Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production
Abstract
:1. Introduction
2. Results
2.1. Expression of GORASP2 Was Increased in the Myometrium during Labor
2.2. GORASP2 Was Involved in the Contractile Function of hMSMCs
2.3. GORASP2 on the Biological Functions of hMSMCs
2.4. GORASP2 Maintained the Energy Metabolism Levels of hMSMCs
3. Discussion
4. Materials and Methods
4.1. Antibodies
4.2. Subjects and Tissue Collection
4.3. Western Blot
4.4. Transmission Electron Photography
4.5. Primary Culture and hMSMCs Identification
4.6. Immunofluorescence
4.7. Cell Transfection
4.8. Cell Contraction Assay
4.9. Reactive Oxygen Species and Apoptosis Assay
4.10. Cell Cycle Detection
4.11. RNA Sequencing (RNA-Seq) and Data Analysis
4.12. Reverse Transcription Quantitative Polymerase Chain Reaction (RT-qPCR)
4.13. Mitochondrial Stress Assay
4.14. ATP Detection Assay
4.15. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yang, F.; Chen, L.; Wen, B.; Wang, X.; Wang, L.; Ji, K.; Liu, H. Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production. Int. J. Mol. Sci. 2023, 24, 10116. https://doi.org/10.3390/ijms241210116
Yang F, Chen L, Wen B, Wang X, Wang L, Ji K, Liu H. Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production. International Journal of Molecular Sciences. 2023; 24(12):10116. https://doi.org/10.3390/ijms241210116
Chicago/Turabian StyleYang, Fan, Lina Chen, Bolun Wen, Xiaodi Wang, Lele Wang, Kaiyuan Ji, and Huishu Liu. 2023. "Golgi Reassembly Stacking Protein 2 Modulates Myometrial Contractility during Labor by Affecting ATP Production" International Journal of Molecular Sciences 24, no. 12: 10116. https://doi.org/10.3390/ijms241210116