Genome-Wide Characterization and Evolutionary Expansion of Poplar NAC Transcription Factors and Their Tissue-Specific Expression Profiles under Drought
Abstract
:1. Introduction
2. Results
2.1. Identification of PtrNAC Family Genes in Poplar
2.2. Chromosomal Distributions and Collinearity Analysis of PtrNACs
2.3. Phylogenetic Tree and Gene Structures of PtrNACs
2.4. Cis-Element Analysis of PtrNAC Promoters
2.5. Cloning of Poplar NAC Genes Cloning and Analysis of PtrNAC Expression Patterns in Response to Drought Stress
2.6. Subcellular Localization Prediction and Verification
3. Discussion
4. Materials and Methods
4.1. Genomic Data Retrieval and Poplar NAC Gene Identification
4.2. Multiple Alignment and Phylogenetic Analysis
4.3. PtNACs Gene Structures and Conserved Motifs
4.4. Subcellular Localization Prediction and Verification
4.5. Chromosomal Distribution and Gene Duplication Analysis
4.6. Cis-Element Analysis
4.7. RNA-Seq Acquisition and Expression Analysis
4.8. Plant Materials and Treatments with Drought Stress
4.9. RNA Isolation and qRT-PCR
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Meng, L.; Chen, S.; Li, D.; Huang, M.; Zhu, S. Genome-Wide Characterization and Evolutionary Expansion of Poplar NAC Transcription Factors and Their Tissue-Specific Expression Profiles under Drought. Int. J. Mol. Sci. 2023, 24, 253. https://doi.org/10.3390/ijms24010253
Meng L, Chen S, Li D, Huang M, Zhu S. Genome-Wide Characterization and Evolutionary Expansion of Poplar NAC Transcription Factors and Their Tissue-Specific Expression Profiles under Drought. International Journal of Molecular Sciences. 2023; 24(1):253. https://doi.org/10.3390/ijms24010253
Chicago/Turabian StyleMeng, Lu, Siyuan Chen, Dawei Li, Minren Huang, and Sheng Zhu. 2023. "Genome-Wide Characterization and Evolutionary Expansion of Poplar NAC Transcription Factors and Their Tissue-Specific Expression Profiles under Drought" International Journal of Molecular Sciences 24, no. 1: 253. https://doi.org/10.3390/ijms24010253