Low Light Increases the Abundance of Light Reaction Proteins: Proteomics Analysis of Maize (Zea mays L.) Grown at High Planting Density

Zheng, Bin; Zhao, Wei; Ren, Tinghu; Zhang, Xinghui; Ning, Tangyuan; Liu, Peng; Li, Geng

doi:10.3390/ijms23063015

Open AccessArticle

Low Light Increases the Abundance of Light Reaction Proteins: Proteomics Analysis of Maize (Zea mays L.) Grown at High Planting Density

by

Bin Zheng

^†,

Wei Zhao

^†,

Tinghu Ren

,

Xinghui Zhang

,

Tangyuan Ning

,

Peng Liu

and

Geng Li

^*

College of Agronomy, Shandong Agricultural University, Tai’an 271018, China

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Int. J. Mol. Sci. 2022, 23(6), 3015; https://doi.org/10.3390/ijms23063015

Submission received: 20 December 2021 / Revised: 27 February 2022 / Accepted: 8 March 2022 / Published: 10 March 2022

(This article belongs to the Special Issue Response of Photosynthesis to Abiotic Stresses)

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Abstract

Maize (Zea mays L.) is usually planted at high density, so most of its leaves grow in low light. Certain morphological and physiological traits improve leaf photosynthetic capacity under low light, but how light absorption, transmission, and transport respond at the proteomic level remains unclear. Here, we used tandem mass tag (TMT) quantitative proteomics to investigate maize photosynthesis-related proteins under low light due to dense planting, finding increased levels of proteins related to photosystem II (PSII), PSI, and cytochrome b₆f. These increases likely promote intersystem electron transport and increased PSI end electron acceptor abundance. OJIP transient curves revealed increases in some fluorescence parameters under low light: quantum yield for electron transport (φE_o), probability that an electron moves beyond the primary acceptor Q_A⁻ (ψ_o), efficiency/probability of electron transfer from intersystem electron carriers to reduction end electron acceptors at the PSI acceptor side (δR_o), quantum yield for reduction of end electron acceptors at the PSI acceptor side (φR_o), and overall performance up to the PSI end electron acceptors (PI_total). Thus, densely planted maize shows elevated light utilization through increased electron transport efficiency, which promotes coordination between PSII and PSI, as reflected by higher apparent quantum efficiency (AQE), lower light compensation point (LCP), and lower dark respiration rate (R_d).

Keywords: photosynthesis; low light; chlorophyll fluorescence; proteomics; maize; dense planting

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MDPI and ACS Style

Zheng, B.; Zhao, W.; Ren, T.; Zhang, X.; Ning, T.; Liu, P.; Li, G. Low Light Increases the Abundance of Light Reaction Proteins: Proteomics Analysis of Maize (Zea mays L.) Grown at High Planting Density. Int. J. Mol. Sci. 2022, 23, 3015. https://doi.org/10.3390/ijms23063015

AMA Style

Zheng B, Zhao W, Ren T, Zhang X, Ning T, Liu P, Li G. Low Light Increases the Abundance of Light Reaction Proteins: Proteomics Analysis of Maize (Zea mays L.) Grown at High Planting Density. International Journal of Molecular Sciences. 2022; 23(6):3015. https://doi.org/10.3390/ijms23063015

Chicago/Turabian Style

Zheng, Bin, Wei Zhao, Tinghu Ren, Xinghui Zhang, Tangyuan Ning, Peng Liu, and Geng Li. 2022. "Low Light Increases the Abundance of Light Reaction Proteins: Proteomics Analysis of Maize (Zea mays L.) Grown at High Planting Density" International Journal of Molecular Sciences 23, no. 6: 3015. https://doi.org/10.3390/ijms23063015

APA Style

Zheng, B., Zhao, W., Ren, T., Zhang, X., Ning, T., Liu, P., & Li, G. (2022). Low Light Increases the Abundance of Light Reaction Proteins: Proteomics Analysis of Maize (Zea mays L.) Grown at High Planting Density. International Journal of Molecular Sciences, 23(6), 3015. https://doi.org/10.3390/ijms23063015

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Low Light Increases the Abundance of Light Reaction Proteins: Proteomics Analysis of Maize (Zea mays L.) Grown at High Planting Density

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