Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation
Abstract
:1. Introduction
2. Results
2.1. The Physicochemical Properties of Sericin
2.2. Protein Composition Analysis of Sericin Samples
2.3. Sericin Is a Multifunctional Biomacromolecule
2.4. Sericin Suppressed LPS-Induced Immune Responses through the Pathogen Recognition Receptors (PRRs) Signaling Pathways
2.5. Molecular Mechanism of Sericin Inhibiting LPS-Induced Inflammation
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Sericin Extract
4.3. Cell Counting Kit-8 (CCK- 8) Assay
4.4. Ocular Irritant and Corrosive HET-CAM Test
4.5. Luciferase Assay
4.6. ELISA Assay
4.7. Quantitative Real-Time PCR
4.8. Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)
4.9. RNA-Seq
4.10. Protein Identification
4.11. SDS-PAGE
4.12. Western Blot
4.13. Aerobic Bacterial Count Assay
4.14. Solid Content/Protein Content Assay
4.15. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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No. | Material State | Color | Smell | pH * | Aerobic Bacterial Count (CFU/mL (g)) |
---|---|---|---|---|---|
S1 | Powder | Pale Yellow | Strong Smell | 5.36 | <10 |
S2 | Liquid | Brown | Moderate Smell | 5.6 | <10 |
S3 | Liquid | Brown | Strong Smell | 5.44 | <10 |
SS1 | Powder | Pale yellow | Light Smell | 4.8 | <10 |
SS2 | Powder | Pale yellow | Light Smell | 4.53 | <10 |
Protein Classification | Protein ID | Data Base | S1 (%) | S2 (%) | S3 (%) | SS1 (%) | SS2 (%) |
---|---|---|---|---|---|---|---|
Sericin 1 | NP_001037506.1 | NCBI | 49.46274901 | 5.335532292 | 8.839730442 | 53.30999038 | 68.01922871 |
XP_012552503.2 | NCBI | 0 | 0 | 0 | 5.771091599 | 3.146896395 | |
XP_004934087.2 | NCBI | 0 | 0 | 0 | 2.572240592 | 1.684852375 | |
BGIBMGA002689 | Silkdb 2.0 | 15.31242542 | 0.017105123 | 0 | 31.98284251 | 21.86263104 | |
Sericin 2 | NP_001166287.1 | NCBI | 1.950921623 | 0.018734328 | 21.94072201 | 0.336459243 | 0.596049957 |
Enzymes | XP_021208623.1 | NCBI | 1.259668974 | 0 | 61.73346493 | 0.096624902 | 0.077723845 |
BGIBMGA014024 | Silkdb 2.0 | 0 | 0.004999576 | 0 | 0 | 0.004847294 | |
Protease inhibitors | BGIBMGA009092 | Silkdb 2.0 | 6.380469726 | 0 | 6.315789474 | 0 | 0 |
NP_001139708.1 | NCBI | 0.764392908 | 0.150414115 | 0.227766897 | 0 | 0 | |
Unannotated Proteins | BGIBMGA000013 | Silkdb 2.0 | 23.46805554 | 93.68313431 | 0.913997563 | 4.734896657 | 4.115853659 |
BGIBMGA005069 | Silkdb 2.0 | 1.401316805 | 0.790080258 | 0.028528691 | 1.195854114 | 0.49191672 | |
Sericin content ratio | 66.72609605 | 5.371371743 | 30.78045245 | 93.97262433 | 94.71360853 |
NO. | Score of 6 Replicates | Total Score | Results | |||||
---|---|---|---|---|---|---|---|---|
5% SS1 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | Non-irritant |
7.5% SS1 | 1 | 1 | 0 | 1 | 0 | 0 | 3 | Non-irritant |
10% SS1 | 1 | 1 | 1 | 2 | 1 | 0 | 6 | Non-irritant |
5% SS2 | 1 | 0 | 1 | 1 | 0 | 1 | 4 | Non-irritant |
7.5% SS2 | 1 | 0 | 1 | 0 | 1 | 1 | 4 | Non-irritant |
10% SS2 | 1 | 1 | 2 | 1 | 1 | 2 | 8 | Non-irritant |
Blank (Saline) | 0 | 0 | 0 | 0 | 0 | 0 | 0 | Non-irritant |
0.1M NaOH | 9 | 12 | 9 | 12 | 6 | 9 | 57 | Severe irritant |
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Sun, Y.; Shi, W.; Zhang, Q.; Guo, H.; Dong, Z.; Zhao, P.; Xia, Q. Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation. Int. J. Mol. Sci. 2023, 24, 259. https://doi.org/10.3390/ijms24010259
Sun Y, Shi W, Zhang Q, Guo H, Dong Z, Zhao P, Xia Q. Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation. International Journal of Molecular Sciences. 2023; 24(1):259. https://doi.org/10.3390/ijms24010259
Chicago/Turabian StyleSun, Yueting, Wenyu Shi, Quan Zhang, Haiqiong Guo, Zhaoming Dong, Ping Zhao, and Qingyou Xia. 2023. "Multi-Omics Integration to Reveal the Mechanism of Sericin Inhibiting LPS-Induced Inflammation" International Journal of Molecular Sciences 24, no. 1: 259. https://doi.org/10.3390/ijms24010259