HDAC Inhibitor Sodium Butyrate Attenuates the DNA Repair in Transformed but Not in Normal Fibroblasts
Abstract
:1. Introduction
2. Results
2.1. Histone Deacetylase Inhibitor Sodium Butyrate Prolongs Genotoxic-Induced DNA Double-Strand Breaks in E1A+Ras-Transformed Cells but Not in Normal Cells
2.2. Sodium Butyrate Prolongs a Doxorubicin-Induced Persistence of the Phosphorylated Histone H2AX in Transformed Cells
2.3. The Histone Deacetylase Inhibitor Sodium Butyrate Inhibits the Repair of Exogenous DNA in Transformed Cells but Not in Normal Cells
2.4. NaBut Interferes with DNA Repair Machinery in Transformed Cells
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. Comet Assay
4.3. Host-Cell Reactivation Assay (HCR)
4.4. Immunoblotting
4.5. Immunoprecipitation
4.6. Immunofluorescence
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Gnedina, O.O.; Morshneva, A.V.; Skvortsova, E.V.; Igotti, M.V. HDAC Inhibitor Sodium Butyrate Attenuates the DNA Repair in Transformed but Not in Normal Fibroblasts. Int. J. Mol. Sci. 2022, 23, 3517. https://doi.org/10.3390/ijms23073517
Gnedina OO, Morshneva AV, Skvortsova EV, Igotti MV. HDAC Inhibitor Sodium Butyrate Attenuates the DNA Repair in Transformed but Not in Normal Fibroblasts. International Journal of Molecular Sciences. 2022; 23(7):3517. https://doi.org/10.3390/ijms23073517
Chicago/Turabian StyleGnedina, Olga O., Alisa V. Morshneva, Elena V. Skvortsova, and Maria V. Igotti. 2022. "HDAC Inhibitor Sodium Butyrate Attenuates the DNA Repair in Transformed but Not in Normal Fibroblasts" International Journal of Molecular Sciences 23, no. 7: 3517. https://doi.org/10.3390/ijms23073517