified.

2. Results

2.1. Sequence Analysis of maIL-6 and masIL-6R

As shown in Supplementary Figure S1A, the cDNA sequence of mail-6 is 1045 bp and contains an open reading frame (ORF) of 699 bp, encoding a 232 aa protein with a signal peptide of 24 aa. The full-length cDNA of mail-6r is 3739 bp, with an ORF of 1797 bp, encoding 598 aa. The signal peptide region of maIL-6R is 1–21 aa-long, the extracellular region comprises 1–491 aa, the transmembrane region 492–514 aa, and the intracellular region 515–598 aa (Supplementary Figure S1B).
To analyze homology of IL-6 between grass carp (C. idellus) and blunt snout bream (M. amblycephala), multiple sequence alignment was performed. The analysis showed that the similarity in amino acid sequence of IL-6 and sIL-6R between blunt snout bream and grass carp was as high as 90.91% and 86.89%, respectively (Figure 1A,B). However, the IL-6 proteins from grass carp and blunt snout bream showed some differences in secondary structure and solvent accessibility (Figure 1C).

2.2. Effects of Recombinant IL-6 on the Expression of Downstream Genes in L8824 Cells

As shown in Figure 2, recombinant grass carp IL-6 (rciIL-6) and recombinant blunt snout bream IL-6 (rmaIL-6) proteins were successfully produced in the inclusion bodies of Escherichia coli. The purified rciIL-6 (Figure 2A) and rmaIL-6 (Figure 2B) were visualized by similar single bands around 40 kDa (theoretical MW: 24.27 kDa for rciIL-6 or 24.15 kDa for rmaIL-6 and 18.3 kDa of pET-32a plasmid tag protein) on an SDS-PAGE gel.
To evaluate the biological activity of recombinant IL-6, the expression of downstream genes including hamp, il-1β, il-6, socs3a, and socs3b in L8824 cells was analyzed. As shown in Figure 2C, after 4 h of stimulation, the expression of hamp il-1β and il-6 was induced by 0.5 and 1.0 μg/mL of rciIL-6 protein, while socs3b expression was inhibited by 0.5 μg/mL of rciIL-6. Similarly, as shown in Figure 2D, after 2 h of stimulation, the expression of hamp and socs3b could not be significantly modulated by rmaIL-6, but il-6 (at all three doses), il-1β (at 1.5 μg/mL), and socs3a (at all three doses) could be induced by rmaIL-6.
As shown in Figure 2E, when L8824 cells were treated with rciIL-6 for different times, the mRNA level of hamp was significantly upregulated at 4 h and then gradually decreased to the control level, whereas, after rmaIL-6 treatment, the expression of hamp peaked at 24 h. In addition, rciIL-6 increased the il-6 mRNA level only at 4 h, while rmaIL-6 could significantly upregulate the expression of il-6 mRNA at 2, 4, and 24 h of stimulation. rciIL-6 significantly downregulated socs3a at 24 h, whereas rmaIL-6 significantly upregulated socs3a expression at 2 h and 36 h. In addition, rciIL-6 significantly increased socs3b expression only at 8 h, while rmaIL-6 significantly induced socs3b expression at both 4 h and 12 h.

2.3. Activation of Signaling Pathways by rmaIL-6 with or without RmasIL-6R

As shown in Figure 3A, rmasIL-6R was successfully obtained. The SDS-PAGE results revealed that the molecular mass of rmasIL-6R is about 60 kDa (the predicted MW of rmasIL-6R is 52.78 kDa, and that of His-tag is ~4.8 kDa), which is consistent with the theoretical molecular mass.
The gp130 CDS fragment could be detected in L8824 cells, CIK cells, and primary hepatocytes cDNA template, and the il-6r CDS fragment could be detected in L8824 cells and primary hepatocytes cDNA template, but not in CIK cells (Supplementary Figure S2). As shown by western blot, in L8824 cells, STAT3 phosphorylation was induced by rmaIL-6 at μg/mL and 1.5 μg/mL, but rmaIL-6 at 1.5 μg/mL concentration was more effective. (Supplementary Figure S3). In CIK cells, STAT3 phosphorylation was induced by rmaIL-6 combined with lower concentrations of rmasIL-6R (i.e., 0.5 μg/mL) (Figure 3B).
In order to investigate the involvement of rmaIL-6 in the JAK/STAT3 signaling pathway, L8824 cells were treated with rmaIL-6 alone (classical signaling) or together with rmasIL-6R (trans-signaling) for different times. The phosphorylation of ERK1/2 and STAT3 was then analyzed using western blot. The results showed that rmaIL-6 alone or rmaIL-6+rmasIL-6R could induce STAT3 phosphorylation. Although rmaIL-6+rmasIL-6R elicited stronger STAT3 phosphorylation than rmaIL-6 alone, both treatments presented similar kinetics, with phosphorylation peaking after 10–30 min of stimulation (Figure 3C). In addition, rmaIL-6 alone could induce ERK1/2 phosphorylation, peaking at 60 min after treatment. However, ERK1/2 phosphorylation did not change after treatment with rmaIL-6+rmasIL-6R (Figure 3D).
On the other hand, when CIK cells was stimulated with rmaIL-6 and rmasIL-6R alone or jointly, it was found that only rmaIL-6+rmasIL-6R could induce STAT3 phosphorylation (Supplementary Figure S3). Therefore, the phosphorylation of ERK1/2 and STAT3 was detected at different time points after CIK cells were stimulated only by rmaIL-6+rmasIL-6R (Figure 3E). As shown in Figure 3F, CIK cells responded to rmaIL-6 stimulation similarly to L8824 cells, where STAT3 phosphorylation increased significantly at 10 min, peaked at 30 min, and then declined slowly. We then investigated whether IL-6 induced activation of MEK/ERK signaling pathways by measuring the level of the ERK1/2 phosphorylation. Treatment with rmaIL-6+rmasIL-6R caused a strong phosphorylation of ERK1/2 in CIK cells at 60 and 120 min (Figure 3F).
In primary hepatocytes of grass carp, STAT3 phosphorylation was induced by rmaIL-6 or rmaIL-6+rmasIL-6R (Figure 4A,B). The stimulation of primary hepatocytes with rmaIL-6 or rmaIL-6+rmasIL-6R presented a similar kinetics, with a peak of STAT3 phosphorylation at 10 min (Figure 4C). However, neither rmaIL-6 nor rmaIL-6+rmasIL-6R could significantly affect ERK1/2 phosphorylation (Figure 4D).
Moreover, in L8824 cells, STAT3 phosphorylation could be induced by rmaIL-6 alone or in combination with rmasIL-6R, but the effect of the combined stimulation was stronger (Figure 5A). In contrast, CIK cells responded differently to rmaIL-6 stimulation than L8824 cells. In CIK cells, STAT3 phosphorylation could not be induced by rmaIL-6 or rmasIL-6R alone, but only by their combination (Figure 5B). Similar to L8824 cells, STAT3 phosphorylation was induced in primary hepatocytes by rmaIL-6 alone or in combination with rmasIL-6R (Figure 5C)

2.4. RmaIL-6 Trans-Signaling Regulates Socs3a and Socs3b Expression via the JAK2/STAT3 Pathway in L8824 Cells and CIK Cells

To further elucidate the regulation of downstream genes by rmaIL-6 trans-signaling, pharmacological inhibitors were used to interfere with the JAK2/STAT3 pathways, and the expression of il-6, hamp, socs3a, and socs3b was assessed after treatment by rmaIL-6+rmasIL-6R. First, the effects of inhibitors on the phosphorylation of STAT3 or ERK1/2 were examined. As expected, pretreatment of L8824 cells with the STAT3 inhibitor c188-9 inhibited STAT3 phosphorylation, and the JAK2 inhibitor TG101348 strongly inhibited the phosphorylation of STAT3 and ERK1/2 in a dose-dependent manner (Figure 6A,B). Both low and high concentrations of c188-9 did not significantly inhibit STAT3 phosphorylation in CIK cells, while TG101348 could inhibit STAT3 phosphorylation in a dose-dependent manner in CIK cells (Figure 6C,D).
Next, L8824 cells were treated with rmaIL-6+rmasIL-6R after pretreatment with c188-9 or TG101348 for 10 h. As previously shown, in L8824 cells, rmaIL-6+rmasIL-6R could promote STAT3 phosphorylation, while c188-9 reduced STAT3 phosphorylation caused by rmaIL-6+rmasIL-6R (Figure 7A, left). In line with that, qPCR analysis showed that socs3a mRNA induced by rmaIL-6+rmasIL-6R was not detected after c188-9 treatment, while the expression of socs3b was further enhanced by c188-9. Besides, rmaIL-6+rmasIL-6R did not induce the expression of il-6 and hamp, although c188-9 did induce the upregulation of hamp mRNA (Figure 7A, right). Similarly, treating L8824 cells with TG101348 prior to stimulation with rmaIL-6+rmasIL-6R resulted in the suppression of STAT3 phosphorylation (Figure 7B, left). Consistent with this, the induction of socs3a and socs3b by rmaIL-6+rmasIL-6R was inhibited by TG101348 pretreatment (Figure 7B, right).
In CIK cells, pretreatment with the JAK2 inhibitor TG101348 blocked STAT3 phosphorylation induced by rmaIL-6+rmasIL-6R (Figure 7C, left). Inevitably, TG101348 pretreatment could inhibit the expression of socs3a and socs3b induced by rmaIL-6+rmasIL-6R. Surprisingly, hamp expression was strongly induced by TG101348 (Figure 7C, right).

3. Discussion

Cytokines play an important role in the immune system. During IL-6 stimulation, STAT3 phosphorylation increased, while persistent activation of STAT3 contributed to IL-6 production in human basal cells [43]. In this study, rciIL-6 and rmaIL-6 could induce the expression of il-6 in L8824 cells, similar to what observed in rainbow trout [37]. Therefore, IL-6 can increase il-6 expression in an autocrine or paracrine fashion and may amplify and exacerbate the inflammatory response. In our work, both rciIL-6 and rmaIL-6 significantly upregulated the expression of il-1β. Previous studies in teleost showed that recombinant IL-6 protein could not affect the expression of il-1β in L. crocea after 24 h of stimulation [34] and even significantly reduced the expression of il-1β and socs3 in rainbow trout at 24 h [37]. In stark contrast, IL-6 rapidly and dramatically induced il-1β expression in Acipenser baeri (Brandt, 1869) spleen 6 h after treatment [44]. These differences may be due to the different durations of IL-6 stimulation.
IL-6 has been shown to be a necessary and sufficient cytokine to induce hamp expression in mice, human hepatocytes, and cortical neurons [45,46]. Recombinant IL-6 induced the expression of hamp in rainbow trout macrophages [37]. Our results showed that rciIL-6 could rapidly induce the upregulation of hamp in L8824 cells. In addition, rmaIL-6 had no significant effect on hamp expression in a short time but could significantly upregulate hamp at 24 h in L8824 cells. In fish, socs3 is associated with immune regulation as its expression is modulated by inflammatory stimulants, cytokines, and infection [47]. In our work, both rciIL-6 and rmaIL-6 significantly upregulated the expression of socs3b, but rciIL-6 inhibited the expression of socs3a at a certain time point. Therefore, IL-6 in teleost might play both pro-inflammatory and anti-inflammatory roles, but the mechanism is slightly diverse in different species. This difference may be due to structural differences or to a different refolding efficiency of the recombinant proteins.
The general opinion is that IL-6R is present in a few cell types, such as immune cells and hepatocytes, which are directly activated by IL-6 classical signaling [19,48]. In this study, we provide evidence of the existence of membrane-bound IL-6R in L8824 cells but not in CIK cells. IL-6R is important for ligand binding, but it has only a short cytoplasmic domain, and its signal transduction is dependent on the recruitment of gp130 [10,49]. IL-6 is generally believed to activate the JAK/STAT3 pathway, through either soluble or membrane-bound IL-6R. Consistent with this, we found that both rmaIL-6 classical signaling and trans-signaling could trigger STAT3 phosphorylation in a time-dependent manner. However, trans-signaling led to more intense STAT3 phosphorylation than classical signaling. This is also consistent with relevant research in mammals [50,51]. In addition, studies have shown that IL-6-mediated downstream signaling cascade pathways mainly include the JAK/STAT3, MEK/ERK, and PI3K/AKT pathways [52,53,54]. Here, we reported the difference between two signals mediated by rmaIL-6 in different cells. In L8824 cells, classical signaling involves both JAK/STAT3 and MEK/ERK pathways, whereas trans-signaling involves only the JAK/STAT3 pathway. In contrast, in CIK cells, IL-6 trans-signaling could activate both JAK/STAT3 and MEK/ERK pathways. In mammals, several pieces of evidence indicated reciprocal crosstalk between the MEK/ERK pathway and the JAK/STAT3 pathway [55,56]. In addition, IL-6-type cytokines did not activate ERK1/2, but activated STAT3 in some human cells [57,58]. In primary hepatocytes, both IL-6 classical signaling and trans-signaling could activate the JAK/STAT3 pathway but not the MEK/ERK pathway. These results suggest that IL-6 is critical to the activation of the JAK/STAT3 pathway and may not be key to the activation of the MEK/ERK pathway in grass carp cells. Meanwhile, a strong activation of STAT3 may affect ERK phosphorylation to prevent over-immunity in teleost, which is beneficial to maintain the normal operation of the immune system.
It is well known that activation of the JAK/STAT3 pathway leads to STAT3 dimerization and translocation into the nucleus, where it initiates gene transcription [59]. It was shown that socs3 transcription induced by IL-6 lasted at least 48 h in HUVECs cells [60]. In L8824 cells, STAT3 was found to be essential for trans-signaling-mediated expression of socs3a and socs3b. Besides, in L8824 cells and CIK cells, blockade of JAK2 also resulted in complete inhibition of STAT3 phosphorylation as well as of socs3a and socs3b expression induced by trans-signaling. These findings indicate that JAK2 is located upstream of STAT3 in the signaling pathway mediated by IL-6 trans-signaling and that JAK2 is crucial for the induction of socs3a and socs3b. The JAK2/STAT3 inhibitor AG490 reduced hamp mRNA expression even when the cells were exposed to IL-6 [61]. In our study, the expression of hamp was not affected by the rmaIL-6 trans-signaling pathway in the short time, but TG101348 could significantly change its expression in L8824 cells and CIK cells. In previous studies, the JAK inhibitor also acted on other signaling pathways such as MEK/ERK and PI3K/AKT [62,63]. However, whether TG101348 affects hamp expression by inhibiting other signal pathways needs further study.

4. Materials and Methods

4.1. Cell Lines and Fish

Because IL-6 and sIL-6R proteins are conserved between grass carp and blunt snout bream, and blunt snout bream has no stable cell line, grass carp hepatic (L8824) cells and grass carp kidney (CIK) cells (Cell Collection Centre for Freshwater Organisms of Huazhong Agricultural University, Wuhan, China) were selected as model cells in this study. L8824 cells and CIK cells were cultured in M199 medium containing 10% fetal bovine serum with 100 U/mL penicillin and streptomycin (Gibco, NY, USA) and were kept at 28 °C in a 5% CO2 environment.
To amplify the blunt snout bream il-6 (mail-6), il-6r (mail-6r) and grass carp il-6 (ciil-6) cDNA sequences, liver samples from grass carp and blunt snout bream were obtained. Healthy blunt snout bream (0.5–0.7 kg) and grass carp (1.0–1.5 kg) used in the study were obtained from Fisheries College Aquaculture Base, Huazhong Agricultural University, China.

4.2. Isolation and Culture of Hepatocytes

In this study, primary hepatocytes of grass carp were isolated and cultured according to a previous study [64]. Briefly, prior to the isolation of hepatocytes, the blood of the fish was drawn with a syringe. Then, the liver was rapidly isolated and washed several times in ice-cold phosphate-buffered saline (PBS) (Servicebio, Wuhan, China) containing 500 U/mL penicillin and streptomycin. After removal of PBS using sterile pipettes, the samples were cut into small pieces (about 1 mm3). The small pieces of liver were digested with trypsin at 28 °C for 10 min, then the cells were collected, and the process was repeated 3 times. Thereafter, the cell suspension was centrifuged at 400 g for 10 min and washed twice. The harvested cell pellets were resuspended in M199 medium (Gibco, NY, USA) with 10% fetal bovine serum (Gibco, NY, USA) and 100 U/mL penicillin and streptomycin (Gibco, NY, USA) at a density of 1 × 106 cells/mL. Finally, primary hepatocytes were kept at 28 °C in a 5% CO2 environment.

4.3. Sequence Obtainment and Analysis

The mRNA sequences of il-6 (GenBank: KC535507.1), hamp (GenBank: JQ246442.1), socs3a (GenBank: MT925699.1), socs3b (GenBank: MH463452.1) of grass carp and il-6 (GenBank: KJ755058.1), il-6r (GenBank: RXFO00000000.1) of blunt snout bream were derived from NCBI (https://www.ncbi.nlm.nih.gov/nuccore/, accessed on 15 March 2021). The amino acid sequences of IL-6 (GenBank: AHB51767.1), IL-6R (GenBank: AVI05199.1) of grass carp and IL-6 (GenBank: AIG51270.1) of blunt snout bream were derived from NCBI. The blunt snout bream IL-6R CDS and amino acid sequence were deduced using the Open Reading Frame Finder on the NCBI website (http://www.ncbi.nlm.nih.gov/gorf/orfig.cgi, accessed on 10 August 2020). The protein domain features were predicted by using the Simple Modular Architecture Research Tool (SMART) (http://smart.embl-heidelberg.de/, accessed on 9 August 2021). BLASTN and BLASTP (http://www.ncbi.nlm.Nih.gov/BLAST/) (25 June 2021) were used to identify homologous sequences. Multiple amino acid sequences were aligned using the DNAMAN V6 software (LynnonBiosoft, San Ramon, CA, USA). The protein secondary structure and solvent accessibility were predicted by predictprotein (https://predictprotein.org/home, accessed on 9 August 2021).

4.4. RNA Extraction and cDNA Synthesis

Total RNA was extracted with RNAiso Plus (Takara, Shiga, Japan) according to the manufacturer’s instructions. The concentration and quality of total RNA were estimated by means of spectrophotometry with NanoDrop 2000 (Thermo Scientific, Delaware, Waltham, MA, USA) and agarose gel electrophoresis. For quantitative PCR (qPCR) analysis, 1 μg of total RNA was reverse-transcribed using the PrimeScript® RT reagent Kit (Takara, Shiga, Japan) and then stored at −20 °C for further use.

4.5. Expression and Purification of the Recombinant Proteins ciIL-6, maIL-6, and masIL-6R

The mature peptide-coding sequences of ciil-6, mail-6, and masil-6r were amplified by reverse-transcriptase polymerase chain reaction (RT-PCR) using the liver cDNA of grass carp or blunt snout bream as a template. The specific gene primers are listed in Supplementary Table S1. The amplified products were digested by EcoR I/Xho I, BamH I/Hind III, and EcoR I/Hind III, respectively, then ligated into pET-28a/pET-32a, and transfected into BL21 cells (DE3; Tsingke, Jiangsu, China).
The colonies were inoculated into 500 mL of Luria–Bertani (LB) medium containing ampicillin (Amp) or kanamycin (Kan) (50 μg/mL), and the culture solution was incubated at 200 r/min and 37 °C until the OD600 value was 0.5–0.6. Then, the recombinant proteins were induced with isopropyl-β-D-thiogalactoside (IPTG) for 10–12 h prior to harvest. After ultrasonication, the recombinant proteins were affinity-purified using the His-Tagged Inclusion Body Protein Purification Kit (CoWin Biosciences, China) according to the manufacturer’s instructions. The proteins were analyzed by SDS-PAGE and visualized after staining with Coomassie brilliant blue R-250. Then, the purified recombinant proteins were dialyzed and refolded. The concentrations of the recombinant proteins were determined using NanoDrop 2000 (Thermo Scientific, Delaware, Waltham, MA, USA). The recombinant proteins were aliquoted and stored at −80 °C for further use.

4.6. Treatment of Cells

L8824 cells (2.5 × 105 cells/well for 6-well plates) or CIK cells (4.0 × 105 cells/well for 6-well plates) were cultured overnight before treatment. To evaluate the biological activity of recombinant IL-6, L8824 cells were treated with different concentrations of rciIL-6 (0.0, 0.1, 0.5, and 1.0 μg/mL) for 4 h or with rmaIL-6 (0.0, 0.5, 1.0, and 1.5 μg/mL) for 2 h. Then, 1.0 μg/mL rciIL-6 and 1.5 μg/mL rmaIL-6 were selected for the follow-up experiments. Whereafter, L8824 cells and primary hepatocytes were incubated with 1.0 μg/mL rciIL-6 and 1.5 μg/mL rmaIL-6 for different times (2, 4, 8, 12, 24, and 36 h), respectively.
To evaluate the biological activity of rmasIL-6R, CIK cells were treated with rmaIL-6+rmasIL-6R (0.0, 0.5, 1.0, 1.5, 2.0, and 3.0 μg/mL) for 30 min. Then, 1.0 μg/mL rmasIL-6R was selected for the follow-up experiments. After that, L8824 cells, CIK cells, and primary hepatocytes were incubated with rmaIL-6+rmasIL-6R for different times (0, 10, 30, 60, and 120 min). Moreover, L8824 cells and CIK cells were treated with rmaIL-6, rmasIL-6R, and rmaIL-6+rmasIL-6R for 30 min.
The STAT3 inhibitor c188-9 and the JAK2 inhibitor TG101348 were purchased from Selleck (China). Different concentrations of c188-9 (0, 30, 35, 40, 45, 50, and 55 μM) or TG101348 (0.0, 0.2, 0.5, 1.0, 1.5, 2.0, and 2.5 μM) were added into the culture medium, and the cultures were incubated for 10 h to select the proper concentrations for the subsequent treatments. Besides, the cells were retreated with or without rmaIL-6+rmasIL-6R after pretreatment with 35 μM c188-9 and 0.5 μM (L8824 cells) or 2.0 μM (CIK cells) TG101348 for 10 h.
All the above experiments were set up with a blank control and three repetitions. After treatments, the cells were collected to extract total RNA or protein.

4.7. qPCR Analysis

qPCR was performed in a Bio-Rad CFX Connect™ real-time PCR system (Bio-Rad, US). The qPCR mixture consisted of 1.0 μL cDNA template, 7.4 μL nuclease-free water, 10.0 μL LightCycler® 480 SYBR Green I Master (Roche, Switzerland), and 0.8 μL of each forward and reverse primers (10 μM). qPCR was conducted using the following program: 95 °C for 5 min, 40 cycles of 95 °C for 5 s, 60 °C for 20 s, and 72 °C for 20 s, followed by melting curve determination from 65 °C to 95 °C to verify the amplification of a single product. The relative expression levels of the target genes were measured by the 2−ΔΔCt method [65], and 18S rRNA was used as the internal control [66,67,68,69]. The relative expression levels were indicated as fold change. Plasmid construction (ciIL-6, maIL-6, and masIL-6R) and qPCR primers (il-1β, il-6, hamp, socs3a, socs3b, and 18S rRNA) are shown in Supplementary Table S1.

4.8. Protein Extraction and Quantification

The cells were rinsed with PBS and lysed using RIPA lysis buffer (Beyotime, Shanghai, China). To quantify the proteins, the BCA Protein Assay kit was used (Beyotime, Shanghai, China) according to the manufacturer’s instructions, and absorbance at 540 nm was measured using Multiskan-Ascent (Tecan NanoQuant 200, Tecan, Switzerland).

4.9. Western Blot

Cell lysates were mixed with 5 × SDS sample buffer and denatured for 10 min at 95 °C. Next, the protein mixture was loaded into an 8% SDS–PAGE gel, then transferred to the NC membranes (Pall, St. Show Low, AZ, USA) at 200 mA for 1 h. Subsequently, the membranes were blocked with TBST buffer containing 5% BSA or skimmed milk powder for 1.5 h at room temperature, then incubated with anti-STAT3, anti-ERK1/2 (Proteintech, Rosemont, IL, USA), anti-pSTAT3 (Huabio, Hangzhou, China), anti-pERK1/2, anti-β-actin (ABclonal, Wuhan, China) antibodies overnight at 4 °C. On the second day, the membranes were washed with TBST, incubated with goat anti-rabbit secondary antibodies (Yeasen, Shanghai, China) for 1 h at room temperature, and photographed using the Odyssey CLx image system (Li-cor, Lincoln, NE, USA). Finally, the gray value intensities of western blot results were measured by ImageJ software.

4.10. Statistical Analysis

Data are presented as mean ± standard error of the mean (SEM) of three repeated experiments. Statistical significance was analyzed using Student’s t-test or one-way analysis of variance (ANOVA); p < 0.05 indicated significant difference, and p < 0.01 was considered as indicating extremely significant difference.

5. Conclusions

To sum up, rmaIL-6 and rmasIL-6R have biological activity and activate the JAK/STAT3 pathway and the expression of downstream genes. In L8824 cells, IL-6 classical signaling activated both JAK/STAT3 and MEK/ERK pathways, whereas trans-signaling activated only the JAK/STAT3 pathway. In CIK cells, IL-6 trans-signaling activated both JAK/STAT3 and MEK/ERK pathways. In primary hepatocytes, IL-6 classical signaling and trans-signaling only activated the JAK/STAT3 pathway. Therefore, IL-6 mainly acts by activating the JAK/STAT3 pathway. In addition, we demonstrated that activation of the JAK2/STAT3 pathways is essential for IL-6 trans-signaling-induced socs3a and socs3b production in L8824 cells and CIK cells. This study adds to the understanding of the regulation mechanisms of IL-6 classical and trans-signaling in fish, enriches our knowledge of fish immunology, and provides a theoretical basis for the prevention and treatment of fish diseases in the future.

Supplementary Materials

The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/ijms23042019/s1.

Author Contributions

All authors contributed to the article and approved the submitted version. J.W. designed the experiments, analyzed the experiments data, and wrote the manuscript. Q.S. and J.Z. helped to perform the analysis with constructive discussions. H.W. helped to conduct the experiment. H.L. conceived and designed the research plan, guided the experiment, and carried on the manuscript revision. All authors have read and agreed to the published version of the manuscript.

Funding

This project was supported by grants from the National Natural Science Foundation of China (31972781) and the Earmarked Fund for China Agriculture Research System titled as-Staple Freshwater Fishes Industry Technology System (Grant No. CARS-45-08).

Institutional Review Board Statement

We have adhered to all local, national and international regulations and conventions, and we respected normal scientific ethical practices. The specimen used in this study comes from a population that was part of commercially fished individuals intended for human consumption. The animal protocol was approved by the Institutional Animal Care and Use Ethics Committee of Huazhong Agricultural University (Wuhan, China) (HZAUFI-2020-0015).

Informed Consent Statement

Informed consent was obtained from all subjects involved in the study.

Data Availability Statement

All datasets generated for this study are included in the article/Supplementary Materials.

Conflicts of Interest

The authors declare no conflict of interest.

Abbreviations

IL-6Interleukin-6
IL-6RInterleukin-6 receptor
IL-1βInterleukin-1β
HampHepcidin
SOCS3Suppressor of cytokine signaling 3
ciIL-6Grass carp IL-6
ciIL-6RGrass carp IL-6R
maIL-6Blunt snout bream IL-6
maIL-6RBlunt snout bream IL-6R
masIL-6RBlunt snout bream soluble IL-6R receptor
rciIL-6Recombinant grass carp IL-6 protein
rmaIL-6Recombinant blunt snout bream IL-6 protein
rmasIL-6RRecombinant blunt snout bream soluble IL-6 receptor protein
JAKJanus kinases
STATSignal transducer and activator of transcription
gp130Glycoprotein 130
ERKExtracellular-signal-regulated kinase
MEKMitogen-activated protein kinase
PI3KPhosphatidylinositol 3 kinase
AKTProtein kinase b
mIL-6RMembrane-bound IL-6R

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Figure 1. Sequence analysis of IL-6 and sIL-6R in grass carp (C. idellus) and blunt snout bream (M. amblycephala). Sequence alignment of ciIL-6 and maIL-6 (A), cisIL-6R and masIL-6R (B), and secondary structure and solvent accessibility prediction for ciIL-6 and maIL-6 (C). The consensus sequence amino acids are shown in solid black. The differences in solvent accessibility are boxed, and the difference in secondary structure is indicated by arrows.
Figure 1. Sequence analysis of IL-6 and sIL-6R in grass carp (C. idellus) and blunt snout bream (M. amblycephala). Sequence alignment of ciIL-6 and maIL-6 (A), cisIL-6R and masIL-6R (B), and secondary structure and solvent accessibility prediction for ciIL-6 and maIL-6 (C). The consensus sequence amino acids are shown in solid black. The differences in solvent accessibility are boxed, and the difference in secondary structure is indicated by arrows.
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Figure 2. Production and biological activity of recombinant IL-6 of grass carp (rciIL-6) and blunt snout bream (rmaIL-6). SDS-PAGE of rciIL-6 (A) and rmaIL-6 (B) proteins. Lane 1: molecular mass marker; Lane 2: whole-cell lysate of non-induced E. coli; Lane 3: whole-cell lysate of induced E. coli containing the recombinant proteins; Lane 4: purified and refolded recombinant proteins. L8824 cells were treated with different concentrations of rciIL-6 (C) or rmaIL-6 (D). L8824 cells were treated with different concentrations of rciIL-6 or mail-6 for different times (E). The hamp, il-6, il-1β, socs3a, and socs3b mRNA were quantified by qPCR. Gene expression was normalized relative to the reference gene 18S rRNA. Fold changes were calculated by comparing the average gene expression of the treatment groups with that of the corresponding control groups (HI, heat-inactivated protein). (C,D) Student’s t-test was used to determine the significance of differences between the experimental and the control groups. (E), One-way analysis of variance (ANOVA) was used to analyze the differences among different time points. Data are presented as mean ± SEM of at least three replicates for each experiment. * p < 0.05, ** p < 0.01.
Figure 2. Production and biological activity of recombinant IL-6 of grass carp (rciIL-6) and blunt snout bream (rmaIL-6). SDS-PAGE of rciIL-6 (A) and rmaIL-6 (B) proteins. Lane 1: molecular mass marker; Lane 2: whole-cell lysate of non-induced E. coli; Lane 3: whole-cell lysate of induced E. coli containing the recombinant proteins; Lane 4: purified and refolded recombinant proteins. L8824 cells were treated with different concentrations of rciIL-6 (C) or rmaIL-6 (D). L8824 cells were treated with different concentrations of rciIL-6 or mail-6 for different times (E). The hamp, il-6, il-1β, socs3a, and socs3b mRNA were quantified by qPCR. Gene expression was normalized relative to the reference gene 18S rRNA. Fold changes were calculated by comparing the average gene expression of the treatment groups with that of the corresponding control groups (HI, heat-inactivated protein). (C,D) Student’s t-test was used to determine the significance of differences between the experimental and the control groups. (E), One-way analysis of variance (ANOVA) was used to analyze the differences among different time points. Data are presented as mean ± SEM of at least three replicates for each experiment. * p < 0.05, ** p < 0.01.
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Figure 3. (A), SDS-PAGE of the rmasIL-6R produced from E. coli. Lane 1: molecular mass marker; Lane 2: whole-cell lysate of non-induced E. coli; Lane 3: whole-cell lysate of induced E. coli containing the recombinant protein; Lane 4: purified and refolded recombinant protein. (B), STAT3 phosphorylation in CIK cells treated with rmaIL-6+rmasIL-6R for 30 min. (C), Phosphorylation of STAT3 and ERK1/2 in L8824 cells treated with rmaIL-6 alone or in combination with rmasIL-6R. (E), Phosphorylation of STAT3 and ERK1/2 in CIK cells treated with rmaIL-6+rmasIL-6R. The signals of phosphorylated proteins and total proteins were first normalized to β-actin, and the ratios between phosphorylated protein and total protein were calculated. Data are presented as mean ± SEM of at least three replicates for each experiment (D,F). * p < 0.05, ** p < 0.01.
Figure 3. (A), SDS-PAGE of the rmasIL-6R produced from E. coli. Lane 1: molecular mass marker; Lane 2: whole-cell lysate of non-induced E. coli; Lane 3: whole-cell lysate of induced E. coli containing the recombinant protein; Lane 4: purified and refolded recombinant protein. (B), STAT3 phosphorylation in CIK cells treated with rmaIL-6+rmasIL-6R for 30 min. (C), Phosphorylation of STAT3 and ERK1/2 in L8824 cells treated with rmaIL-6 alone or in combination with rmasIL-6R. (E), Phosphorylation of STAT3 and ERK1/2 in CIK cells treated with rmaIL-6+rmasIL-6R. The signals of phosphorylated proteins and total proteins were first normalized to β-actin, and the ratios between phosphorylated protein and total protein were calculated. Data are presented as mean ± SEM of at least three replicates for each experiment (D,F). * p < 0.05, ** p < 0.01.
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Figure 4. Phosphorylation of STAT3 and ERK1/2 in primary hepatocytes treated with rmaIL-6 alone (A) or in combination with rmasIL-6R (B). The signals of phosphorylated proteins and total proteins were first normalized to β-actin, and the ratios between phosphorylated protein and total protein were calculated (C,D). Data are presented as mean ± SEM of at least three replicates for each experiment. * p < 0.05.
Figure 4. Phosphorylation of STAT3 and ERK1/2 in primary hepatocytes treated with rmaIL-6 alone (A) or in combination with rmasIL-6R (B). The signals of phosphorylated proteins and total proteins were first normalized to β-actin, and the ratios between phosphorylated protein and total protein were calculated (C,D). Data are presented as mean ± SEM of at least three replicates for each experiment. * p < 0.05.
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Figure 5. STAT3 phosphorylation in response to rmaIL-6 stimulation in CIK cells (A), L8824 cells (B), and primary hepatocytes (C). A representative blot containing phosphorylated proteins, total proteins, and β-actin is shown for each pathway (left column). Ratios of phosphorylated proteins to total proteins were calculated. Data are presented as mean ± SEM of at least three replicates for each experiment (right column). * p < 0.05, ** p < 0.01.
Figure 5. STAT3 phosphorylation in response to rmaIL-6 stimulation in CIK cells (A), L8824 cells (B), and primary hepatocytes (C). A representative blot containing phosphorylated proteins, total proteins, and β-actin is shown for each pathway (left column). Ratios of phosphorylated proteins to total proteins were calculated. Data are presented as mean ± SEM of at least three replicates for each experiment (right column). * p < 0.05, ** p < 0.01.
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Figure 6. Inhibitory effects of c188-9 or TG101348 on the phosphorylation of STAT3 and ERK1/2. STAT3 phosphorylation after treatment with different concentrations of c188-9 in L8824 (A) and CIK cells (C). Phosphorylation of STAT3 and ERK1/2 after treatment with different concentrations of TG101348 in L8824 (B) and CIK (D) cells.
Figure 6. Inhibitory effects of c188-9 or TG101348 on the phosphorylation of STAT3 and ERK1/2. STAT3 phosphorylation after treatment with different concentrations of c188-9 in L8824 (A) and CIK cells (C). Phosphorylation of STAT3 and ERK1/2 after treatment with different concentrations of TG101348 in L8824 (B) and CIK (D) cells.
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Figure 7. Effects of different inhibitors on IL-6 trans-signaling. (A), Effect of c188-9 on STAT3 phosphorylation (left) and gene expression (right) regulated by rmaIL-6+rmasIL-6R in L8824 cells. (B), Effect of TG101348 on STAT3 phosphorylation (left) and gene expression (right) in the downstream pathway activated by rmaIL-6+rmasIL-6R in L8824 cells. (C), Effect of TG101348 on STAT3 phosphorylation (left) and gene expression (right) regulated by rmaIL-6+rmasIL-6R in CIK cells. Data are presented as mean ± SEM of at least three replicates for each experiment. * p < 0.05, ** p < 0.01, ns no significance.
Figure 7. Effects of different inhibitors on IL-6 trans-signaling. (A), Effect of c188-9 on STAT3 phosphorylation (left) and gene expression (right) regulated by rmaIL-6+rmasIL-6R in L8824 cells. (B), Effect of TG101348 on STAT3 phosphorylation (left) and gene expression (right) in the downstream pathway activated by rmaIL-6+rmasIL-6R in L8824 cells. (C), Effect of TG101348 on STAT3 phosphorylation (left) and gene expression (right) regulated by rmaIL-6+rmasIL-6R in CIK cells. Data are presented as mean ± SEM of at least three replicates for each experiment. * p < 0.05, ** p < 0.01, ns no significance.
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Wang, J.; Sun, Q.; Zhang, J.; Wang, H.; Liu, H. Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R. Int. J. Mol. Sci. 2022, 23, 2019. https://doi.org/10.3390/ijms23042019

AMA Style

Wang J, Sun Q, Zhang J, Wang H, Liu H. Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R. International Journal of Molecular Sciences. 2022; 23(4):2019. https://doi.org/10.3390/ijms23042019

Chicago/Turabian Style

Wang, Jixiu, Qianhui Sun, Jian Zhang, Huanling Wang, and Hong Liu. 2022. "Classical Signaling and Trans-Signaling Pathways Stimulated by Megalobrama amblycephala IL-6 and IL-6R" International Journal of Molecular Sciences 23, no. 4: 2019. https://doi.org/10.3390/ijms23042019

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