Sturgeon Chondroitin Sulfate Restores the Balance of Gut Microbiota in Colorectal Cancer Bearing Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Anti-Tumor Effect of SCS in HT-29 Transplanted Tumor Mice
2.2. Effect of SCS on Colon Tissue in Colorectal Cancer Graft Mice
2.3. SCS Treatment Alters the Structure of Gut Microbiota
2.4. Effect of SCS on Gut Metabolites in Tumor-Bearing Mice
2.4.1. Fecal Metabolic Profiling by UHPLC-Q-TOF/MS
2.4.2. Detection and Identification of Differentially Produced Metabolites after SCS Treatment
2.4.3. SCS Inhibits the Production of Amino Acid Metabolites
3. Materials and Methods
3.1. Reagents and Materials
3.2. Animal Experiment Design
3.3. Gut Microbiota Analysis
3.4. LC-MS/MS Analysis (HLIC/MS)
3.5. Differential Metabolites Identification and Pathway Analysis
3.6. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wu, R.; Shen, Q.; Li, P.; Shang, N. Sturgeon Chondroitin Sulfate Restores the Balance of Gut Microbiota in Colorectal Cancer Bearing Mice. Int. J. Mol. Sci. 2022, 23, 3723. https://doi.org/10.3390/ijms23073723
Wu R, Shen Q, Li P, Shang N. Sturgeon Chondroitin Sulfate Restores the Balance of Gut Microbiota in Colorectal Cancer Bearing Mice. International Journal of Molecular Sciences. 2022; 23(7):3723. https://doi.org/10.3390/ijms23073723
Chicago/Turabian StyleWu, Ruiyun, Qian Shen, Pinglan Li, and Nan Shang. 2022. "Sturgeon Chondroitin Sulfate Restores the Balance of Gut Microbiota in Colorectal Cancer Bearing Mice" International Journal of Molecular Sciences 23, no. 7: 3723. https://doi.org/10.3390/ijms23073723